Octanoylcarnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine, frequently applied as a substrate for fatty acid oxidation (FAO) in mitochondrial preparations.
MitoPedia topics: Substrate and metabolite
Application in HRR
- Oct: Octanoylcarnitine (DL-Octanoyl-carnitine-HCL, C15H30NO4Cl); TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at RT, desiccate); FW = 323.85.
- Alternative: (±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86.
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
- Preparation of 100 mM stock solution (dissolved in H2O):
- Weigh 50 mg of octanoylcarnitine and dissolve in 1543 µL H2O.
- Divide into 0.2 mL portions.
- Store at -20 °C.
- O2k manual titrations into 2 mL O2k-chamber