Template:SUIT-004: Difference between revisions
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| 6Ama | | 6Ama | ||
| [[ROX]] | | [[ROX]] | ||
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| 1PM;2D;3U;4S;5Rot;6Ama | | 1PM;2D;3U;4S;5Rot;6Ama |
Revision as of 12:08, 14 January 2019
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) | |
---|---|---|---|---|---|
1PM | PML | N | CI | PML or PM_L: Pyruvate & malate, N-LEAK respiration, NL
NADH-linked substrates (type N-pathway to Q). Template:SUIT L n | |
2D | PMP | N | CI | 1PM;2D
NADH-linked substrates (type N-pathway to Q). OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. | |
2c | PMcP | N | CI | 1PM;2D;2c
NADH-linked substrates (type N-pathway to Q). OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. Addition of cytochrome c yields a test for integrity of the mtOM (cytochrome c control efficiency). Stimulation by added cytochrome c would indicate an injury of the mtOM and limitation of respiration in the preceding state without added c due to loss of cytochrome c. Typically, cytochrome c is added immediately after the earliest ADP-activation step (OXPHOS capacity P with saturating [ADP]). | |
3U | PME | NS | CI&II | 1PM;2D;3U
Respiratory stimulation by simultaneous action of type N substrates & succinate, with convergent electron flow in the NS-pathway for reconstitution of TCA cycle function. Uncoupler titration (avoiding inhibition by high uncoupler concentrations) to obtain electron transfer (ET) capacity E (noncoupled ET-state). Test for limitation of OXPHOS capacity P by the phosphorylation system (ANT, ATP synthase, phosphate transporter) relative to ET capacity E in mt-preparations: E-P control efficiency and E-L coupling efficiency. In living cells: E-R control efficiency and E-L coupling efficiency. | |
4S | PMSP | NS | CI&II | 1PM;2D;3U;4S
NADH-linked substrates (type N-pathway to Q). & Succinate, S ( type S-pathway to Q). Respiratory stimulation by simultaneous action of type N substrates & succinate, with convergent electron flow in the NS-pathway for reconstitution of TCA cycle function. OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
| |
5Rot | SE | S | CII | 1PM;2D;3U;4S;5Rot
Succinate pathway control state (S-pathway) after inhibiting CI with rotenone, which also inhibits the F-pathway. Noncoupled electron transfer state, ET state, with ET capacity E. | |
6Ama | ROX | 1PM;2D;3U;4S;5Rot;6Ama | Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt). |
Step | Respiratory state | Pathway control | ET-Complex | Comment |
---|---|---|---|---|
## AsTm | AsTmE | CIV | CIV | |
## Azd | CHB |