Difference between revisions of "MitoPediaAlert2020 Archive"
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| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]] | | width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]] | ||
| ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the [[Electron transfer-pathway |electron transfer system]]. | | ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the [[Electron transfer-pathway |electron transfer system]].<br> | ||
- »'''[[Hatefi 1962 J Biol Chem-XLII]]'''« | - »'''[[Hatefi 1962 J Biol Chem-XLII]]'''« | ||
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* '''Apr-21 Tu''' | * '''Apr-21 Tu''' | ||
[[File:CS.png|right|100px|Citrate synthase]] | [[File:CS.png|right|100px|Citrate synthase]] | ||
:{| | :{| | ||
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]] | | width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]] | ||
| [[Citrate synthase]] activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature. | | [[Citrate synthase]] activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.<br> | ||
- »'''[[MiPNet17.04 CitrateSynthase]]'''« | - »'''[[MiPNet17.04 CitrateSynthase]]'''« | ||
|} | |} | ||
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* '''Apr-13 Mo''' | * '''Apr-13 Mo''' | ||
:{| | :{| | ||
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | | width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | ||
| ''' Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?''' | | ''' Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?'''<br> | ||
DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the [[mitochondrial preparation]]<br> | |||
- »'''[[SUIT-024 |SUIT-024]]'''« | |||
|} | |} | ||
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* '''Apr-08 We''' | * '''Apr-08 We''' | ||
:{| | :{| | ||
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | | width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | ||
| '''The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.''' | | '''The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.'''<br> | ||
- DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum [[digitonin]] concentration. - »'''[[SUIT-010 |SUIT-010]]'''« | - DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum [[digitonin]] concentration.<br> | ||
- »'''[[SUIT-010 |SUIT-010]]'''« | |||
|} | |} | ||
<br /> | <br /> | ||
* '''Apr-02 Th''' | * '''Apr-02 Th''' | ||
:{| | :{| | ||
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | | width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | ||
|'''Do you want to search the best [[SUIT]] protocol for your research questions?''' | |'''Do you want to search the best [[SUIT]] protocol for your research questions?'''<br> | ||
- Use the SUITbrowser: »'''[[SUITbrowser]]'''« | |||
|} | |} | ||
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Revision as of 11:01, 4 May 2020
- Currently Answered Questions from the »Oroboros Ecosystem Agenda«
May 2020
- May-07 Th
Do you know how to evaluate fatty acid oxidation (FAO, F-pathway) avoiding to overestimate it? Did you know that malate alone might support mt-respiration in mitochondrial preparations, if malate anaplerotic pathways (e.g. mt-malic enzyme) are present?
- Learn more about - »malate anaplerotic pathway control state«
- DatLab 7.4 provides a step-by-step guide through the SUIT-027 to evaluate the malate anaplerotic pathway - »SUIT-027«
- Since F-pathway can be overestimated due to anaplerotic reactions, SUIT-002 was specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways - »SUIT-002«
Apr 2020
- Apr-24 Fr
ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the electron transfer system.
- Apr-21 Tu
Citrate synthase activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.
- Apr-13 Mo
Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?
DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the mitochondrial preparation
- »SUIT-024«
- Apr-08 We
The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.
- DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum digitonin concentration.
- »SUIT-010«
- Apr-02 Th
Do you want to search the best SUIT protocol for your research questions?
- Use the SUITbrowser: »SUITbrowser«
Mar 2020
- Mar-16 Mo
The standardized coupling control protocol to explore the bioenergetics of living cells - DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. - »SUIT-003«
- Mar-11 We
Does blebbistatin reduce oxygen dependence of respiration of permeabilized muscle fibers? This is one of the questions addressed in the COST Action CA15203 MitoEAGLE WG2. - »Blebbistatin«
- Mar-06 Fr
Explore metabolic profiles and specific modulations of mitochondrial pathways in health and disease with SUIT reference protocols.
- Mar-03 Tu
Rapamycin - more differences or more in common with other mitObesity drugs? - »Rapamycin«
Feb 2020
- Feb-26 We
It is essential to titrate uncouplers in multiple steps for respirometric measurement of electron transfer capacity.
- Feb-24 Mo
- Feb-19 We
Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function. - »Spermidine«
- Feb-18 Tu
Why do we fight gas bubbles? - Dive deep into »Oxygen solubility«
- Feb-12 We
What do metformin, melatonin and other bioactive mitObesity compounds have in common? - »MitObesity drugs«
Jan 2020
- Jan-16 Th
Oxygen flux - which SI units should be used?
Did you ever think that units for body mass could be an issue? - »Body mass«
- Jan-08 We
Force and Energy: this we all need in the New Year - and MitoPedia may help to clarify the difference.