Difference between revisions of "Template:MitoPediaAlert2021"
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:::::: <big>''Currently Answered Questions from the »[[OROBOROS_INSTRUMENTS|Oroboros Ecosystem Agenda]]« | :::::: <big>''Currently Answered Questions from the »[[OROBOROS_INSTRUMENTS|Oroboros Ecosystem Agenda]]« | ||
</big>__TOC__ | </big>__TOC__ | ||
== Nov 2021 == | |||
*'''Nov-24 We''' | |||
:{| | |||
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]] | |||
|'''Mitochondrial H<sub>2</sub>O<sub>2</sub> production was discovered 50 years ago by the group of Britton Chance'''<br> | |||
Check out how to measure H<sub>2</sub>O<sub>2</sub> production using the Amplex UltraRed assay in combination with high-resolution respirometry.'''<br> | |||
- »[[Loschen 1971 FEBS Letters]]« and »[[Amplex UltraRed]]« and »[[O2k-Fluo LED2-Module]]« »[[O2k-Fluo Smart-Module]]« <br> | |||
- communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]« <br> | |||
|} | |||
== Jul 2021 == | == Jul 2021 == |
Revision as of 10:24, 26 November 2021
- Currently Answered Questions from the »Oroboros Ecosystem Agenda«
Nov 2021
- Nov-24 We
Mitochondrial H2O2 production was discovered 50 years ago by the group of Britton Chance
Check out how to measure H2O2 production using the Amplex UltraRed assay in combination with high-resolution respirometry.
- »Loschen 1971 FEBS Letters« and »Amplex UltraRed« and »O2k-Fluo LED2-Module« »O2k-Fluo Smart-Module«
- communicated by »Komlodi Timea« and »Schmitt Sabine«
Jul 2021
- Jul-07 We
TPP+ for potentiometric detection of mt-membrane potential (mtMP) or fluorometry with safranin?
Safranin enables measurement of the relationship between fluorescence intensity and mtMP, and if linear, the absolute mtMP can be calculated. TPP+ enables calibration for measurement of mtMP.
- »TPP+ vs safranin«
Jun 2021
- Jun-24 Th
Do you know how to select the correct fluorescence sensors and filter set?
Using different fluorescence sensors, LED and photodiode filters, the O2k-Fluo Smart-Module (or O2k-Fluo LED2-Module) enables measurement of hydrogen peroxide, ATP, mitochondrial membrane potential, calcium, and numerous other applications open for O2k-user innovation.
- »Selecting the Smart Fluo-Sensor« and »Selecting a Filter Set«
May 2021
- May-24 Mo
Immediately after switching off the light, a sharp peak of light-enhanced dark respiration is observed in algae.
- May-11 Tu
Measuring succinate pathway (S-pathway) in living cells
Using MitoKit-CII – cell permeable succinate and malonate, the S-pathway can be measured
- »SUIT-003 O2 ce D060«
- communicated by »Cecatto Cristiane« and »Komlodi Timea«
Apr 2021
Mar 2021
- Mar-16 Tu
CI inhibitors like rotenone inhibit fatty acid oxidation (FAO)-linked respiration
Inhibition of CI leads to accumulation of NADH in the mt-matrix. The resultant lack of NAD+ blocks FAO.
- »Fatty acid oxidation pathway control state«, »Mitochondrial Pathways: Chapter 5«
- communicated by »Cardoso Luiza« and »Cecatto Cristiane«
- Mar-11 Th
Internal normalization of rate – what is the difference between flux control ratio and flux control efficiency?
A flux control ratio is the ratio of oxygen flux in a specific respiratory control state, normalized for maximum flux in a common reference state. Flux control efficiency expresses the control of respiration as a fractional change of oxygen flux or flow between protocol steps. Both values vary between theoretical lower and upper limits of 0 and 1.
- »Gnaiger 2020 BEC MitoPathways - Chapter 3«
- » Posted by: »Komlodi Timea« and »Cecatto Cristiane«
- Mar-2 Tu
Measuring fatty acid oxidation (FAO)-linked respiration requires a low concentration of malate
In the absence of malate, accumulation of Acetyl-CoA inhibits FAO.
- »Fatty acid oxidation pathway control state«, »Mitochondrial Pathways: Chapter 5«
- communicated by »Cecatto Cristiane« and »Cardoso Luiza«
Jan 2021
- Jan-21 Th
Measuring ATP production with Magnesium Green – which sample preparations work?
Magnesium Green (MgG) can be used with mitochondrial preparations: isolated mitochondria, tissue homogenates, and permeabilized cells and tissues.
- »MgG« and »Publications MgG«
- communicated by »Cardoso Luiza« and »Komlodi Timea«