Difference between revisions of "SUIT-017 O2 pfi D049"
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::::+ Glutamate is easier to prepare compared to pyruvate. | ::::+ Glutamate is easier to prepare compared to pyruvate. | ||
::::+ Reasonable duration of the experiment. | ::::+ Reasonable duration of the experiment. | ||
::::+ This protocol can be extended with the Complex IV module, which can prolong the experimental time with ~30 min. | |||
== Compare SUIT protocols == | == Compare SUIT protocols == | ||
::::* [[SUIT-002]] | ::::* [[SUIT-002]] |
Revision as of 11:22, 7 March 2019
Description
Abbreviation: FNS(Oct,GM)
Reference: A: SUIT-017
SUIT number: D049_1OctM;2D;2c;3G;4S;5U;6Rot;7Ama
O2k-Application: O2
- MitoPedia: SUIT
- SUIT-category: FNS(Oct,GM)
- SUIT protocol pattern: diametral 1OctM;2D;2c;3G;4S;5U;6Rot-
Communicated by Cardoso LH and Gnaiger E (last update 2019-02-26)
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1OctM | OctML(n) | F(N) | FAO | 1OctM
|
2D | OctMP | F(N) | FAO | 1OctM;2D
|
3G | OctGMP | FN | F&CI | 1OctM;2D;3G
|
4S | OctGMSP | FNS | F&CI&II | 1OctM;2D;3G;4S
|
5U | OctGMSE | FNS | F&CI&II | 1OctM;2D;3G;4S;5U
|
6Rot | SE | S | CII | 1OctM;2D;3G;4S;5U;6Rot
|
7Ama | ROX | 1OctM;2D;3G;4S;5U;6Rot;7Ama
|
Step | Respiratory state | Pathway control | ET-Complex | Comment |
---|---|---|---|---|
## AsTm | AsTmE | CIV | CIV | |
## Azd | CHB |
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- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- Comparison of GM- with PM-capacity yields important information on N-pathway respiratory control upstream of CI (Lemieux et al. 2017; Votion et al. 2012).
- + Glutamate is easier to prepare compared to pyruvate.
- + Reasonable duration of the experiment.
- + This protocol can be extended with the Complex IV module, which can prolong the experimental time with ~30 min.
Compare SUIT protocols
References
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
Pesta 2012 Methods Mol Biol | 2012 | Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_3 | Human | Skeletal muscle Other cell lines HEK Fibroblast HUVEC |
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry