MiPNet17.04 CitrateSynthase: Difference between revisions

Latest revision as of 15:56, 1 February 2021

MiPNet17.04 CitrateSynthase

Laboratory protocol: Citrate synthase a mitochondrial marker enzyme.

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Oroboros (2020-04-18) Mitochondr Physiol Network

Abstract: Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. Mitochondr Physiol Network 17.04(04):1-12.

Version 04: New Section 6. Normalization of respiratory flux for CS activity • Keywords: Normalization of rate

• O2k-Network Lab: AT Innsbruck Oroboros

Open the pdf document above.

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Author contributions

This MiPNet Protocol was originally prepared by Kuznetsov AV, Lassing B, Gnaiger E as MiPNet08.14 (2003) and further edited by Wiethüchter A (2011). MiPNet17.04 (2012 version) was edited by Eigentler A, Draxl A, Gnaiger E; contribution to K-Regio project MitoCom Tyrol, funded by the Tyrolian Government and the European Regional Development Fund (ERDF).

Last updates: contribution to H2020 Project NextGen-O2k.

Bioblast links: Normalization - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Rate

» Normalization of rate

» Flow

» Oxygen flow

» Flux

» Oxygen flux

» Flux control ratio

» Coupling-control ratio

» Pathway control ratio

» Flux control efficiency

Quantities for normalization

» Count in contrast to Number

» Mitochondrial marker

» O2k-Protocols: mitochondrial and marker-enzymes

» Citrate synthase activity

General

» Extensive quantity

» Specific quantity

» Advancement

» Motive unit

» Iconic symbols

Related keyword lists

» Keywords: Concentration and pressure

Labels: MiParea: Respiration, Instruments;methods

Preparation: Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, Enzyme Enzyme: Marker enzyme

HRR: Oxygraph-2k, O2k-Protocol

Mt- and marker-enzymes, Normalization of rate

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+{{OROBOROS header page name}}
 {{Publication
 |title=[[Image:O2k-Protocols.jpg|right|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols]] Laboratory protocol: Citrate synthase a mitochondrial marker enzyme.
@@ Line 11: / Line 12: @@
 |mipnetlab=AT Innsbruck Oroboros
 }}
+{{MiPNet pdf page linking to MitoPedia}}
+== Author contributions ==
+:::: This MiPNet Protocol was originally prepared by Kuznetsov AV, Lassing B, Gnaiger E as MiPNet08.14 (2003) and further edited by Wiethüchter A (2011). MiPNet17.04 (2012 version) was edited by Eigentler A, Draxl A, Gnaiger E; contribution to K-Regio project ''MitoCom Tyrol'', funded by the Tyrolian Government and the European Regional Development Fund (ERDF).
+:::: Last updates: contribution to H2020 Project NextGen-O2k.
+{{Template:Keywords: Normalization}}
 {{Labeling
 |area=Respiration, Instruments;methods
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 |enzymes=Marker enzyme
 |instruments=Oxygraph-2k, O2k-Protocol
-|additional=Mt- and marker-enzymes
+|additional=Mt- and marker-enzymes, Normalization of rate
 }}
-{{OROBOROS header page name}}
-{{MiPNet pdf page linking to MitoPedia}}
-{{Template:Keywords: Normalization}}