SUIT-003 O2 ce D009: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|description=[[File:1ce;2ceOmy;3ceU-.jpg|150px]] | |description=[[File:1ce;2ceOmy;3ceU-.jpg|150px]] | ||
|info='''A''' [[Huetter 2004 Biochem J]] | |info='''A''' [[Huetter 2004 Biochem J]] |
Revision as of 13:58, 30 January 2018
Description
Reference: A Huetter 2004 Biochem J
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT protocol pattern: linear cell (ce) coupling control protocol (ceCCP)
References
R;L;E format_ROX
- The ceCCP diagram may show the respiratory states directly (left) as an alternative to presenting the titration steps.
- Rot and Ama
- In this ceCCP, ROX is measured after titration of rotenone, antimycin A, or both in a single step. Validity of using rotenone only for inducing ROX in intact cells is evaluated by stepwise measurement of ROX after titration of rotenone (3Rot) followed by titration of animycin A (4ceAma).
1ce;2ceOmy;3ceU;4ceRot;5ceAma
- 1ce;2ceOmy;3ceU;4ceRot;5ceAma
- In this ceCCP, ROX is measured after stepwise titration of rotenone (4ceRot) and antimycin A (5ceAma). In many intact cells ROX measured after titration of rotenone (4ceRot) is not or only slightly further inhibited by subsequent titration of animycin A (5ceAma).
- Respiration in senescent human primary fibroblasts (0.2Γ106 cells/ml). Arrows show steps in the titration regime of the coupling control protocol, inducing the following respiratory states.
- 1ce: ROUTINE (R; routine state in cell-culture medium) in intact cells (ce);
- 2ceOmy: LEAK (L; inhibition of ATPsynthase by 1 ΞΌg/ml oligomycin);
- 3ceU: ET-pathway (E; maximal stimulation by uncoupling of oxidative phosphorylation in four subsequent titrations of the uncoupler, U, FCCP (2.5β4 ΞΌM final concentration);
- 4ceRot: ROX (rotenone, Rot; residual oxygen consumption).
- 5ceAma: ROX (antimycin A, Ama; residual oxygen consumption). Modified after Huetter_2004_Biochem J.
- Respiration in senescent human primary fibroblasts (0.2Γ106 cells/ml). Arrows show steps in the titration regime of the coupling control protocol, inducing the following respiratory states.
- HΓΌtter E, Renner K, Pfister G, StΓΆckl P, Jansen-DΓΌrr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - Β»Bioblast linkΒ«