From Bioblast
O2k-Open Support |
- - we provide service and support based on open innovation
MitoPedia
- Find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages, specifically and the O2k and high-resolution respirometry:
- Β» MitoPedia: O2k hardware
- Β» MitoPedia: DatLab
- Β» MitoPedia: SUIT: Substrate-uncoupler-inhibitor-titration protocols
O2k-Protocols
- O2k-Protocols explain various applications of the O2k with O2k-Demo experiments, SOPs beyond the O2k-Manual, and more: Β» O2k-Protocols
Β» O2k-Protocols: MitoPathways | |
Β» O2k-SOP | |
Β» O2k-Demo experiments | |
Β» O2k-Chemicals and media | |
Β» O2k-mitochondrial preparations | |
Β» O2k-Protocols: Mitochondrial and marker-enzymes |
- Implement your quality control management with the MitoFit QCS, to diagnose technical problems fast and cost-effectively with instrumental quality control tests.
- Β» O2k-standard operating procedures: Β» O2k-SOP
- Β» Polarographic oxygen sensors: calibration, accuracy and quality control SOP. Β» MiPNet06.03 POS-calibration-SOP
- Β» Instrumental O2 background correction, accuracy of oxygen flux and SOP. Β» MiPNet14.06 Instrumental O2 background
O2k-Manual
- The O2k-Manual helps beginners and experienced users. Find technical information and instructions in the main sections (Bioblast pdf files), complemented by details on specific webpages: Β» O2k-Manual
O2k-Videosupport
O2k-Catalogue
- The O2k-Catalogue is a guided tour through the O2k technology, with links to specific sections of the O2k-Manual, and more: Β» OROBOROS O2k-Catalogue
Frequently Asked Questions
- Β» OROBOROS FAQ
Open innovation
- Open innovation extends the support given to individual users to the scientific community and provides feedback to improve the scope and documentation of the O2k-technology - in the spirit of Gentle Science.
- We welcome and value feedback. Technical and scientific questions, which are not yet addressed in the available guidelines or are difficult to trace on our website, will be relevant to both specialists and beginners in the field.
- Expansion of O2k-SOPs is particularly relevant for novel approaches in O2k-Fluorometry and O2k-Spectrophotometry, which are combined in the development of the NextGen-O2k.
- Extended O2k-technical support
- O2k-technical support is defined as extended if support is requested on O2k-technology and high-resolution respirometry without prior consulting the O2k-Manual and relevant chapters on O2k-Protocols.
- Regular consultancy fees apply for extended O2k-technical support requested without open innovation agreement.
- Acceptance of extended O2k-technical support with an open innovation agreement implies that OROBOROS INSTRUMENTS may publish the relevant correspondence or a summary of the correspondence addressing technical and scientific questions on our website, including names, institutional and Email addresses.
- Extended O2k-technical support
O2k-technical support
- Application problems
- An application problem can be diagnosed on the basis of the O2 sensor and O2k-chamber tests. If proper instrumental performance is obtained, software and hardware problems are excluded.
- Application problems
- Software problems
- Communication between the O2k and DatLab running on a computer.
- Software problems
- Hardware problems
- Hardware problems may be solved by user-service: In particular, the OroboPOS (polarographic oxygen sensor) requires user-service at intervals (these may be more than 1 year), and SOPs are available to determine if a sensor service is required at an earlier or later date.
- Hardware problems may require replacement of a defective sparepart.
- Some electronic or mechanical defects may be solved only by service of the O2k in the workshop of OROBOROS INSTRUMENTS, e.g. a defective Peltier unit.
- Hardware problems
O2k Quality Control
The fundamental O2k tests
- The O2 sensor and O2k chamber tests are important to eliminate instrumental artefacts (Instrumental quality control: O2k-QC), distinguished from Assay QC addressing problems of solution protocols (perparation of chemicals), sample preparation protocols, and SUIT protocols.
- Biological experiments are not suitable for trouble shooting.
