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• NADH electron transfer-pathway state
  ...id]]). In the N-pathway control state [[Electron-transfer-pathway state|ET pathway level 4]] is active. |info=[[Electron-transfer-pathway state]], [[Gnaiger 2020 BEC MitoPathways]]
  3 KB (409 words) - 08:06, 25 November 2023
• NADH Electron transfer-pathway state
  #REDIRECT [[NADH electron transfer-pathway state]]
  50 bytes (5 words) - 08:10, 26 August 2020

Page text matches

• N-pathway control state
  #REDIRECT [[NADH electron transfer-pathway state]]
  50 bytes (5 words) - 13:49, 26 August 2020
• N-pathway control
  #REDIRECT [[NADH electron transfer-pathway state]]
  50 bytes (5 words) - 13:49, 26 August 2020
• N-linked substrate state
  #REDIRECT [[NADH electron transfer-pathway state]]
  50 bytes (5 words) - 13:49, 26 August 2020
• NADH-linked substrate state
  #REDIRECT [[NADH electron transfer-pathway state]]
  50 bytes (5 words) - 13:49, 26 August 2020
• NADH pathway control state
  #REDIRECT [[NADH electron transfer-pathway state]]
  50 bytes (5 words) - 13:50, 26 August 2020
• N-pathway
  #REDIRECT [[NADH electron transfer-pathway state]]
  50 bytes (5 words) - 13:50, 26 August 2020
• NADH-linked pathway
  #REDIRECT [[NADH electron transfer-pathway state]]
  50 bytes (5 words) - 13:51, 26 August 2020
• NADH pathway
  #REDIRECT [[NADH electron transfer-pathway state]]
  50 bytes (5 words) - 13:51, 26 August 2020
• NADH Electron transfer-pathway state
  #REDIRECT [[NADH electron transfer-pathway state]]
  50 bytes (5 words) - 08:10, 26 August 2020
• N-junction
  ...ning [[categories of SUIT protocols]] based on [[Electron-transfer-pathway state]]s. |info=[[N-pathway control state]]
  1 KB (143 words) - 14:20, 8 June 2020
• Glycerophosphate pathway control state
  |description=[[File:SUIT-catg_Gp.jpg|right|300px|Gp-pathway]] ...ced flavoprotein donates its reducing equivalents to the electron transfer-pathway at the level of [[CoQ]].
  2 KB (207 words) - 19:28, 25 August 2023
• Hydroxybutyrate
  ...te dehydrogenase produces acetoacetate while reducing NAD⁺ to [[NADH]].
  492 bytes (62 words) - 15:45, 2 August 2023
• Glycerophosphate shuttle
  ...epresenting a [[Electron-transfer-pathway state|ET pathway level 3 control state]].
  847 bytes (94 words) - 19:37, 1 January 2021
• GM-pathway control state
  '''MitoPathway control state:''' [[NADH electron transfer-pathway state]] The '''GM-pathway control state''' (glutamate-malate pathway control state) is established when glutamate&malate are added to isolated mitochondria, p
  2 KB (249 words) - 15:32, 2 August 2023
• NADH electron transfer-pathway state
  ...id]]). In the N-pathway control state [[Electron-transfer-pathway state|ET pathway level 4]] is active. |info=[[Electron-transfer-pathway state]], [[Gnaiger 2020 BEC MitoPathways]]
  3 KB (409 words) - 08:06, 25 November 2023
• F-junction
  ...in a reaction catalyzed by malate dehydrogenase to oxaloacetate (yielding NADH), which then stimulates the entry of AcetylCoA into the TCA cycle catalyzed
  1 KB (202 words) - 17:06, 18 January 2022
• N
  ::::» [[NADH electron transfer-pathway state]] N, N_''L'', N_''P'', N_''E''
  312 bytes (45 words) - 08:07, 6 July 2023
• Respiratory state
  ...f both, the [[coupling-control state]] and the [[electron-transfer-pathway state]]. ::::* [[OXPHOS]], [[LEAK respiration]], [[Electron transfer pathway|ET-pathway]] (''P, L, E'') - corrected for [[ROX]]. ''CCR'': ''[[L/E]]'', ''[[P/E]]'',
  3 KB (400 words) - 15:50, 15 August 2021
• PalOctPM
  '''MitoPathway control state:''' [[FN]] |mitopedia concept=SUIT state
  789 bytes (94 words) - 14:36, 26 August 2020
• PalOctPGMS
  '''MitoPathway control state:''' [[FNS]] |mitopedia concept=SUIT state
  852 bytes (97 words) - 14:36, 26 August 2020
• Hyphenation
  ...nt), but if the compound adjective follows the term (measurement at steady state), hyphenation does not add any information and should be avoided. Find more ...ate]] (''NOT'' 'Coupling control state' - it is not 'coupling of a control state')
  4 KB (469 words) - 02:31, 23 January 2023
• PalOctPGM
  '''MitoPathway control state:''' [[FN]] |mitopedia concept=SUIT state
  829 bytes (96 words) - 14:36, 26 August 2020
• NS e-input
  ...linked respiration. For more details, see: [[Additive effect of convergent electron flow]].
