Oxygen signal

high-resolution terminology - matching measurements at high-resolution

Oxygen signal

Description

The oxygen signal of the OROBOROS O2k is transmitted from the electrochemical polarographic oxygen sensor (OroboPOS) for each of the two chambers to DatLab. The primary signal is a current [mAmp] which is converted into a voltage [V], and calibrated in SI units for amount of substrance concentration [µmol.dm-3 or µM].

Reference: MiPNet19.18D O2k-Series G and DatLab 6: Calibration, MiPNet06.03 POS-calibration-SOP

|mitopedia method=Respirometry }}

MitoPedia O2k and high-resolution respirometry: O2k hardware, DatLab 

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MitoPedia O2k and high-resolution respirometry: O2k-Open Support 

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Stability of the oxygen signal

The stability of the oxygen signal of the O2k is evaluated by the (negative) uncorrected slope over time. After smoothing, the slope of a stable sensor should be less than +- 1 pmol.s-1.ml-1 in a 2-ml chamber.

• Instability of the oxygen signal

• There is drift of the oxygen signal over short or long periods of time.
• The signal at air saturation is abnormally high for a given gain setting.
• The zero current is high (>2.5%) and may show drift.

• Follow the standard instructions for oxygen sensor service.

Oxygen signal does not respond

• The oxygen signal remains at or close to zero even at high oxygen levels.
• The signal at air saturation is abnormally low.

1. Check the gain setting for the affected oxygen channel. Set to Gain 1.
2. Apply technical service: O2k-Open Support