User:Spitzer Gudrun: Difference between revisions
From Bioblast
No edit summary |
|||
| Line 23: | Line 23: | ||
* [[TMPD]] | * [[TMPD]] | ||
* [[Cytochrome_c]] | * [[Cytochrome_c]] | ||
* [[KCN]] | * [[KCN]] ask Luiz or Marco for feasability (how much stock solution is needed, volume of aliquots...) | ||
==== Conc for sV-chamber by dilution ==== | ==== Conc for sV-chamber by dilution ==== | ||
* [[CCCP]] | * [[CCCP]] | ||
Revision as of 11:13, 2 November 2020
Chemical preparation
- Liste mit Substrates, Inhibitors, Uncouplers, ect.: MiPNet09.12_O2k-Titrations
Added sV Instructions to following pages
Same concentration for standard and small volume chamber
- Malate
- Glutamate
- Succinate
- Octanoylcarnitine
- ADP
- ATP
- Glucose: check Luis for the second paragraph that is not in titration list
- Glycerophosphate
Different concentration for different chambers
Concentrations prepared separately
- Pyruvate
- Ascorbate
- TMPD
- Cytochrome_c
- KCN ask Luiz or Marco for feasability (how much stock solution is needed, volume of aliquots...)
Conc for sV-chamber by dilution
ToDo
- Oligomycin who works with Omy? There are two concentrations in the wiki, only the lower conc is on the titrations-pdf. Write instructions for both concentrations (and both chambers)? The second concentration is achieved by dilution - which dilution would be appropriate for the sV-concentration? Luiz: give instructions for the low conc only. The high conc is not recommended any more
- Carboxyatractyloside 2 conc indicated, only one is in the O2k-titration table. Luiza sagt, die höhere conc ist überholt und kann eigentlich gelöscht werden.
- Salicylhydroxamic acid alle sage, Maria ist die Expertin und die hat das noch nie für's sV-chamber benützt
- Digitonin
- Catalase in MiR06
- Hydrogen peroxide
Wiki editing
| one conc | diff conc |
|---|---|
| ROW1 | ROW1/COL2 |
| ROW2 | ROW2/COL2 |