  1 KB (149 words) - 18:49, 1 January 2021
• Complex I-linked substrate state
  |description=''See'' '''[[N-pathway control state]]''' (previous: CI-linked) versus '''[[Complex I]]''' |mitopedia concept=Respiratory state, SUIT state, Find
  706 bytes (93 words) - 10:14, 2 November 2023
• PGM-pathway control state
  '''MitoPathway control state:''' [[NADH electron transfer-pathway state]] ...-oxoglutarate]]), representing the [[glutamate-anaplerotic pathway control state]]. 2-oxoglutarate (α-ketoglutarate) is formed from isocitrate (isocitrate
  3 KB (316 words) - 13:14, 2 August 2023
• Flavin adenine dinucleotide
  ...ion]] (compare [[N-junction]], [[Q-junction]]), in the [[F-pathway control state]]. In contrast, FADH₂ is not the substrate but the internal prod ::::* Gnaiger E (2023) Complex II ambiguities ― FADH2 in the electron transfer system. MitoFit Preprints 2023.3.v3. https://doi.org/10.26124/mitofit:2023-
  1 KB (199 words) - 18:57, 7 May 2023
• FN
  ...bation medium. FS in combination exerts an [[additive effect of convergent electron flow]] in most types of mitochondria. |info=[[Electron-transfer-pathway state]], [[Gnaiger 2020 BEC MitoPathways]]
  2 KB (311 words) - 19:57, 1 January 2021
• Electron transfer pathway
  |abbr=ET pathway ...-membrane, (2) [[TCA cycle]] and other mt-matrix dehydrogenases generating NADH and succinate, and (3) the carriers involved in metabolite transport across
  5 KB (646 words) - 01:06, 31 December 2020
• FNS
  ...ation medium. FNS in combination exerts an [[additive effect of convergent electron flow]] in most types of mitochondria. |info=[[Electron-transfer-pathway state]], [[Gnaiger 2020 BEC MitoPathways]]
  3 KB (403 words) - 19:58, 1 January 2021
• NS-S pathway control efficiency
  ..., S(Rot) or S. Then the NS-S pathway control efficiency in the ET-coupling state is ...s added to the NS-state. The reversed protocol, adding N-substrates to a S-pathway control background does not provide a valid estimation of S-respiration wit
  2 KB (288 words) - 18:30, 1 January 2021
• Complex I
  ...er mt-membrane, responsible for electron transfer to [[ubiquinone]] from [[NADH]] formed in the mt-matrix. CI forms a [[supercomplex]] with [[Complex III]] ...rnatively to a '''Complex I-linked substrate state''' ([[N-pathway control state]]).
  2 KB (214 words) - 13:43, 3 November 2023
• Cardoso 2022 Q10 Hamburg
  ...redox state and respiration in permeabilized HEK 293T cells: coupling and pathway control. Q10 Hamburg. ...rial inner membrane. ET capacity is measured in the noncoupled respiratory state after application of uncouplers, whereas LEAK respiration is assessed in th
  4 KB (580 words) - 18:34, 5 May 2022
• SUIT-020 O2 mt D032
  ...f the ATP synthase. Since higher concentrations of Omy can decrease the ET state induced upon addition of uncoupler, the required concentration of Omy has t :::* Comparison of GM- with PM-capacity yields important information on N-pathway respiratory control upstream of CI.
  4 KB (636 words) - 15:11, 17 September 2020
• Fatty acid oxidation pathway control state
  ...in a reaction catalyzed by malate dehydrogenase to oxaloacetate (yielding NADH), which then stimulates the entry of Acetyl-CoA into the TCA cycle catalyze |info=[[Electron-transfer-pathway state]], [[Gnaiger 2020 BEC MitoPathways]]
  3 KB (456 words) - 16:12, 13 January 2022
• NS-pathway control state
  |description=[[File:SUIT-catg NS.jpg|right|300px|NS-pathway control]] ...bation medium. NS in combination exerts an [[additive effect of convergent electron flow]] in most types of mitochondria.
  4 KB (604 words) - 08:32, 25 November 2023
• PM-pathway control state
  '''MitoPathway control state:''' [[NADH Electron transfer-pathway state]] ...is oxidatively decarboxylated to acetyl-CoA and CO₂, yielding [[NADH]] catalyzed by pyruvate dehydrogenase. [[Malate]] (M) is oxidized to oxaloa
  5 KB (603 words) - 15:33, 2 August 2023
• Categories of SUIT protocols
  ...inhibitors which define the [[Electron-transfer-pathway state]]s. The [[N-pathway control |N-type substrates]] are listed in parentheses, independent of the ...>2</sub>]]-linked substrates (FAO) with obligatory support by the N-linked pathway.
  5 KB (673 words) - 16:25, 3 June 2020
• Glutamate-anaplerotic pathway control state
  ...ised via mt-[[glutamate dehydrogenase]] in the mitochondrial matrix. The G-pathway plays an important role in [[glutaminolysis]]. LEAK state (''L'') with G alone as substrate can be evaluated in the following SUIT pr
  2 KB (276 words) - 20:11, 1 January 2021
• SUIT-007
  |abbr=[[Glutamate-anaplerotic pathway control state|Glutamate anaplerosis]] ...''' '''[[Glutamate-anaplerotic pathway control state|Glutamate anaplerotic pathway]]'''
  3 KB (401 words) - 11:30, 3 June 2020
• Moore 2017 MiPschool Obergurgl
  ...[[File:Moore Anthony L.jpg|left|90px|Anthony Moore]] The electron transfer-pathway – Q redox regulation and mitochondrial pathways to oxygen. ...favour of a homogenous Q-pool the redox state of which governs the rate of electron transport. I will also discuss deviations form Q-pool behaviour, its role w
  2 KB (223 words) - 12:48, 3 April 2021
• Cardoso 2023 MiP2023
  ...ransfer from beta-oxidation and TCA cycle and impact of OXPHOS coupling on NADH and coenzyme Q redox states. ...o analyze the relationships between the N- and Q-redox states and electron transfer rates. <br>
  4 KB (495 words) - 16:44, 12 July 2023
• Cooley 2001 J Bacteriol
  ...e the reduction level of NADP and NAD, which, in turn, affects respiratory electron flow through succinate dehydrogenase.
