User:Spitzer Gudrun

Chemical preparation

• Liste mit Substrates, Inhibitors, Uncouplers, ect.: MiPNet09.12_O2k-Titrations

• Chemical preparation list

Added sV Instructions to following pages

Same concentration for standard and small volume chamber

• Malate
• Glutamate
• Succinate
• Octanoylcarnitine
• ADP
• ATP
• Glucose: check Luis for the second paragraph that is not in titration list
• Glycerophosphate

Different concentration for different chambers

Concentrations prepared separately

• Pyruvate
• Ascorbate
• TMPD
• Cytochrome_c
• KCN ask Luiz or Marco for feasability (how much stock solution is needed, volume of aliquots...)

Conc for sV-chamber by dilution

• CCCP
• Rotenone
• Malonate
• Antimycin A
• Myxothiazol
• Sodium azide

ToDo

• Oligomycin who works with Omy? There are two concentrations in the wiki, only the lower conc is on the titrations-pdf. Write instructions for both concentrations (and both chambers)? The second concentration is achieved by dilution - which dilution would be appropriate for the sV-concentration? Luiz: give instructions for the low conc only. The high conc is not recommended any more
• Carboxyatractyloside 2 conc indicated, only one is in the O2k-titration table. Luiza sagt, die höhere conc ist überholt und kann eigentlich gelöscht werden.
• Salicylhydroxamic acid alle sage, Maria ist die Expertin und die hat das noch nie für's sV-chamber benützt
• Digitonin
• Catalase in MiR06
• Hydrogen peroxide

Wiki editing

Wikipedia Cheatsheet

one conc	diff conc
ROW1	ROW1/COL2
ROW2	ROW2/COL2