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| |- | | |- |
| | 5Ama | | | 5* |
| | [[ROX]] | | | [[ROX]] |
| | ย | | | ย |
| |ย ย | | |ย ย |
| | 1X;2D;2c;3(Omy);4U;5Ama | | | 1X;2D;2c;3(Omy);4U;5* |
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| *{{Template:SUIT Ama}} | | *{{Template:SUIT ROX}} |
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| |} | | |} |
Revision as of 11:42, 6 March 2019
Steps and respiratory states
File:CCP for mt preparations.png
Step
|
State
|
Pathway
|
Q-junction
|
Comment - Events (E) and Marks (M)
|
1X
|
XL(n)
|
|
|
1X
- Electron-transfer-pathway state will depend on the substrate/inhibitor combinations added to fuel the mitochondrial respiration
- Non-phosphorylating resting state (LEAK state); LEAK respiration L(n) in the absence of ADP, ATP, AMP (no adenylates).
|
2D
|
XP
|
|
|
1X;2D
|
2c
|
XcP
|
|
|
1X;2D;2c
- Electron-transfer-pathway state will depend on the substrate/inhibitor combinations added to fuel the mitochondrial respiration
- OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
- Addition of cytochrome c yields a test for integrity of the mtOM (cytochrome c control efficiency). Stimulation by added cytochrome c would indicate an injury of the mtOM and limitation of respiration in the preceding state without added c due to loss of cytochrome c. Typically, cytochrome c is added immediately after the earliest ADP-activation step (OXPHOS capacity P with saturating [ADP]).
|
3(Omy)
|
XL(Omy)
|
|
|
1X;2D;2c;3(Omy)
- Electron-transfer-pathway state will depend on the substrate/inhibitor combinations added to fuel the mitochondrial respiration
- Non-phosphorylating resting state (LEAK state); LEAK-respiration, L(Omy), after blocking the ATP synthase with oligomycin.
- The addition of oligomycin is optional depending on the experimental question to resolve. If it is known that Ln has the same values for LOmy for a kind of sample and substrates, this step may be skipped. If the carrier of oligomycin (EtOH) is used instead, it is possible to evaluate whether it affects respiration. In these cases, LEAK state is not reached again.
|
4U
|
XE
|
|
|
1X;2D;2c;3(Omy);4U
|
5*
|
ROX
|
|
|
1X;2D;2c;3(Omy);4U;5*
- Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated either after inhibition of CIII (e.g. antimycin A, myxothiazol), CIV (e.g. Cyanide) or in the absence of endogenous fuel-substrates. Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration.
|
Step
|
Respiratory state
|
Pathway control
|
ET-Complex
|
Comment
|
## AsTm
|
AsTmE
|
CIV
|
CIV
|
|
## Azd
|
CHB
|
|
|
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- ยป Coupling control state
- ยป ET capacity
- ยป OXPHOS capacity
- ยป LEAK respiration
- Pathway control
- ยป Electron transfer pathway
- ยป Fatty acid oxidation pathway control state, F
- ยป NADH electron transfer-pathway state, N
- ยป Succinate pathway control state, S
- ยป NS-pathway control state, NS
- ยป Glycerophosphate pathway control state, Gp
- ยป Complex IV single step, CIV
- ยป Anaplerotic pathway control state
- Main fuel substrates
- ยป Glutamate, G
- ยป Glycerophosphate, Gp
- ยป Malate, M
- ยป Octanoylcarnitine, Oct
- ยป Pyruvate, P
- ยป Succinate, S
- Glossary
- ยป List of SUIT states
- ยป SUIT concept
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