  2 KB (315 words) - 09:36, 20 April 2021
• Kaila 2014 Proc Natl Acad Sci U S A
  ...aila VR, Wikström M, Hummer G (2014) Electrostatics, hydration, and proton transfer dynamics in the membrane domain of respiratory Complex I. Proc Natl Acad Sc ...lecules establishing enhanced lateral connectivity and pathways for proton transfer between conserved ionizable residues along the center of the membrane domai
  2 KB (327 words) - 19:56, 27 January 2024
• Komlodi 2017 MiPschool Obergurgl
  ...and glycerophosphate-pathways to the Q-junction regulate reversed electron transfer to Complex I and H₂O₂ production. ...pressure generated by the linear S- and Gp-pathways or the convergent SGp-pathway on the Q-junction drives RET into CI and thus regulates H₂O<sub>
  5 KB (694 words) - 18:54, 10 January 2022
• SUIT-031
  ...are designed to measure simultaneously O₂ flux and the Q-redox state in isolated mitochondria and permeabilized cells. ...t D072]] simultaneous determination of O₂ flux and the Q-redox state in isolated mitochondria and cells permeabilized previously to addition to
  4 KB (623 words) - 17:57, 13 December 2023
• SUIT-021
  ...support NADH-linked respiration. Oligomycin (Omy) is used to induce a LEAK state of respiration via the inhibition of the ATP synthase. Since higher concent :::* Comparison of GM- with PM-capacity yields important information on N-pathway respiratory control upstream of CI.
  5 KB (697 words) - 15:58, 8 June 2020
• Schinagl 2016 PLoS One
  ...r W (2016) Adapting high-resolution respirometry to glucose-limited steady state mycelium of the filamentous fungus ''Penicillium ochrochloron'': method dev ...y does not depend on a complete saturation or inhibition of the cytochrome pathway.
  3 KB (386 words) - 15:46, 14 October 2023
• SUIT-020
  ...f the ATP synthase. Since higher concentrations of Omy can decrease the ET state induced upon addition of uncoupler, the required concentration of Omy has t :::* Comparison of GM- with PM-capacity yields important information on N-pathway respiratory control upstream of CI.
  5 KB (756 words) - 15:03, 17 September 2020
• Electron-transfer-pathway state
  |abbr=''ET-pathway state'' ...[[Categories of SUIT protocols]] are defined according to mitochondrial ET-pathway states.
  15 KB (1,913 words) - 19:27, 25 August 2023
• Alcázar-Fabra 2016 Biochim Biophys Acta
  ...ain components. These interactions would regulate mitochondrial CoQ steady-state levels and function. This article is part of a Special Issue entitled 'EBEC
  2 KB (234 words) - 16:11, 3 November 2021
• Nicotinamide adenine dinucleotide
  |abbr=NADH ...dition of NADH may indicate an alteration of the mtIM integrity. Cytosolic NADH is effectively made available for mitochondrial respiration through the [[m
  4 KB (477 words) - 10:41, 4 April 2024
• SUIT-012
  ...-[[ET capacity| ''E'']]) with NADH-linked substrates ([[PM-pathway control state|PM]] and PGM)''' ...may be relevant in the presence of [[glutamate-anaplerotic pathway control state]]. SUIT-012 can be extended with the CIV assay module.
  4 KB (505 words) - 23:57, 12 April 2021
• Van den Bergen 1994 Eur J Biochem
  ...den Bergen CW, Wagner AM, Krab K, Moore AL (1994) The relationship between electron flux and the redox poise of the quinone pool in plant mitochondria. Interpl ...oplasmic NADH ''in vivo'' uses the cyanide-resistant pathway more than the pathway involving the oxidation of succinate. A model is used to predict the kineti
  7 KB (1,023 words) - 09:03, 16 July 2021
• SUIT-021 Fluo mt D036
  ...f the ATP synthase. Since higher concentrations of Omy can decrease the ET state induced upon addition of uncoupler, the required concentration of Omy has t :::* Comparison of GM- with PM-capacity yields important information on N-pathway respiratory control upstream of CI.
  6 KB (834 words) - 14:07, 6 September 2021
• Komlodi 2021 MitoFit Q
  ...Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. https://doi.org/10.26124/mitofit: ...omlodi_2021_MitoFit_Q.pdf Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria]
  5 KB (767 words) - 08:41, 8 January 2023
• SUIT-021 O2 mt D035
  :::* Comparison of GM- with PM-capacity yields important information on N-pathway respiratory control upstream of CI. ...tate| S]] and [[NADH Electron transfer-pathway state| N-]] pathway control state.