- Clean O2k-Chambers and contamination-free incubation medium are required.
- Analysis of DatLab files recorded in such instrumental tests is essential.
- The O2 sensor and O2k chamber tests are important to eliminate instrumental artefacts (Instrumental quality control: O2k-QC), distinguished from Assay QC addressing problems of solution protocols (perparation of chemicals), sample preparation protocols, and SUIT protocols.
- The sequence of Instrumental QC tests is strictly
- O2 sensor test,
- O2 chamber test.
- The sequence of Instrumental QC tests is strictly
The 2-chamber design of the O2k
- The two chamber design of the O2k has many benefits, including advantages in trouble shooting. By switching components between sides A and B of the O2k, it is usually possible to locate an instrumental problem, finding selectively a specific defective component of the O2k. This component can then be serviced by the end user or replaced by OROBOROS without the need to ship the entire O2k.
O2 sensor test
- Many technical problems of O2k-Core applications can be analyzed and solved with the O2 sensor test, described in detail as an O2k-SOP:
- Β» O2 sensor test: MiPNet06.03 POS-calibration-SOP
O2k-chamber test
Standard operating procedure
- If all quality control criteria of the O2 sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-Chamber assembly has to be tested, described in detail as an O2k-SOP:
- Β» O2k chamber test: Instrumental O2 background
- The O2k-chamber test provides quality control at an instrumental level beyond the O2 sensor test:
- O2k-Chamber not properly assembled or broken.
- OroboPOS-Holder not properly positioned.
- O2k-Chamber Holder not properly positioned; V- and O-rings not properly mounted (V-ring\30-35-4.5 mm, O-ring\Viton\18x2 mm).
- Volume-Calibration Ring not properly positioned by chamber volume calibration.
- O-ring\Viton\12x1 mm injured and must be replaced on the stopper.
- Stopper\black PEEK\conical Shaft\central Port broken conical edge or O-ring not properly applied.
- OroboPOS-Seal Tip leaky.
- Experimental medium consumes oxygen due to microbial contamination.
- O2k-Chamber not properly assembled or broken.
- The O2k-chamber test provides quality control at an instrumental level beyond the O2 sensor test:
Trouble shooting
- If specifications given in the Instrumental O2 background are not obtained: check components for locating the problem.
- Check the stirring bars for any contamination.
- Check the stoppers for the quality of the O-rings and the conical edges.
- If no indications of a defect are observed, disassemble the O2k-chamber.
- Check the glass chamber for contamination or for broken edges.
- Clean the copper block of the O2k and reassemble the O2k-chamber.
- Reassemble and clean the chambers: MiPNet19.03 O2k-cleaning and ISS.
- Perform an O2 sensor test and - if successful - an O2k-chamber test, using fresh incubation medium.
- If the problem with the instrumental O2 background remains in one chamber, switch stoppers between chambers A and B.
- Perform an O2k-chamber test (the sensor test is not necessary).
- If the problem with the instrumental O2 background remains in the same chamber, switch glass chambers between the left and right side of the O2k.
- Perform an O2 sensor test and - if successful - proceed with the O2k-chamber test.
- If the problem with the instrumental O2 background remains in the same chamber, switch sensors between the left and right chamber.
- If specifications given in the Instrumental O2 background are not obtained: check components for locating the problem.
Cost estimation for repair
- A cost estimation for repair of the O2k-Main Unit due to an electronic or mechanical defect can only be given after inspection of the O2k by our workshop in Austria. Once a cost estimation has been provided, the repair is carried out after the customerβs consent. For shipment of the O2k, follow the detailed instructions.
The O2k-Network
- The OROBOROS O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.
- Β» The O2k-Network Labs: Β» O2k-Network
Contact
- Β» In case you need further technical support and for your valuable feedback, please contact [email protected]
- Β» OROBOROS MitoFit Laboratory - open for innovation and cooperation.
- Β» OROROBOS Science Scholarships - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry