MitoPedia: SUIT A

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MitoPedia

MitoPedia: SUIT A

MitoPedia - high-resolution terminology - matching measurements at high-resolution.
The MitoPedia terminology is developed continuously in the spirit of Gentle Science.

MitoFit
In the Library of SUIT protocols
» MitoPedia: SUIT - SUIT concept and a list of all SUIT protocols in the library of SUIT protocols.
»A: MitoPedia: SUIT A - you are here: these are SUIT protocols used for cohort studies.
»B: MitoPedia: SUIT B - these are published SUIT protocols considered less suitable for cohort studies.
»C: MitoPedia: SUIT C - these are SUIT protocols used for exploratory research.
See also:
» O2k-Protocols: O2k-Demo experiments



Library of SUIT protocols: SUIT A

SUIT codeSUIT numberSUIT categoryCoupling/pathway control diagramO2k-ApplicationReference
SUIT-001 O2 ce-pce D003D003_ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11AzdRP1 ce-pceSUIT-RP1O2A - SUIT-001
SUIT-001 O2 ce-pce D004D004_ce1;1Dig;1PM;2D;2c;3U;4G;5S;(6Oct);7Rot;8Gp;9Ama;10AsTm;11AzdRP1 ce-pce bloodSUIT-RP1 for PBMCs and PLTsO2A - SUIT-001
SUIT-001 O2 mt D001D001_1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11AzdRP1 mtSUIT-RP1O2SUIT-001 - SUIT reference protocol Reference protocol RP1 for isolated mitochondria, tissue homogenate and permeabilized cells
SUIT-001 O2 pfi D002D002_1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11AzdRP1 pfiSUIT-RP1O2SUIT-001
SUIT-002 O2 ce-pce D007D007_ce1;1Dig;1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13AzdRP2 ce-pceSUIT-RP2O2A - SUIT-002
SUIT-002 O2 ce-pce D007aD007a_ce1;1Dig;1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13AzdRP2 ce-pce bloodSUIT-RP2 for PBMCs and PLTsO2A - SUIT-002
SUIT-002 O2 mt D005D005_1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13AzdRP2 mt1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd.pngO2A - SUIT-002
SUIT-002 O2 pfi D006D006_1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13AzdRP2 pfiSUIT-RP2O2A -SUIT-002
SUIT-003 AmR ce D058D058_ce1;ce1SOD;ce1HRP;ce1AmR;ce2Omy;ce3U;ce4Rot;ce5AmaAmR effect on ceCe1;ce1SOD;ce1HRP;ce1AmR;ce2Omy;ce3U;ce4Rot;ce5Ama.pngAmRB: Coupling control in intact cells with oligomycin to study the inhibitory effect of the AmR assay components on the respiration ; ce: non-permeabilized cells -SUIT-003
SUIT-003 O2 ce D009D009_ce1;ce2Omy;ce3U;ce4Rot;ce5AmaCCP-ce shortCe1;ce2Omy;ce3U-.pngO2A: - SUIT-003
SUIT-003 O2 ce D037D037_ce1;ce1Glc;(ce2Omy);ce3U;ce4AmaCCP-ce Crabtree_RCe1;ce1Glc;ce2(Omy);ce3U;ce4Ama.pngO2A Coupling control protocol and Crabtree effect - SUIT-003