  3 KB (420 words) - 09:20, 18 September 2020
• Cardoso 2022a EBEC
  ...rdoso LHD, Donnelly C, Komlódi T, Gnaiger E (2022) Characterizing electron transfer through the mitochondrial Q-junction from fatty acid oxidation and TCA cycl ...posite directions. Therefore, our aim was to characterize the influence of pathway and coupling control on these two bioenergetic parameters.
  4 KB (519 words) - 14:25, 23 August 2022
• SUIT-012 O2 mt D027
  |info='''A''' Linear coupling control with NADH-linked substrates for isolated mitochondria, tissue homogenate and permeabi ...he involvement of the [[complex II]] ([[Succinate pathway control state| S-pathway]]). It can be useful to understand the contribution and the activity of the
  3 KB (397 words) - 00:12, 13 April 2021
• Cardoso 2022 Abstract Bioblast
  ...nitoring of respiration together with NAD(P)H autofluorescence and Q-redox state [1] provides unique analytical and diagnostic power in the study of mitocho ...Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. https://doi.org/10.26124/bec:2021
  3 KB (463 words) - 08:34, 28 July 2022
• SUIT-009 AmR mt D021
  ...K state. Pyruvate (P) supports [[NADH Electron transfer-pathway state|NADH-pathway (N)]], and usually in the presence of ADP it does not increase further the :::: 1. the S- and NS-pathway-linked respiration and H₂O₂ flux.
  5 KB (681 words) - 00:11, 13 April 2021
• Residual endogenous substrates
  ...sence of ADP to stimulate the consumption of endogenous fuel substrates: [[State 2]]). ''Ren'' values may be used as technical replicates when obtained from ...gly, Q and NAD are not fully oxidized in the REN state compared to the ROX state.
  2 KB (325 words) - 08:35, 25 November 2023
• Succinate pathway
  ...see [[Gnaiger 2009 Int J Biochem Cell Biol]]). The S-pathway describes the electron flux through [[Complex II]] (CII; see [[succinate dehydrogenase]], SDH) fro The S-pathway control state is usually induced in mt-preparations by addition of succinate&rotenone. In
  8 KB (1,053 words) - 08:04, 30 March 2023
• Cardoso 2022 ESCI Bari
  ...getic fingerprinting with coupling and pathway control of coenzyme Q redox state and respiration in permeabilized HEK 293T cells. 56^th Annual Sci ...iagnostic fingerprinting is challenged with targeting specific segments of pathway and coupling control of oxidative phosphorylation (OXPHOS) [1].
  4 KB (520 words) - 14:41, 30 May 2022
• Nemec 2018 MiP2018
  ...e (n=10) in seniors (28F/6M; age 66.5±4.3; BMI 26.4±4.0) on the functional state of the skeletal muscle mitochondria. ...(FNS_E) fueled by electrons from octanoyl oxidation, NADH-linked pathway and succinate, effects of stretching exercise were less pronounced (FNS<sub
  4 KB (476 words) - 00:16, 18 March 2019
• Bioblast alert 2016
  ...age, if we say ‘Complex I’ – when actually having in mind the pathway from NADH-linked substrates to CI - CIII - CIV. Should we rather say CI-CIII-CIV-link ...'are the two enzyme complexes added?''), when we actually measure electron transfer linked to CI<small>&</small>II - CIII - CIV !!!
  4 KB (529 words) - 09:59, 3 June 2020
• Komlodi 2021 BEC Q
  ...Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioenerg Commun 2021.3. https://d ...ransfer system, reflect the balance between (''1'') reducing capacities of electron flow from fuel substrates converging at the Q-junction, (''2'') oxidative c
  6 KB (810 words) - 16:37, 21 January 2024
• Komlodi 2022 Q10 Hamburg
  ...s: Continuous monitoring of pull and push control of respiration and redox state of the Q-mimetic CoQ₂. Q10 Hamburg. ...mplemented into the Oroboros NextGen-O2k to monitor continuously the redox state of CoQ2 added as a Q-mimetic simultaneously with O2 consumption (Komlódi e
  4 KB (559 words) - 18:39, 5 May 2022
• Briston 2017 Sci Rep
  ...nt with inhibition of complex I-supported respiration by leakage of matrix NADH. Additionally, ROS generated at complex III did not sensitise mitochondria
  2 KB (324 words) - 12:54, 10 March 2020
• Q-pools
  ...ting in the Q_hom-pool, whereas the CI-Q-CIII segment of the [[N-pathway]] indicates [[supercomplex]] organization and metabolic channeling with dif | ETS-reactive Q || Q_ra = Q || electron-transfer-system reactive mtCoQ, fully reduced under anoxia, fully oxidized in aerobi
  5 KB (698 words) - 21:19, 3 April 2021
• SUIT-028
  ! Respiratory state ! Pathway control
  4 KB (574 words) - 16:13, 14 April 2020
• Chicco 2022 Abstract Bioblast
  ...''J''_O₂, revealing a complex interaction of N- and S-pathway substrate oxidation on ''J''_O₂. ...way flux in SUIT protocols used to study the additive effect of convergent electron flow at the Q-junction.
  4 KB (625 words) - 08:33, 28 July 2022
• SUIT-006
  ...tate appropriately. Since higher concentrations of Omy can decrease the ET state induced upon addition of uncoupler, the required concentration of Omy has t ...tential measurements, choose [[SUIT-006 Fluo mt D034]]. For O2 and Q redox state measurements, choose [[SUIT-006 Q mt D071]] or [[SUIT-006 Q ce-pce D073]].
