Difference between revisions of "Template:MitoPediaAlert2021"
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:::::: <big>''Currently Answered Questions from the »[[OROBOROS_INSTRUMENTS|Oroboros Ecosystem Agenda]]« | :::::: <big>''Currently Answered Questions from the »[[OROBOROS_INSTRUMENTS|Oroboros Ecosystem Agenda]]« | ||
</big>__TOC__ | </big>__TOC__ | ||
== May 2021 == | |||
*'''May-11 Tu''' | |||
:{| | |||
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | |||
|''' Measuring succinate pathway (S-pathway) in living cells '''<br> | |||
Using [[MitoKit-CII]] – cell permeable succinate and malonate, the [[Succinate pathway|S-pathway]] can be measured <br> | |||
- »'''[[SUIT-003 O2 ce D060]]'''«<br> | |||
- communicated by »[[Cecatto Cristiane]]« and »[[Komlodi Timea]]« | |||
|} | |||
== Apr 2021 == | |||
== Mar 2021 == | == Mar 2021 == | ||
*'''Mar-16 Tu''' | *'''Mar-16 Tu''' | ||
:{| | :{| |
Revision as of 08:38, 10 May 2021
- Currently Answered Questions from the »Oroboros Ecosystem Agenda«
May 2021
- May-11 Tu
Measuring succinate pathway (S-pathway) in living cells
Using MitoKit-CII – cell permeable succinate and malonate, the S-pathway can be measured
- »SUIT-003 O2 ce D060«
- communicated by »Cecatto Cristiane« and »Komlodi Timea«
Apr 2021
Mar 2021
- Mar-16 Tu
CI inhibitors like rotenone inhibit fatty acid oxidation (FAO)-linked respiration
Inhibition of CI leads to accumulation of NADH in the mt-matrix. The resultant lack of NAD+ blocks FAO.
- »Fatty acid oxidation pathway control state«, »Mitochondrial Pathways: Chapter 5«
- communicated by »Cardoso Luiza« and »Cecatto Cristiane«
- Mar-11 Th
Internal normalization of rate – what is the difference between flux control ratio and flux control efficiency?
A flux control ratio is the ratio of oxygen flux in a specific respiratory control state, normalized for maximum flux in a common reference state. Flux control efficiency expresses the control of respiration as a fractional change of oxygen flux or flow between protocol steps. Both values vary between theoretical lower and upper limits of 0 and 1.
- »Gnaiger 2020 BEC MitoPathways - Chapter 3«
- » Posted by: »Komlodi Timea« and »Cecatto Cristiane«
- Mar-2 Tu
Measuring fatty acid oxidation (FAO)-linked respiration requires a low concentration of malate
In the absence of malate, accumulation of Acetyl-CoA inhibits FAO.
- »Fatty acid oxidation pathway control state«, »Mitochondrial Pathways: Chapter 5«
- communicated by »Cecatto Cristiane« and »Cardoso Luiza«
Jan 2021
- Jan-21 Th
Measuring ATP production with Magnesium Green – which sample preparations work?
Magnesium Green (MgG) can be used with mitochondrial preparations: isolated mitochondria, tissue homogenates, and permeabilized cells and tissues.
- »MgG« and »Publications MgG«
- communicated by »Cardoso Luiza« and »Komlodi Timea«