User:Spitzer Gudrun: Difference between revisions
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* [[Oligomycin]] who works with Omy? There are two concentrations in the wiki, only the lower conc is on the titrations-pdf. Write instructions for both concentrations (and both chambers)? The second concentration is achieved by dilution - which dilution would be appropriate for the sV-concentration? | * [[Oligomycin]] who works with Omy? There are two concentrations in the wiki, only the lower conc is on the titrations-pdf. Write instructions for both concentrations (and both chambers)? The second concentration is achieved by dilution - which dilution would be appropriate for the sV-concentration? Luiz: give instructions for the low conc only. The high conc is not recommended any more | ||
* [[Carboxyatractyloside]] 2 conc indicated, only one is in the O2k-titration table | * [[Carboxyatractyloside]] 2 conc indicated, only one is in the O2k-titration table | ||
* [[Salicylhydroxamic acid]] | * [[Salicylhydroxamic acid]] | ||
* [[Digitonin]] | * [[Digitonin]] | ||
* [[Catalase]] in MiR06 | * [[Catalase]] in MiR06 | ||
* [[Hydrogen peroxide]] | * [[Hydrogen peroxide]] | ||
== Wiki editing == | == Wiki editing == | ||
Revision as of 13:02, 29 October 2020
Chemical preparation
- Liste mit Substrates, Inhibitors, Uncouplers, ect.: MiPNet09.12_O2k-Titrations
Added sV Instructions to following pages
Same concentration for standard and small volume chamber
- Malate
- Glutamate
- Succinate
- Octanoylcarnitine
- ADP
- ATP
- Glucose: check Luis for the second paragraph that is not in titration list
- Glycerophosphate
Different concentration for different chambers
Concentrations prepared separately
- Pyruvate
- Ascorbate
- TMPD
- Cytochrome_c
- KCN aks Luiz or Marco for feasability (same vol as for 2mL-chamber? Even lower vol, because lower titration volumes for sV-chamber?
Conc for sV-chamber by dilution
ToDo
- Oligomycin who works with Omy? There are two concentrations in the wiki, only the lower conc is on the titrations-pdf. Write instructions for both concentrations (and both chambers)? The second concentration is achieved by dilution - which dilution would be appropriate for the sV-concentration? Luiz: give instructions for the low conc only. The high conc is not recommended any more
- Carboxyatractyloside 2 conc indicated, only one is in the O2k-titration table
- Salicylhydroxamic acid
- Digitonin
- Catalase in MiR06
- Hydrogen peroxide
Wiki editing
| one conc | diff conc |
|---|---|
| ROW1 | ROW1/COL2 |
| ROW2 | ROW2/COL2 |