  8 KB (1,169 words) - 13:08, 13 December 2023
• SUIT-009 AmR ce-pce D019
  ...Electron transfer-pathway state|NADH-pathway]] and thus the convergent NS-pathway with little further effect on H₂O₂ flux, NS<sub>''P'' ...Information is obtained on respiration and ROS production in the S- and NS-pathway.
  5 KB (832 words) - 00:10, 13 April 2021
• Malate
  ...capacity of the FAO-pathway can be overestimated due to a contribution of NADH-linked respiration, F(N) (see [[SUIT-002]]). ...H and S (NS-) pathway, whereas 0.5 mM malate was apparently saturating for NADH (N-) linked respiration. However, this is not true in the presence of [[mal
  4 KB (585 words) - 19:57, 5 February 2022
• Muller 2008 Biochem J
  ...Complex II. In summary, the present results indicate that reverse-electron transfer-mediated superoxide production can occur under physiologically realistic su
  3 KB (401 words) - 17:48, 16 January 2021
• SUIT-020 Fluo mt D033
  :::* Comparison of GM- with PM-capacity yields important information on N-pathway respiratory control upstream of CI. ...sed [[Mitochondrial membrane potential|mt-membrane potential]] in the LEAK state.
  5 KB (655 words) - 08:41, 30 September 2020
• SUIT-009
  ...AK state. Pyruvate (P) promotes[[NADH Electron transfer-pathway state|NADH-pathway (N)]], and usually in the presence of ADP, H₂O₂ flux ...d to a decrease in the N-linked respiration and a reduced reverse electron transfer-supported ROS production.
  6 KB (864 words) - 23:56, 12 April 2021
• Gnaiger 2009 Int J Biochem Cell Biol
  ...ht healthy adults with predominantly sedentary life style. The apparent ET-pathway excess capacity (noncoupled respiration) over ADP-stimulated OXPHOS capacit :::* '''2017''': For further clarification, NADH-linked respiration (N-respiration; CI-linked) and succinate-linked respirat
  7 KB (975 words) - 13:46, 1 May 2024
• Baglivo 2023 ESCI Prague
  ...he redox link between Complexes CIII and CIV of the mitochondrial electron transfer system. Cytochrome c release across the mitochondrial outer membrane (mtOM) ...steps in RP1 use pyruvate & malate to analyze coupling control in the NADH-pathway. RP2 focuses on fatty acid oxidation (FAO), considering malate-linked anapl
  3 KB (413 words) - 14:32, 9 May 2023
• Gnaiger 2017 Abstract MitoEAGLE Barcelona
  ...HOS-capacity. This would be obscured when considerig the electron transfer-pathway (Fig. 1) or ET-capacity as being primarily controlled by the membrane-bound [[File:ETS and NS-SUIT protocols.jpg|left|450px |ET-pathway and NS-SUIT protocols]]
  7 KB (927 words) - 18:53, 10 January 2022
• Ojuka 2015 Abstract MiPschool Cape Town 2015
  dehydrogenases transfer single electrons to electron transferring dehydrogenase transfers electrons to NAD+ and the reduced NADH
  3 KB (438 words) - 16:16, 26 March 2018
• Doerrier 2017 MiPschool C2
  ...ous studies demonstrate [6] that N pathway cannot be fully isolated from S pathway. S-contribution in SUIT protocols depends on the N-substrate combinations u ...-cytochrome c oxidoreductase) and CIV (cytochrome c oxidase), to the final electron acceptor oxygen.
  5 KB (706 words) - 12:00, 27 May 2019
• Wollenman 2017 PLOS ONE
  ...f 16%, 26%, and 35% for the leak state, ADP-simulated state, and uncoupled state, respectively, relative to rates using B1. The common feature distinguishin ...ol state |Pyruvate&malate, PM]]: [[NADH electron transfer-pathway state |N-pathway]]
  10 KB (1,407 words) - 19:20, 9 February 2022
• Cytochrome c
  ...''''' is a component of the Electron transfer-pathway ([[Electron transfer pathway]]) in mitochondria. It is a small heme protein loosely associated with the ...tochrome ''c'' addition (2c) in OXPHOS state, using pyruvate and malate as NADH-linked substrates. An increase in oxygen flux per volume (red trace, right
  6 KB (844 words) - 09:37, 4 April 2024
• Gnaiger 2018 MiPschool Tromso A1
  ....jpg|left|90px|Erich Gnaiger]] Mitochondrial states and rates: 1. Electron transfer pathways and respiratory control. 2. Coupling control. ...d SUIT protocols reveal a diversity of mt-respiratory control patterns and pathway additivity depending on species, organs, cell types, and pathophysiological
  10 KB (1,441 words) - 23:46, 25 January 2021
• Fatty acid oxidation
  ...hereas NADH is the substrate of CI, FADH₂ is the substrate of [[electron-transferring flavoprotein complex]] (CETF) which is localized on the matrix ...h oxaloacetate (CS reaction). Therefore, FAO implies simultaneous electron transfer into the [[Q-junction]] through CETF and CI.
  4 KB (596 words) - 09:27, 8 May 2023
• Doerrier 2017 Abstract MitoEAGLE Barcelona
  ...ous studies demonstrate [6] that N pathway cannot be fully isolated from S pathway. S-contribution in SUIT protocols depends on the N-substrate combinations u ...-cytochrome c oxidoreductase) and CIV (cytochrome c oxidase), to the final electron acceptor oxygen.