SUIT-003 O2 ce D038D038_ce1;(ce2Omy);ce3U;ce3Glc;ce3'U;ce4AmaCCP-ce Crabtree_ECe1;ce2(Omy);ce3U;ce3Glc;ce3'U;ce4Ama.pngO2A Coupling control protocol and Crabtree effect test -SUIT-003
SUIT-003 O2 ce D039D039_ce1;(ce2Omy);ce3U;ce4Rot;ce5AmaCCP-ce microalgaeCe1;(ce2Omy);ce3U;ce4Rot;ce5Ama.jpgO2A: Coupling control in intact cells without oligomycin; ce: non-permeabilized cells -SUIT-003
SUIT-003 O2 ce D050D050_ce1;ce1Snv;(ce2Omy);ce3U;ce4Rot;ce5AmaCCP-ce SnvCe1;ce1Snv;(ce2Omy);ce3U;ce4Rot;ce5Ama.pngO2A: - Coupling control protocol for living cells with plasma membrane-permeable succinate (MitoKit-CII/Succinate-nv) - SUIT-003
SUIT-003 O2 ce-pce D020D020_ce1;ce1P;ce2Omy;ce3U;ce4Rot;ce5S;1Dig;1c;2Ama;3AsTm;4AzdCCVPCe1;ce1P;ce2Omy;ce3U;ce4Rot;ce5S;1Dig;1c;2Ama;3AsTm;4Azd.pngO2A Coupling control protocol and respirometric cell viability test; ce: living cells, pce: permeabilized cells - SUIT-003
SUIT-004 O2 pfi D010D010_1PM;2D;2c;3U;4S;5Rot;6Ama;7AsTm;8AzdRP1-short pfi1PM;2D;2c;3U;4S;5Rot;6Ama;7AsTm;8Azd.pngO2SUIT-004
SUIT-005 O2 pfi D011D011_1OctM;2D;3P;4S;5U;6Rot;7Ama;8AsTm;9AzdRP2-short pfi1OctM;2D;2c;3P;4S;5U;6Rot;7Ama;8AsTm;9Azd.pngO2A: - SUIT-005
SUIT-006 AmR mt D048D048_1PM;2D;3Omy;4U;5AmaCCP mt PM - AmR1PM;2D;3Omy;4U;5Ama.pngAmRA: short protocol for simultaneous determination of O2 flux and H2O2 in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)- SUIT-006
SUIT-006 Fluo mt D034D034_1PM;2D;3Omy;4U;5AmaCCP mt PM - Fluo1PM;2D;3Omy;4U;5Ama.pngFluoA: short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) -SUIT-006
SUIT-006 MgG mt D055D055_1PM;2D;3Cat;4U;5AmaCCP mt PM - MgG1PM;2D;3Cat;4U;5Ama.pngMgGA: short coupling control protocol for simultaneous determination of O2 flux and mitochondrial ATP production in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) -SUIT-006
SUIT-006 O2 ce-pce D029D029_ce1;1Dig;1PM;2D;2c;3Omy;4U;5AmaCCP ce-pce PMCe1;1Dig;1PM;2D;2c;3Omy;4U;5Ama.pngO2A to analyse the coupling control on permeabilized cells in N(PM) pathway control state- SUIT-006
SUIT-006 O2 mt D022D022_1SRot;2D;2c;(3Omy);4U;5AmaCCP mt S(Rot)1SRot;2D;2c;3(Omy);4U;5Ama.pngO2A - SUIT-006 - Protocol for coupling control assessment in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)
SUIT-006 O2 mt D047D047_1PM;2D;2c;3Omy;4U;5AmaCCP mt PM1PM;2D;2c;3Omy;4U;5Ama.pngO2A SUIT-006 - is a coupling control protocol in the N-pathway for isolated mitochondria, tissue homogenate and cells permeabilized before addition to the O2k chamber.
