User:Spitzer Gudrun: Difference between revisions
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=== Different concentration for different chambers === | === Different concentration for different chambers === | ||
==== Concentrations prepared separately ==== | |||
::::* [[Pyruvate]] | ::::* [[Pyruvate]] | ||
::::* [[Ascorbate]] | ::::* [[Ascorbate]] | ||
| Line 25: | Line 27: | ||
::::* [[Rotenone]] | ::::* [[Rotenone]] | ||
::::* [[Malonate]]: ask Luiz: does it make sense to half the amount of malonate and double the H2O to get the conc for the sV-chamber? A lower amount of malonate will probably cause problems on the scale and with pH titration. Or is malonate cheap enough to take the same amount in a larger volume | ::::* [[Malonate]]: ask Luiz: does it make sense to half the amount of malonate and double the H2O to get the conc for the sV-chamber? A lower amount of malonate will probably cause problems on the scale and with pH titration. Or is malonate cheap enough to take the same amount in a larger volume | ||
==== Conc for sV-chamber by dilution ==== | |||
::::* [[Antimycin A]] | |||
== ToDo == | == ToDo == | ||
::::* [[Myxothiazol]] | ::::* [[Myxothiazol]] | ||
::::* [[Sodium azide ]] | ::::* [[Sodium azide ]] | ||
Revision as of 13:04, 27 October 2020
Chemical preparation
- Liste mit Substrates, Inhibitors, Uncouplers, ect.: MiPNet09.12_O2k-Titrations
Added sV Instructions to following pages
Same concentration for standard and small volume chamber
- Malate
- Glutamate
- Succinate
- Octanoylcarnitine
- ADP
- ATP
- Glucose: check Luis for the second paragraph that is not in titration list
- Glycerophosphate
Different concentration for different chambers
Concentrations prepared separately
- Pyruvate
- Ascorbate
- TMPD
- Cytochrome_c
- CCCP
- Rotenone
- Malonate: ask Luiz: does it make sense to half the amount of malonate and double the H2O to get the conc for the sV-chamber? A lower amount of malonate will probably cause problems on the scale and with pH titration. Or is malonate cheap enough to take the same amount in a larger volume
Conc for sV-chamber by dilution
ToDo
Wiki editing
| one conc | diff conc |
|---|---|
| ROW1 | ROW1/COL2 |
| ROW2 | ROW2/COL2 |