  6 KB (754 words) - 12:00, 27 May 2019
• SUIT-006 AmR mt D048
  ...e chamber) in a wide variety of species and tissues. After addition of the NADH-linked substrates, the addition of ADP depolarises the mt-membrane which le [[Oligomycin]] (Omy) is used to induce a LEAK state of respiration via the inhibition of the ATP synthase. Omy hyperpolarises t
  4 KB (615 words) - 23:51, 12 April 2021
• Miller 2017 PLOS ONE
  ...2017) Mitochondrial respiration in highly aerobic canines in the non-raced state and after a 1600-km sled dog race. PLOS ONE 12:e0174874. ...way (ET-pathway; non-coupled, E). After racing respiratory capacities from NADH-linked substrates, but not fat-derived substrates increased. Finally, the O
  3 KB (438 words) - 12:52, 27 December 2021
• Doerrier 2018 Methods Mol Biol
  ...l upstream of the Q-cycle, providing diagnostic information on specific ET-pathway branches. O2 concentration is maintained above air saturation in protocols ...2021 AmR-O2, MitoFit 2021 AmR, MitoFit 2021 Tissue normoxia, MitoFit 2022 NADH, MitoFit 2021 BCA, MitoFit 2021 PLT, PLoSONE2022ace-sce, MitoFit2022QC
  6 KB (850 words) - 12:27, 5 March 2024
• SUIT-006 Fluo mt D034
  ...h fluorescent dyes. In this protocol, the [[NADH Electron transfer-pathway state]] can be analyzed in [[Mitochondrial preparations| mitochondrial preparatio ...sed [[Mitochondrial membrane potential|mt-membrane potential]] in the LEAK state.
  4 KB (623 words) - 14:08, 6 September 2021
• Gnaiger Abstract MiP2010 2-01
  ...for measuring the upper limits of OXPHOS and Electron transfer-pathway (ET-pathway) capacity. Estimates of maximum mitochondrial performance (2) provide essen ...for measuring the upper limits of OXPHOS and Electron transfer-pathway (ET-pathway) capacity. Estimates of maximum mitochondrial performance (2) provide essen
  8 KB (1,121 words) - 19:16, 10 January 2022
• Residual oxygen consumption
  ...distinguished from the corresponding ''Rox''-corrected, mitochondrial (ET-pathway linked) fluxes: ''R''(mt), ''L''(mt), ''P''(mt) and ''E''(mt). Alternative ...inguished from the ROX state. However, in novel applications (Q-Module and NADH-Module), a distinction of these states is necessary. Care must be taken whe
  10 KB (1,409 words) - 08:36, 25 November 2023
• Komlodi 2017 Abstract MITOEAGLE Barcelona
  ...egulation of respiration and hydrogen peroxide production in the succinate pathway in view of an ADP-induced respiratory depression observed in some tissues. ...trically different for S(-Rot) (decrease of NADH) and S(+Rot) (increase of NADH) in mouse cardiac tissue.
  6 KB (912 words) - 18:51, 10 January 2022
• Lemieux 2019a MiP2019
  ...en a great amount of research on the clinical observations and biochemical pathway activations of PBM (i.e. secondary effects), the direct changes to mitochon ...inate pathway (with succinate providing electrons into complex II), the NS-pathway (with complex I- and II-linked substrates simultaneously), and the complex
  6 KB (903 words) - 13:12, 26 September 2019
• Pesta 2012 Methods Mol Biol
  ...substrates for fatty acid oxidation (FAO), NADH-pathway (N), and succinate-pathway (S). Superimposed oxygraph traces (mass-specific oxygen flux [pmol O2∙s<s ...iding diagnostic information on specific branches of the Electron transfer pathway. Oxygen levels are maintained above air saturation in protocols with permea
  8 KB (1,056 words) - 10:11, 23 May 2022
• Garcia-Roves 2022 Abstract Bioblast
  ...>Garcia-Roves Pablo M</u> (2022) Tuning the assessment of coenzyme Q redox state and respiration in permeabilized skeletal muscle fibers. '''Bioblast 2022: ...vant information about the function of specific components of the electron transfer system. In addition, the growing popularization of this methodology has bee
  6 KB (821 words) - 08:35, 28 July 2022
• Silva 2019 MiPschool Coimbra
  ...esence of anaplerotic reactions [F(N)] in different electron transfer (ET) pathway states. Substrate-uncoupler-inhibitor titration protocol was used to study ...selectively blocked by etomoxir without inhibiting other mitochondrial ET-pathway states on permeabilized Huh7 cells and isolated liver mitochondria. In agre
  7 KB (977 words) - 19:13, 10 January 2022
• Sobotka 2017 MiP2017
  ...After six weeks and longer, however, the total mitochondrial capacity for NADH-linked substrates and for succinate was inhibited. ...during NAFLD progression. However, the total capacity of electron transfer-pathway of fatty liver mitochondria increased and consequently decreased after 3 an
  3 KB (470 words) - 11:03, 18 March 2020
