User:Spitzer Gudrun
From Bioblast
Chemical preparation
- Liste mit Substrates, Inhibitors, Uncouplers, ect.: MiPNet09.12_O2k-Titrations
Added sV Instructions to following pages
Same concentration for standard and small volume chamber
- Malate
- Glutamate
- Succinate
- Octanoylcarnitine
- ADP
- ATP
- Glucose: check Luis for the second paragraph that is not in titration list
- Glycerophosphate
Different concentration for different chambers
Concentrations prepared separately
- Pyruvate
- Ascorbate
- TMPD
- Cytochrome_c
- KCN aks Luiz or Marco for feasability (same vol as for 2mL-chamber? Even lower vol, because lower titration volumes for sV-chamber?
Conc for sV-chamber by dilution
ToDo
- Oligomycin who works with Omy? There are two concentrations in the wiki, only the lower conc is on the titrations-pdf. Write instructions for both concentrations (and both chambers)? The second concentration is achieved by dilution - which dilution would be appropriate for the sV-concentration?
- Carboxyatractyloside 2 conc indicated, only one is in the O2k-titration table
Wiki editing
one conc | diff conc |
---|---|
ROW1 | ROW1/COL2 |
ROW2 | ROW2/COL2 |