O2k-PublicationAlert2020 Archive: Difference between revisions

Revision as of 11:31, 4 May 2020

Currently Answered Questions from the »Oroboros Ecosystem Agenda«

May 2020

May-12 Tu

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Mitochondria - a driving force in adipocyte differentiation

- from the O2k-Network »CZ Pilsen Kuncova J«
Kladnická I, Čedíková M, Kripnerová M, Dvořáková J, Kohoutová M, Tůma Z, Müllerová D, Kuncová J (2019) Mitochondrial respiration of adipocytes differentiating from human mesenchymal stem cells derived from adipose tissue. Physiol Res 68:287-96. »Bioblast link«

Apr 2020

Apr-24 Fr

ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the electron transfer system.

- »Hatefi 1962 J Biol Chem-XLII«

Apr-21 Tu

Citrate synthase activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.

- »MiPNet17.04 CitrateSynthase«

Apr-13 Mo

Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?

DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the mitochondrial preparation
- »SUIT-024«

Apr-08 We

The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.

DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum digitonin concentration.
- »SUIT-010«

Apr-02 Th

Do you want to search the best SUIT protocol for your research questions?

- Use the SUITbrowser: »SUITbrowser«

Mar 2020

Mar-16 Mo

The standardized coupling control protocol to explore the bioenergetics of living cells

DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions.
- »SUIT-003«

Mar-11 We

Does blebbistatin reduce oxygen dependence of respiration of permeabilized muscle fibers? This is one of the questions addressed in the COST Action CA15203 MitoEAGLE WG2.

- »Blebbistatin«

Mar-06 Fr

Explore metabolic profiles and specific modulations of mitochondrial pathways in health and disease with SUIT reference protocols.

- »RP1« and »RP2«

Mar-03 Tu

Rapamycin - more differences or more in common with other mitObesity drugs?

- »Rapamycin«

Feb 2020

Feb-26 We

It is essential to titrate uncouplers in multiple steps for respirometric measurement of electron transfer capacity.

- »Noncoupled respiration«

Feb-24 Mo

More research is needed on the impact of viral infection on mitochondrial function.

- »Viruses and mitochondrial medicine«

Feb-19 We

Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function.

- »Spermidine«

Feb-18 Tu

Why do we fight gas bubbles?

- Dive deep into »Oxygen solubility«

Feb-12 We

What do metformin, melatonin and other bioactive mitObesity compounds have in common?

- »MitObesity drugs«

Jan 2020

Jan-16 Th

Oxygen flux

- which SI units should be used?

Did you ever think that units for body mass could be an issue?

- »Body mass«

Jan-08 We

Force and Energy: this we all need in the New Year - and MitoPedia may help to clarify the difference.

@@ Line 3: / Line 3: @@
 == May 2020 ==
-* '''May-07 Th'''
+* '''May-12 Tu'''
 :{|
-| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
+| width="100" align="center" [[File:O2k-Publications.jpg|80px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|Do you know how to evaluate '''[[Fatty acid oxidation pathway control state|fatty acid oxidation (FAO, F-pathway)''']] avoiding to overestimate it?'''
+|''' Mitochondria - a driving force in adipocyte differentiation''' <br>
-Did you know that '''malate alone might support mt-respiration''' in [[Mitochondrial preparations| mitochondrial preparations]], if '''malate anaplerotic pathways''' ([[Malic enzyme|e.g. '''mt-malic enzyme''']]) are present? <br>
+- from the O2k-Network »[[CZ Pilsen Kuncova J]]«<br>
-- Learn more about - »'''[[Malate anaplerotic pathway control state|malate anaplerotic pathway control state]]'''«<br>
+Kladnická I, Čedíková M, Kripnerová M, Dvořáková J, Kohoutová M, Tůma Z, Müllerová D, Kuncová J (2019) Mitochondrial respiration of adipocytes differentiating from human mesenchymal stem cells derived from adipose tissue. Physiol Res 68:287-96.  [[Kladnicka 2019 Physiol Res|»Bioblast link«]]
-- '''DatLab 7.4''' provides a step-by-step guide through the '''SUIT-027''' to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''«<br>
-- Since [[Fatty acid oxidation pathway control state|F-pathway]] can be overestimated due to anaplerotic reactions, '''SUIT-002''' was specially designed to give information on '''F-pathway in OXPHOS state avoiding FAO overestimation''' in the presence of [[Anaplerosis|anaplerotic pathways]] - »'''[[SUIT-002 |SUIT-002]]'''«
-|}
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