Noncoupled respiration is distinguished from general (pharmacological or mechanical) uncoupled respiration, to give a label to an effort to reach the state of maximum uncoupler-activated respiration without inhibiting respiration. Noncoupled respiration, therefore, yields an estimate of ET-capacity. Experimentally uncoupled respiration may fail to yield an estimate of ET-capacity, due to inhibition of respiration above optimum uncoupler concentrations or insufficient stimulation by sub-optimal uncoupler concentrations. Optimum uncoupler concentrations for evaluation of (noncoupled) ET-capacity require inhibitor titrations (Steinlechner-Maran 1996 Am J Physiol Cell Physiol; Huetter 2004 Biochem J; Gnaiger 2008 POS).
MitoPedia methods: Respirometry
MitoPedia topics: EAGLE
Contributed by Gnaiger E 2010-08-15, edited 2015-01-19.
Is respiration uncoupled - noncoupled - dyscoupled?
- » More details: Uncoupler
Biochemical coupling efficiency: from 0 to <1
Related MitoPedia pages
- Electron transfer-pathway states
- Coupling control state E
- » Noncoupled respiration
- » Is respiration uncoupled - noncoupled - dyscoupled?