• MitoPediaAlert2020 Archive
  |''' What is the limitation of State 3 respiration compared to [[OXPHOS capacity]]? '''<br> ...tration of ADP, P_i, fuel substrates and O₂. However, State 3 as defined by Chance and Williams (1955) can lead to underestimation of O
  21 KB (2,889 words) - 13:31, 19 January 2021
• The protonmotive force and respiratory control
  ...bbreviations (mtDNA, mt to abbreviate mitochondr*); OXPHOS capacity versus State 3 (discuss saturating ADP/Pi .. concentrations). ::::* '''[[Electron transfer pathway]]'''
  12 KB (1,564 words) - 23:40, 25 January 2021
• Krumschnabel 2014 Methods Enzymol
  ...mandatory for any fluorophore applied for probing mtMP, in any respiratory state, type of tissue and pathophysiological condition. ...ntial, Safranin, Complex I, Complex II, OXPHOS analysis, electron transfer-pathway
  5 KB (600 words) - 21:53, 23 April 2024
• Silva 2019 ESCI2019
  ...mitochondrial side-effects of etomoxir at different electron transfer (ET) pathway states. Substrate-uncoupler-inhibitor titration protocol was used to study ...he anaplerotic pathways [5]), etomoxir did not influence FAO in the OXPHOS state on Huh7 cells, while on mitochondria respiring on palmitoyl-CoA (40 µM) +
  5 KB (697 words) - 19:11, 10 January 2022
• Chabi 2019 MiP2019
  ...te (NS-OXPHOS capacity). 5U: uncoupler titrations to evaluate the electron transfer- (ET-) capacity (NS-ET capacity). 6Rot: inhibition of CI by rotenone (S-ET ...ntrol of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. [[SUIT
  7 KB (971 words) - 12:55, 21 February 2023
• Chabi 2019 MitoFit Preprint Arch EA
  ...OXPHOS capacity). :'''5U:''' uncoupler titrations to evaluate the electron transfer- (ET-) capacity (NS-ET capacity). '''6Rot:''' inhibition of CI by rotenone ...ntrol of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840, DOI:10
  8 KB (1,064 words) - 08:25, 8 January 2023
• Doerrier 2019 MitoFit Preprint Arch EA
  ...''_O2,PM)/''I''_O2,''c''PM, exceeded 0.1 in the OXPHOS-state (Fig. 1; steps 2D and 2c). For abbreviations see Figure 1 and Gnaiger et al ...-OXPHOS capacity). '''5U''': uncoupler titrations to evaluate the electron transfer- (ET-) capacity (NS-ET capacity). '''6Rot''': inhibition of CI by rotenone
  9 KB (1,242 words) - 08:27, 8 January 2023
• Bioblast quiz
  ...''The protonmotive force and respiratory control. 1. Coupling of electron transfer reactions to vectorial translocation of protons. 2. From Einstein’s diffu || If we want to relate the driving force of electron transfer to the '''pmf''', we have to express both forces in identical [[motive unit
  36 KB (5,157 words) - 12:10, 12 April 2024
• IOC recommended reading
  ...ntrol of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840, DOI:10 ...ys (CI-entry into Q); succinate-linked or S-pathway (CII-entry into Q); NS-pathway (convergent CI<small>&</small>II-entry into Q).
  12 KB (1,521 words) - 14:23, 15 May 2023
• Iyer 2022 Abstract Bioblast
  ...ygen consumption ''Rox'' after inhibition by rotenone and antimycin A (ROX state), and LEAK respiration ''L''. The glycoBHI was based on four key aspects of ...ATP synthase and low heteroplasmy was detected in disease lines affecting NADH dehydrogenase; (''2'') levels of defective enzyme activities of the ETS cor
  6 KB (863 words) - 16:22, 20 June 2022
• O2k-Publications: Topics
  | style="text-align:left"|[[O2k-Publications: Redox state |Redox state]] ...egory:Publications]] [[Instrument and method::Oxygraph-2k]] [[Topic::Redox state]] |format=count}}
  62 KB (7,038 words) - 14:31, 22 August 2023
• Oroboros Ecosystem Agenda 2024 Archive
  ...is simultaneous electron transfer from FAO into the Q-junction through the electron transferring flavoprotein dehydrogenase complex (CETFDH) and Complex I. <br ...ects on oxidative phosphorylation, mitochondrial membrane potential, redox state of coenzyme Q, and calcium uptake]]''
  27 KB (4,003 words) - 08:47, 6 May 2024
• Complex II ambiguities
  ...ehydrogenase (CGpDH) feeds electrons into the ETS through CII. In reality, NADH and succinate produced in the TCA cycle are the substrates of Complexes CI ...Gnaiger E (2024) Complex II ambiguities ― FADH₂ in the electron transfer system. J Biol Chem 300: 105470. https://doi.org/10.1016/j.jbc.2023.105470
  16 KB (2,064 words) - 08:21, 22 March 2024
• Gnaiger 2012 MitoPathways
  ...e electron transfer-pathway. XLII. Reconstitution of the electron transfer-pathway. J Biol Chem 237:2661-9.]] ...e | Mitchell P (1961) Coupling of phosphorylation to electron and hydrogen transfer by a chemi-osmotic type of mechanism. Nature 191:144-8.]]