SUIT-007 O2 ce-pce D030D030_ce1;1Dig;1G;2D;2c;3M;4U;5AmaGlutamate anaplerotic pathwayCe1;1Dig;1G;2D;2c;3M;4U;5Ama.pngO2A pce: permeabilized cells - SUIT-007
SUIT-008 O2 ce-pce D025D025_ce1;1Dig;1PM;2D;2c;3G;4S;5U;6Rot;7Ama;8AsTm;9AzdQ-junction ce-pceCe1;1Dig;1PM;2D;2c;3G;4S;5U;6Rot;7Ama;8AsTm;9Azd.pngO2A: for cells-permeabilized cells Lemieux 2017 Sci Rep - SUIT-008
SUIT-008 O2 mt D026D026_1PM;2D;2c;3G;4S;4D;5U;6Rot;7Ama;8AsTm;9AzdQ-junction mtprep1PM;2D;2c;3G;4S;5U;6Rot;7Ama;8AsTm;9Azd.pngO2A: SUIT protocol for mt; mt: isolated mitochondria and tissue homogenate - SUIT-008
SUIT-008 O2 pfi D014D014_1PM;2D;2c;3G;4S;4D;5U;6Rot;7Ama;8AsTm;9Azd1PM;2D;2c;3G;4S;5U;6Rot;7Ama;8AsTm;9Azd.pngO2A - Lemieux 2017 Sci Rep - SUIT-008
SUIT-010 O2 ce-pce D008D008_ce1;ce2Rot;ce3S;ce4D;1Dig;1cDig titration-pceRespirometric test of optimum digitonin concentrationO2A -SUIT-010
SUIT-011 O2 pfi D024D024_1GM;2D;2c;3S;4U;5Rot;6AmaNS physiological maximum capapcity in fibres1GM;2D;2c;3S;4U;5Rot;6Ama.pngO2A pfi: permeabilized fibers - SUIT-011
SUIT-012 O2 ce-pce D052D052_ce1;1Dig;1PM;2D;2c;3G;4U;5AmaN(PGM)Ce1;1Dig;1PM;2D;2c;3G;4U;5Ama.pngO2A Linear coupling control with NADH-linked substrates for pce - SUIT-012
SUIT-012 O2 mt D027D027_1PM;2D;2c;3G;4U;5AmaN CCP mtprep1PM;2D;2c;3G;4U;5Ama.pngO2A Linear coupling control with NADH-linked substrates for isolated mitochondria, tissue homogenate and permeabilized cells- SUIT-012
SUIT-013 AmR ce D023D023_ce1O2 dependence of H2O2 production ce</td>SUIT013 AmR ce D023.png</td>AmR</td>A SUIT-013</td></tr>
SUIT-014 O2 pfi D042</td>D042_1GM;2D;3P;4S;5U;6Rot;7Ama</td>NS(PGM)</td>1GM;2D;2c;3P;4S;5U;6Rot;7Ama.png</td>O2</td>A SUIT-014</td></tr>
SUIT-015 O2 pti D043</td>D043_1OctM;2D;3G;4P;5S;6U;7Rot;8Ama</td>FNS(Oct,PGM)</td>1OctM;2D;3G;4P;5S;6U;7Rot;8Ama.png</td>O2</td>A pti: permeabilized tissue- SUIT-015</td></tr>
SUIT-016 O2 pfi D044</td>D044_1OctM;2D;3G;4S;5Rot;6Omy;7U;8Ama</td>FNS(Oct,GM)</td>1OctM;2D;3G;4S;5Rot;6Omy;7U;7c;8Ama.png</td>O2</td>A pfi: permeabilized fibers- SUIT-016</td></tr>
SUIT-017 O2 mt D046</td>D046_1OctM;2D;3G;3c;4S;5U;6Rot;7Ama</td>FNS(Oct,GM)</td>1OctM;2D;2c;3G;4S;5U;6Rot;7Ama.png</td>O2</td>A: SUIT-017</td></tr>
SUIT-017 O2 pfi D049</td>D049_1OctM;2D;2c;3G;4S;5U;6Rot;7Ama</td>FNS(Oct,GM)</td>1OctM;2D;2c;3G;4S;5U;6Rot;7Ama.png</td>O2</td>A: SUIT-017</td></tr>
SUIT-018 AmR mt D031</td>D031_1GMS;2D;3Ama</td>O2 dependence-H2O2</td>1GMS;2D;3Ama.png</td>AmR</td>A -SUIT-018</td></tr>
SUIT-018 AmR mt D040</td>D040_1GMS;2D;3Ama</td>NS(GM)</td>SUIT-018</td>AmR</td>A: simultaneous determination of O2 and H2O2 flux in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) at high oxygen concentration (tissue hyperoxia)-SUIT-018</td></tr>
