SF6847: Difference between revisions
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
=== | {{Chemical_description | ||
:::: '''SF 6847'''/tyrphostin A9/malonoben (C<sub>18</sub>H<sub>22</sub>N<sub> | |abbr=SF6847 | ||
|trivial name=Tyrphostin A9 or Malonoben | |||
|complete name=3,5-bis(1,1-Dimethylethyl)-4-hydroxyphenylmethylenepropanedinitrile | |||
|chem formula=C<sub>18</sub>H<sub>22</sub>N<sub>2</sub>O | |||
|molar mass=282.38 | |||
|vendor=Sigma-Aldrich | |||
|product number=T182 | |||
|store at=RT | |||
|sensitivity= | |||
|cas=10537-47-0 | |||
|h statements=H301, H311 | |||
|h info=toxic if swallowed, toxic in contact with skin | |||
}}<!--:::: '''SF 6847'''/tyrphostin A9/malonoben (C<sub>18</sub>H<sub>22</sub>N<sub>2</sub>O, [[3,5-bis(1,1-Dimethylethyl)-4-hydroxyphenyl]methylene]propanedinitrile; Sigma T182, 5 mg: M = 282.38 g·mol<sup>-1</sup>. Alternative source: Enzo, BML-EI215. | |||
:::: <span style="color:#8B008B"> '''Caution:''' Toxic!</span>--> | |||
::: '''Preparation of | ::: '''Preparation of 25 mM stock solution''' (dissolved in ethanol abs.): | ||
::::# | ::::# Weigh 5 mg in a dark glass vial, dissolve in 708.3 µL ethanol. | ||
::::# Store in glass vials at -20 °C. | ::::# Store in dark glass vials at -20 °C. | ||
::: '''Preparation of 50 µM stock solution''' (dissolved in | ::: '''Preparation of 50 µM stock solution''' (dissolved in ethanol abs.) for '''2-mL O2k-chamber''': | ||
::::# | ::::# Mix 998 µL ethanol abs. and 2 µL of the 25 mM stock solution. | ||
::::# Divide into 0.1 ml aliquotes, | ::::# Divide into 0.1 ml aliquotes, use dark glass vials. | ||
::::# Store at -20 °C. | ::::# Store at -20 °C. | ||
::: '''O2k manual titrations''' | ::: » '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
::::* Titration volume: 1 | ::::* Titration volume ('''2-mL O2k-chamber'''): 1 µL steps of 50 µM stock solution using a 10 µL Hamilton syringe. | ||
::::* Final concentration: 25 nM steps. | ::::* Final concentration: 25 nM steps. | ||
::: '''Preparation of 12.5 µM stock solution''' (dissolved in ethanol abs.) for '''0.5-mL O2k-chamber''': | |||
::::# Add 300 µL ethanol abs. and 100 µL of the 50 µM stock solution. | |||
::::# Divide into 0.1 ml aliquots in dark glass vials. Store at -20 °C. | |||
::: » '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | |||
::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL of 12.5 µM stock solution using a 10 µL Hamilton syringe. | |||
::::* Final concentration: 25 nM steps. | |||
:::: '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening. | :::: '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening. |
Revision as of 11:57, 22 June 2021
Description
SF6847 (C18H22N2O), also known as tyrphostin A9 or malonoben, is a protonophore and a very potent uncoupler of oxidative phosphorylation, being used in the nM range. Like all uncouplers, SF6847 concentrations must be titrated carefully to evaluate the optimum concentration for maximum stimulation of mitochondrial respiration, particularly to avoid inhibition of respiration at higher concentrations.
Abbreviation: SF6847
Reference: Chinopoulos 2014 Methods Enzymol, Terada 1984 Biochim Biophys Acta
Application in HRR
- SF6847: Tyrphostin A9 or Malonoben (3,5-bis(1,1-Dimethylethyl)-4-hydroxyphenylmethylenepropanedinitrile; C18H22N2O), Sigma-Aldrich: T182, store at RT, CAS: 10537-47-0, M = 282.38 g·mol-1
- Hazard statements: H301, H311; toxic if swallowed, toxic in contact with skin
- SF6847: Tyrphostin A9 or Malonoben (3,5-bis(1,1-Dimethylethyl)-4-hydroxyphenylmethylenepropanedinitrile; C18H22N2O), Sigma-Aldrich: T182, store at RT, CAS: 10537-47-0, M = 282.38 g·mol-1
- Preparation of 25 mM stock solution (dissolved in ethanol abs.):
- Weigh 5 mg in a dark glass vial, dissolve in 708.3 µL ethanol.
- Store in dark glass vials at -20 °C.
- Preparation of 25 mM stock solution (dissolved in ethanol abs.):
- Preparation of 50 µM stock solution (dissolved in ethanol abs.) for 2-mL O2k-chamber:
- Mix 998 µL ethanol abs. and 2 µL of the 25 mM stock solution.
- Divide into 0.1 ml aliquotes, use dark glass vials.
- Store at -20 °C.
- Preparation of 50 µM stock solution (dissolved in ethanol abs.) for 2-mL O2k-chamber:
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 1 µL steps of 50 µM stock solution using a 10 µL Hamilton syringe.
- Final concentration: 25 nM steps.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Preparation of 12.5 µM stock solution (dissolved in ethanol abs.) for 0.5-mL O2k-chamber:
- Add 300 µL ethanol abs. and 100 µL of the 50 µM stock solution.
- Divide into 0.1 ml aliquots in dark glass vials. Store at -20 °C.
- Preparation of 12.5 µM stock solution (dissolved in ethanol abs.) for 0.5-mL O2k-chamber:
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (0.5-mL O2k-chamber): 1 µL of 12.5 µM stock solution using a 10 µL Hamilton syringe.
- Final concentration: 25 nM steps.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
- Bioblast links: Uncoupling - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
- » Artefacts by single dose uncoupling
- » ATP synthase
- » CCCP
- » Coupling-control protocol
- » DNP
- » Dyscoupled respiration
- » FCCP
- » Is respiration uncoupled - noncoupled - dyscoupled?
- » Noncoupled respiration: Discussion
- » Uncoupler
- » Uncoupled respiration - see » Noncoupled respiration
- » Uncoupling proteins
- » Uncoupling protein 1
- » Uncoupler titrations - Optimum uncoupler concentration
- Specific
- Respiratory states and control ratios
- » Biochemical coupling efficiency
- » Coupling-control state
- » Electron-transfer-pathway state
- » Electron-transfer pathway
- ET capacity
- » E-L coupling efficiency
- » Flux control efficiency
- » Flux control ratio
- » LEAK-control ratio
- » LEAK respiration
- » Noncoupled respiration
- » OXPHOS
- » OXPHOS capacity; » State 3
- » OXPHOS-control ratio, P/E ratio
- » Respiratory acceptor control ratio
- » ROUTINE-control ratio
- » ROUTINE respiration
- » ROUTINE state
- » State 3u
- » State 4
- » Uncoupling-control ratio UCR
- Respiratory states and control ratios
- Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1
- Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
- General (alphabetical order)
- Other keyword lists
MitoPedia topics:
Uncoupler