  34 KB (4,820 words) - 04:08, 23 November 2021
• MiPNet28.02 IOC162 Schroecken AT
  ...ate-Uncoupler-Inhibitor-Titration (SUIT) protocols: exploring coupling and pathway control] ...3-10-06_Grings_IOC_162_Q_NADH_PB.pdf The NextGen O2k all-in-one: Q-Module, NADH-Module, and PhotoBiology-Module]
  14 KB (2,037 words) - 16:11, 2 November 2023
• MitoEAGLE Task Group States and rates
  ...ET; proton leak, LEAK, residual oxygen consumption, ROX, State 2, State 3, State 4, normalization, flow, flux, O₂ ...(electron donors) and reduction of O₂ catalysed by the electron transfer system, ETS: ('''mt''') mitochondrial catabolic respiration; ('''ce''') tot
  34 KB (4,911 words) - 11:25, 1 September 2021
• Safranin
  ...587 nm. Safranin is a potent inhibitor of [[NADH electron transfer-pathway state |N-linked respiration]] and of the [[phosphorylation system]].
  13 KB (1,842 words) - 17:09, 17 January 2024
• Gnaiger 2014 MitoPathways
  ...e electron transfer-pathway. XLII. Reconstitution of the electron transfer-pathway. J Biol Chem 237:2661-9. - [[Hatefi 1962 J Biol Chem |»Bioblast link«]] :::# Mitchell P (1961) Coupling of phosphorylation to electron and hydrogen transfer by a chemi-osmotic type of mechanism. Nature 191:144-8. - [[Mitchell 1961 N
  51 KB (7,005 words) - 04:36, 19 July 2022
• Gnaiger 2012 MitoPathways References
  # Brandt U (2006) Energy converting NADH:quinone oxidoreductase (Complex I). Annu Rev Biochem 75:69-92. ...ureau Mirand P, Boirie Y, Morio B, Mosoni L (2005) Due to reverse electron transfer, mitochondrial H2O2 release increases with age in human vastus lateralis mu
  25 KB (3,492 words) - 06:44, 13 January 2021
• Gnaiger 2020 BEC MitoPathways
  It is now our responsibility to transfer the enthusiasm for innovation, reproducibility, and quality in science, and ...hosphorylation (OXPHOS) determined in mitochondrial preparations, electron transfer (ET) capacity, LEAK respiration, and the distinction of uncoupled, noncoupl
  89 KB (12,074 words) - 17:54, 6 May 2024
• Oroboros Ecosystem Agenda 2021 Archive
  |'''Coenzyme Q - a mobile redox compound in the electron transfer system '''<br> ...cells. In humans, CoQ₁₀ occurring in the mitochondrial electron transfer system transfers electrons from dehydrogenases and respiratory complexes do
  85 KB (11,819 words) - 14:18, 10 January 2022
• Oroboros Ecosystem Agenda 2023 Archive
  * Gnaiger E (2023) Complex II ambiguities ― FADH2 in the electron transfer system. [[Gnaiger 2023 J Biol Chem|»Bioblast link«]] ...in different respiratory states to the maximum flux in a common reference state. <br>
  80 KB (11,165 words) - 09:37, 8 January 2024
• MitoFit Open Seminars
  ...ion of ATP production: dependence on calcium concentration and respiratory state", [[Fink 2017 Am J Physiol Cell Physiol]] ...T| Timea Komlodi]]: Membrane potential and delta pH dependency of reverse electron transport-associated hydrogen peroxide production in brain and heart mitoch
  33 KB (4,250 words) - 15:07, 2 February 2022
• Picard 2011 PLoS One
  ...h greater magnitude. Furthermore, mitochondrial isolation markedly altered electron transport chain protein stoichiometry. Collectively, our results demonstrat ...o (and should read 10 mM succinate) or is ineffective in stimulating the S-pathway.
  52 KB (7,111 words) - 14:50, 13 January 2023
• Short-Term Scientific Missions MitoEAGLE
  | Simultaneous measurements of oxygen, mitochondrial membrane potential and NADH using the O2k-NextGen ...te cell activation and differentiation modulated by the calcineurin/NFATc2 pathway: Implications of cellular bioenergetics, respiration and autophagy fluxes
  41 KB (5,420 words) - 15:06, 23 November 2020
• Oroboros Ecosystem Agenda 2020 Archive
  Application of safranin leads to partial inhibition of NADH-linked respiration and above 2 µM on the succinate-linked respiration. By ...embrane potential: A method to detect deficiency in mitochondrial electron transfer system''' <br>
  126 KB (17,785 words) - 08:55, 13 August 2021
• Oroboros Ecosystem Agenda 2022 Archive
  |'''"Our results suggest increased proton and electron leak and a decreased ETS capacity in platelets in chronic smoke-exposed ani ...ey metabolic changes supporting the major importance of an NAD⁺/NADH imbalance in PMD." '''<br>
  87 KB (11,941 words) - 15:14, 16 November 2023
• BEC 2020.1 doi10.26124bec2020-0001.v1
  ...• [[Count |Cell count]] • [[Coupling-control ratio]] • [[Electron transfer pathway]] • [[Flow]] • [[Flux]] • [[Flux control ratio]] • [[IUPAC]] • [[ ...(1955b) Respiratory enzymes in oxidative phosphorylation: III. The steady state. J Biol Chem 217:409-27. - [[Chance 1955 J Biol Chem-III |»Bioblast link«
  59 KB (8,156 words) - 13:41, 1 May 2024
• Gnaiger 2024 J Biol Chem
  ...Gnaiger E (2024) Complex II ambiguities ― FADH₂ in the electron transfer system. J Biol Chem 300:105470. https://doi.org/10.1016/j.jbc.2023.105470 ...lic acid cycle. However, several graphical representations of the electron transfer system depict FADH₂ in the mitochondrial matrix as a substrate t
  135 KB (18,142 words) - 13:29, 1 May 2024