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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE preprint 2017-09-21
| MitoEAGLE preprint 2017-10-07(07). The protonmotive force and respiratory control: Building blocks of mitochondrial physiology Part 1. - http://www.mitoeagle.org/index.php/MitoEAGLE_preprint_2017-09-21. |
» Last update 2017-10-07: 
- » Versions
Corresponding author:, Gnaiger E, Contributing co-authors:, Ahn B, Alves MG, Amati F, Asander Frostner E, Battino M, Beard DA, Ben-Shachar D, Bishop D, Breton S, Brown GC, Brown RA, Buettner GR, Cervinkova Z, Chicco AJ, Coen PM, Collins JL, Crisostomo L, Davis MS, Dias T, Distefano G, Doerrier C, Ehinger J, Elmer E, Fell DA, Filipovska A, Fisher J, Garcia-Roves PM, Garcia-Souza LF, Genova ML, Gonzalo H, Goodpaster BH, Gorr TA, Han J, Harrison DK, Hellgren KT, Hernansanz P, Holland O, Hoppel CL, Iglesias-Gonzalez J, Irving BA, Iyer S, Jansen-Duerr P, Jespersen NR, Jha RK, Kaeaembre T, Kane DA, Kappler L, Keijer J, Komlodi T, Krako Jakovljevic N, Kuang J, Labieniec-Watala M, Lai N, Laner V, Lee HK, Lemieux H, Lerfall J, Lucchinetti E, MacMillan-Crow LA, Makrecka-Kuka M, Meszaros AT, Moisoi N, Molina AJA, Montaigne D, Moore AL, Murray AJ, Newsom S, Nozickova K, O'Gorman D, Oliveira PF, Oliveira PJ, Orynbayeva Z, Pak YK, Palmeira CM, Patel HH, Pesta D, Petit PX, Pichaud N, Pirkmajer S, Porter RK, Pranger F, Prochownik EV, Radenkovic F, Reboredo P, Renner-Sattler K, Robinson MM, Rohlena J, Røsland GV, Rossiter HB, Salvadego D, Scatena R, Schartner M, Scheibye-Knudsen M, Schilling JM, Schlattner U, Schoenfeld P, Scott GR, Singer D, Sobotka O, Spinazzi M, Stocker R, Sumbalova Z, Suravajhala P, Tanaka M, Tandler B, Tepp K, Tomar D, Towheed A, Trivigno C, Tronstad KJ, Tyrrell DJ, Velika B, Vendelin M, Vercesi AE, Victor VM, Ward ML, Watala C, Wei YH, Wieckowski MR, Wohlwend M, Wolff J, Wuest RCI, Zaugg M, Zorzano A, Supporting co-authors:, Arandarcikaite O, Bailey DM, Bakker BM, Batista Ferreira J, Bernardi P, Boetker HE, Borsheim E, Borutaite V, Bouitbir J, Calabria E, Calbet JA, Carvalho E, Chaurasia B, Clementi E, Collin A, Das AM, De Palma C, Dubouchaud H, Duchen MR, Durham WJ, Dyrstad SE, Engin AB, Fornaro M, Gan Z, Garlid KD, Garten A, Genova ML, Gourlay CW, Granata C, Haas CB, Haavik J, Haendeler J, Hand SC, Hepple RT, Hickey AJ, Hoel F, Kainulainen H, Keppner G, Khamoui AV, Klingenspor M, Koopman WJH, Kowaltowski AJ, Krajcova A, Lenaz G, Malik A, Markova M, Mazat JP, Menze MA, Methner A, Muntane J, Muntean DM, Neuzil J, Oliveira MT, Pallotta ML, Parajuli N, Pettersen IKN, Pulinilkunnil T, Ropelle ER, Salin K, Sandi C, Sazanov LA, Siewiera K, Silber AM, Skolik R, Smenes BT, Soares FAA, Sokolova I, Sonkar VK, Stankova P, Stier A, Swerdlow RH, Szabo I, Trifunovic A, Thyfault JP, Tretter L, Trougakos IP, Vieyra A, Votion DM, Williams C, Zaugg K (2017) MitoEAGLE preprint
Abstract: Clarity of concepts and consistency of nomenclature are trademarks of a research field across its specializations, facilitating transdisciplinary communication and education. As research and knowledge of mitochondrial physiology expand, the necessity for harmonizing nomenclature concerning mitochondrial respiratory states and rates has become apparent. Peter Mitchell’s concept of the protonmotive force establishes the link between the electrical and chemical components of energy transformation and its coupling in oxidative phosphorylation. This unifying concept provides the framework for developing a consistent terminology of mitochondrial physiology and bioenergetics. We follow IUPAC guidelines on general terms of physical chemistry, extended by concepts of open systems and irreversible thermodynamics. We align the nomenclature of classical bioenergetics on respiratory states with a concept-driven constructive terminology to address the meaning of each respiratory state. Standards for evaluation of respiratory coupling states must be followed for the development of databases of mitochondrial respiratory function in species, tissues and cells studied under diverse physiological and experimental conditions. • Keywords: Mitochondrial respiratory control, coupling control, mitochondrial preparations, protonmotive force, chemiosmotic theory, oxidative phosphorylation, OXPHOS, efficiency, electron transfer, ET; proton leak, LEAK, residual oxygen consumption, ROX, State 2, State 3, State 4, normalization, flow, flux • Bioblast editor: Gnaiger E
- Citation:
- MitoEAGLE preprint 2017-09-21(Version #). The protonmotive force and respiratory control: Building blocks of mitochondrial physiology Part 1. - http://www.mitoeagle.org/index.php/MitoEAGLE_preprint_2017-09-21
Authors
- Contributing co-authors: Confirming to have read the final manuscript, possibly to have made additions or suggestions for improvement, and to agree to implement the recommendations into future manuscripts, presentations and teaching materials. (alphabetical, to be extended)
- Supporting co-authors: Confirming to have read the final manuscript, and to agree to implement the recommendations into future manuscripts, presentations and teaching materials. (alphabetical, to be extended)
Discussion
Concept: COST Action MitoEAGLE
- Phase 1: 44 versions until 2017-09-18
- Phase 2 (2017-09-21 ongoing): MitoEAGLE preprint - with updates on route to let the final publication fly. Feedback, suggestions, and confirmation from co-authors.
- Phase 3 (2017-10-24): Manuscript submission to a preprint server, such as BioRxiv. Contact the editor(s) of our finally targeted journal(s), to obtain a first opinion if submission to this journal will be adequate. Cell Metabolism and BBA have been mentioned as possible preferences.
- Phase 4: Manuscript submission for journal publication.
Labels: MiParea: Respiration, mt-Awareness
Preparation: Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria
Enzyme: Marker enzyme
Regulation: Coupling efficiency;uncoupling, Flux control, mt-Membrane potential, Uncoupler
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
Pathway: ROX
