MitoEAGLE preprint 2017-09-21

Gnaiger E, Ahn B, Alves MG, Beard DA, Ben-Shachar D, Bishop D, Breton S, Brown GC, Brown RA, Buettner GR, Bumsoo A, Cervinkova Z, Chicco AJ, Coen PM, Collins JL, Crisóstomo L, Davis MS, Dias T, Doerrier C, Fell DA, Filipovska A, Garcia-Roves PM, Garcia-Souza LF, Gonzalo H, Goodpaster BH, Han J, Harrison DK, Hellgren KT, Hernansanz P, Hoppel CL, Iglesias-Gonzalez J, Irving BA, Iyer S, Jansen-Dürr P, Käämbre T, Kane DA, Komlodi T, Kuang J, Laner V, Lee HK, Lemieux H, Lucchinetti E, Makrecka-Kuka M, Meszaros AT, Moisoi N, Molina AJA, Montaigne D, Moore AL, Murray AJ, Porter RK, Nozickova K, Oliveira PF, Oliveira PJ, Orynbayeva Z, Palmeira CM, Pesta D, Petit PX, Pichaud N, Pirkmajer S, Prochownik EV, Renner-Sattler K, Rohlena J, Rossiter HB, Salvadego D, Scatena R, Schartner M, Scheibye-Knudsen M, Scott GR, Singer D, Sobotka O, Spinazzi M, Stocker R, Sumbalova Z, Tanaka M, Tandler B, Tepp K, Tronstad KJ, Tyrrell DJ, Velika B, Vendelin M, Vercesi AE, Watala C, Wei YH, Wieckowski MR, Wolff J, Wüst RCI, Zaugg M, Zorzano A (2017) The protonmotive force and respiratory control: Building blocks of mitochondrial physiology Part 1. MitoEAGLE Network COST Action CA15203.

» MitoEAGLE preprint Open Access

Gnaiger E, Ahn B, Alves MG, Beard DA, Ben-Shachar D, Bishop D, Breton S, Brown GC, Brown RA, Buettner GR, Bumsoo A, Cervinkova Z, Chicco AJ, Coen PM, Collins JL, Crisostomo L, Davis MS, Dias T, Doerrier C, Fell DA, Filipovska A, Garcia-Roves PM, Garcia-Souza LF, Gonzalo H, Goodpaster BH, Han J, Harrison DK, Hellgren KT, Hernansanz P, Hoppel CL, Iglesias-Gonzalez J, Irving BA, Iyer S, Jansen-Duerr P, Kaeaembre T, Kane DA, Komlodi T, Kuang J, Laner V, Lee HK, Lemieux H, Lucchinetti E, Makrecka-Kuka M, Meszaros AT, Moisoi N, Molina AJA, Montaigne D, Moore AL, Murray AJ, Porter RK, Nozickova K, Oliveira PF, Oliveira PJ, Orynbayeva Z, Palmeira CM, Pesta D, Petit PX, Pichaud N, Pirkmajer S, Prochownik EV, Renner-Sattler K, Rohlena J, Rossiter HB, Salvadego D, Scatena R, Schartner M, Scheibye-Knudsen M, Scott GR, Singer D, Sobotka O, Spinazzi M, Stocker R, Sumbalova Z, Tanaka M, Tandler B, Tepp K, Tronstad KJ, Tyrrell DJ, Velika B, Vendelin M, Vercesi AE, Watala C, Wei YH, Wieckowski MR, Wolff J, Wuest RCI, Zaugg M, Zorzano A (2017) MitoEAGLE Network

Abstract: Clarity of concepts and consistency of nomenclature are trademarks of a research field across its specializations, facilitating transdisciplinary communication and education. As research and knowledge of mitochondrial physiology expand, the necessity for harmonizing nomenclature concerning mitochondrial respiratory states and rates has become apparent. Peter Mitchell’s concept of the protonmotive force establishes the link between the electrical and chemical components of energy transformation and its coupling in oxidative phosphorylation. This unifying concept provides the framework for developing a consistent terminology of mitochondrial physiology and bioenergetics. We follow IUPAC guidelines on general terms of physical chemistry, extended by concepts of open systems and irreversible thermodynamics. We align the nomenclature of classical bioenergetics on respiratory states with a concept-driven constructive terminology to address the meaning of each respiratory state. Standards for evaluation of respiratory coupling states must be followed for the development of databases of mitochondrial respiratory function in species, tissues and cells studied under diverse physiological and experimental conditions. • Keywords: Mitochondrial respiratory control, coupling control, mitochondrial preparations, protonmotive force, chemiosmotic theory, oxidative phosphorylation, OXPHOS, efficiency, electron transfer system, ETS; proton leak, LEAK, residual oxygen consumption, ROX, State 2, State 3, State 4, normalization, flow, flux • Bioblast editor: Gnaiger E

Labels: MiParea: Respiration, mt-Awareness 

Preparation: Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria  Enzyme: Marker enzyme  Regulation: Coupling efficiency;uncoupling, Flux control, mt-Membrane potential, Uncoupler  Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.