TIP2k-Module: Difference between revisions
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::* Align the rear ends of the TIP2k and O2k, if you use [[Microsyringe\500 mm3\TIP2k |500 µl syringes]]. | ::* Align the rear ends of the TIP2k and O2k, if you use [[Microsyringe\500 mm3\TIP2k |500 µl syringes]]. | ||
== Technical characteristics == | |||
''Titration:'' | |||
Programmable, automatic titration regimes, with titration volumes of 0.05 to 250 µl, variable titration intervals and duration of titration pulses. | |||
''Injection:'' | |||
Steady-state injection: Operation at quasi steady-states by continuous injection of substrates at limiting rates of consumption, providing new flexibility in experimental design by combining the technical advantages of closed and open systems. Programmable injection flows: 0.01 to 25 µl.s-1. | |||
Ramp injection (MiPNet04.05, see above): Ramp increase of effector concentrations by "continuous titration". | |||
*DatLab software for feedback control by the the TIP2k: for steady-state respirometry at selected oxygen levels and pH-stat applications. | |||
== TIP2k-Syringes == | == TIP2k-Syringes == | ||
Revision as of 12:42, 5 September 2019
TIP2k-Module
| Description | TIP2k-Module - Titration-Injection microPump (TIP2k) for two-channel operation with the O2k-FluoRespirometer with automatic control by DatLab of programmable titration regimes and feedback control (oxystat, pH-stat). The TIP2k-Module includes TIP2k-Accessories: 4 Microinjection syringes, 1 Pkg. TIP2k-Needle Spacers with mounting tool, 1 TIP2k-Needle Safety Support and 1 Pkg. TIP2k Filter Papers, and the newest DatLab version. |
|---|---|
| Product ID | 11100-03 |
| Type | O2k, O2k-Module, Microsyringe, O2k-FluoRespirometer |
| Link | MiPNet12.10 TIP2k-manual, MiPNet04.05 Titration-Injection |
| Image |  |
MitoPedia O2k and high-resolution respirometry:
O2k hardware
TIP2k-Module consists of:
O2k with TIP2k
- » TIP2k-Manual
- The TIP2k is fully supported by the O2k.
The TIP2k is placed at the top of the Oroboros O2k. It does not need any extra bench space.
- Align the front legs of the TIP2k with the TIP2k-Spacer Bar on the front of the O2k if you use 200 µl syringes.
- Align the rear ends of the TIP2k and O2k, if you use 500 µl syringes.
Technical characteristics
Titration: Programmable, automatic titration regimes, with titration volumes of 0.05 to 250 µl, variable titration intervals and duration of titration pulses.
Injection: Steady-state injection: Operation at quasi steady-states by continuous injection of substrates at limiting rates of consumption, providing new flexibility in experimental design by combining the technical advantages of closed and open systems. Programmable injection flows: 0.01 to 25 µl.s-1. Ramp injection (MiPNet04.05, see above): Ramp increase of effector concentrations by "continuous titration".
- DatLab software for feedback control by the the TIP2k: for steady-state respirometry at selected oxygen levels and pH-stat applications.
TIP2k-Syringes
- For larger volumes, the 500 µl syringes are available.
- » SOP: Handling microsyringes
- Rinse the outside with water immediately after use.
- Wash 3 x with last used solvent, rinse with EtOH.
- Storage: ethanol (short), dry (longtime).
- Rinse with pure solvent before use.
- Technical support: TIP2k syringe blocked