SUIT-018 AmR mt D041</td>D041_1GMS;2D;3Ama</td>O2 dependence-H2O2</td>1GMS;2D;3Ama.png</td>AmR</td>A -SUIT-018</td></tr>
SUIT-019 O2 pfi D045</td>D045_1PalM;2D;2c;3Oct;4P;5G;6U;7S;8Rot;9Ama</td>FNS(PalOct,PGM)</td>1PalM;2D;2c;3Oct;4P;5G;6U;7S;8Rot;9Ama.png</td>O2</td>A SUIT-019</td></tr>
SUIT-020 Fluo mt D033</td>D033_1PM;2D;3G;4S;5Rot;6Omy;7U;8Ama</td>NS(PGM)</td>1PM;2D;3G;4S;5Rot;6Omy;7U;8Ama.png</td>Fluo</td>A:short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential in mitochondrial preparations (isolated mitochondria, permeabilized cells, and tissue homogenate)- SUIT-020</td></tr>
SUIT-020 O2 mt D032</td>D032_1PM;2D;2c;3G;4S;5Rot;6Omy;7U;8Ama</td>Q-junction additivity and respiratory control for membrane potential</td>1PM;2D;2c;3G;4S;5Rot;6Omy;7U;8Ama.png</td>O2</td>A: short protocol for determination of O2 flux in mitochondrial preparations (isolated mitochondria, permeabilized cells, and tissue homogenate)-SUIT-020</td></tr>
SUIT-021 Fluo mt D036</td>D036_1GM;2D;3S;4Rot;5Omy;6U;7Ama</td>NS(GM)</td>1GM;2D;3S;4Rot;5Omy;6U;7Ama.png</td>Fluo</td>A: short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential in mitochondrial preparations (isolated mitochondria, permeabilized cells, and tissue homogenate)-SUIT-021</td></tr>
SUIT-021 O2 mt D035</td>D035_1GM;2D;2c;3S;4Rot;5Omy;6U;7Ama</td>NS(GM)</td>1GM;2D;2c;3S;4Rot;5Omy;6U;7Ama.png</td>O2</td>A:short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential in mitochondrial preparations (isolated mitochondria, permabilized cells, and tissue homogenate)-SUIT-021</td></tr>
SUIT-022 O2 ce D051</td>D051_ce1;ce2KCN;ce3SHAM</td>AOX-ce CN+SHAM</td>Ce1;ce2KCN;ce3SHAM.v2.png Ce1;ce2KCN;ce3SHAM.png</td>O2</td>A Determination of the respiration due to the alternative oxidase pathway -SUIT-022</td></tr>
SUIT-023 O2 ce D053</td>D053_ce1;ce2SHAM;ce3KCN</td>AOX-ce SHAM+CN</td>Ce1;ce2SHAM;ce3KCN.png</td>O2</td>A Determination of the respiration due to the cytochrome c oxidase pathway -SUIT-023</td></tr>
SUIT-024 O2 ce-pce D056</td>D056_ce1;1Dig;1PM;2T;2D;3Omy;4Ama</td>N(PM)</td>Ce1;1Dig;1PM;2T;2D;3Omy;4Ama.png</td>O2</td>Determination of the presence of ATPases in mitochondrial preparations - SUIT-024</td></tr>
SUIT-025 O2 mt D057</td>D057_1D;2M;3Oct;3c;4M;5P;6G;7S;8Rot;9Ama</td>FNS(Oct,PGM)</td>1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Rot;9Ama.png</td>O2</td> - SUIT-025</td></tr>
SUIT-026 AmR mt D064</td>D064_1S;2Rot;3D;4Ama</td>RET</td>SUIT-026 AmR mt D064.png</td>AmR</td>A - SUIT-026</td></tr>
SUIT-027 O2 ce-pce D065</td>D065_ce1;1Dig;1M;2D;3M;4P;5G</td>Malate anaplerosis</td>Ce1;1Dig;1M;2D;3M;4P;5G;6Ama.png</td>O2</td>A - SUIT-027</td></tr></table>