MitoPediaAlert2020 Archive: Difference between revisions
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- DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum [[digitonin]] concentration. - ยป'''[[SUIT-010 |SUIT-010]]'''ยซ | - DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum [[digitonin]] concentration. - ยป'''[[SUIT-010 |SUIT-010]]'''ยซ | ||
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::::[[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]'''Do you want to search the best [[SUIT]] protocol for your research questions?'''ย Use the SUITbrowser: ยป'''[[SUITbrowser]]'''ยซ | |||
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Revision as of 12:33, 30 April 2020
- Currently Answered Questions from the ยปOroboros Ecosystem Agendaยซ
May 2020
- May-07 Th
- Do you know how to evaluate fatty acid oxidation (FAO, F-pathway) avoiding to overestimate it?
- Did you know that malate alone might support mt-respiration in mitochondrial preparations, if malate anaplerotic pathways (e.g. mt-malic enzyme) are present?
- - Learn more about - ยปmalate anaplerotic pathway control stateยซ
- - DatLab 7.4 provides a step-by-step guide through the SUIT-027 to evaluate the malate anaplerotic pathway - ยปSUIT-027ยซ
- - Since F-pathway can be overestimated due to anaplerotic reactions, SUIT-002 was specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways - ยปSUIT-002ยซ
Apr 2020
- Apr-24 Fr
ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the electron transfer system.
- Apr-21 Tu
Citrate synthase activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.
- Apr-13 Mo
Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases? - DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the mitochondrial preparation - ยปSUIT-024ยซ
- Apr-08 We
The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage. - DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum digitonin concentration. - ยปSUIT-010ยซ
- Apr-02 Th
- Do you want to search the best SUIT protocol for your research questions? Use the SUITbrowser: ยปSUITbrowserยซ
Mar 2020
- Mar-16 Mo
The standardized coupling control protocol to explore the bioenergetics of living cells - DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. - ยปSUIT-003ยซ
- Mar-11 We
Does blebbistatin reduce oxygen dependence of respiration of permeabilized muscle fibers? This is one of the questions addressed in the COST Action CA15203 MitoEAGLE WG2. - ยปBlebbistatinยซ
- Mar-06 Fr
Explore metabolic profiles and specific modulations of mitochondrial pathways in health and disease with SUIT reference protocols.
- Mar-03 Tu
Rapamycin - more differences or more in common with other mitObesity drugs? - ยปRapamycinยซ
Feb 2020
- Feb-26 We
It is essential to titrate uncouplers in multiple steps for respirometric measurement of electron transfer capacity. - ยปNoncoupled respirationยซ
- Feb-24 Mo
- Feb-19 We
Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function. - ยปSpermidineยซ
- Feb-18 Tu
Why do we fight gas bubbles? - Dive deep into ยปOxygen solubilityยซ
- Feb-12 We
What do metformin, melatonin and other bioactive mitObesity compounds have in common? - ยปMitObesity drugsยซ
Jan 2020
- Jan-16 Th
Oxygen flux - which SI units should be used?
Did you ever think that units for body mass could be an issue? - ยปBody massยซ
- Jan-08 We
Force and Energy: this we all need in the New Year - and MitoPedia may help to clarify the difference.