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...plasmic hybrid cells as a model to characterize the OXPHOS system: control sample with glutamate metabolism defect. ...showed declined effect after addition of glutamate, but overreaction after addition of succinate. Respiratory chain complex activities for both clones of the U

:::* It is recommended to run a chemical background without any sample to test the effect of the chemicals on the fluorescence signal. More inform ...ion about the NS(PGM) pathway in the OXPHOS and ET-states, but without the addition of Omy.

...tion of uncoupler, the required concentration of Omy has to be determined. Addition of Omy results in hyperpolarisation owing to the fact that the inhibition o :::* It is recommended to run a chemical background assay without any sample to test the effect of the chemicals on the fluorescence signal. More inform

...of the mt-membrane, while [[ADP]] (D) decreases the mt-membrane potential. Addition of [[oligomycin]] (Omy) results in hyperpolarisation since the inhibition o Since high concentrations of Omy can decrease the ET capacity induced by addition of uncoupler, the optimal concentration of Omy has to be determined. For mt

::: The 1 M stock solution is used for addition at the beginning of an experiment (SUIT protocol). ...e medium aliquot or directly to the O2k chamber before the addition of the sample. Recommendations based on the following publications:

...ometric (HRR) method. The O2 flux in a whole-cell sample combined with the addition of mitochondrial substrates, uncouplers and inhibitors enabled us to accura

...Since higher concentrations of Omy can decrease the ET state induced upon addition of uncoupler, the required concentration of Omy has to be assessed by the [ ...ated mitochondria, tissue homogenate and cells permeabilized previously to addition to the O2k-chamber.

...of malate anaplerotic pathway due to mitochondrial [[malic enzyme]] in the sample studied. ...he DLP for SUIT-027 O2 ce-pce D065 is provided without the [[antimycin A]] addition step. However, it is possible to create a [[Export DL-Protocol User (*.DLPU

...mpare 27 candidate models predicting (all of which included body mass). In addition to mass, the two best-fitting models (cumulative Akaike weight=0.93) includ

...preparations are utilized, the O2k provides the unique potential to reduce sample size to as little as 1 mg fresh weight per experimental run, supporting app ...er]] of the O2k. Alternatively, cells attached to beads can be used. The [[Sample Holder]] may be useful for these applications. Alternatively, gentle stirri

...l neurons. Mitoplasts were prepared from the sample of the mitochondria by addition to a hypotonic solution to induce swelling following outer membrane rapture

...impler choice since it does not require loading and washing the dye in the sample. ...ted ~70 µM as the optimal EGTA concentration (which can be added after the addition of the cells and before digitonin permeabilization).

...ime. Highest signal stability minimizes the required amounts of biological sample, and provides the basis for resolution in the extreme low-oxygen range. Pel

...ng control in [[mitochondrial preparations |mtprep]] (''see'' [[MitoPedia: Sample preparations]]) at [[Succinate pathway control state]]. ...ation. A [[Export DL-Protocol User (*.DLPU)|DLPU]] can be created with the addition of oligomycin. It must be taken in consideration that high concentrations o

...ustness and reliability of instrumental performance. With small amounts of sample and correspondingly low respiratory flux per volume, the oxygen capacity of ...E'' ratio is 0.10. Respiration is inhibited at higher [FCCP], unrelated to sample dilution (<1 %). O2k-experiment 2005-04-09 P3-03, carried out by participan

...(v) additional information is acquired for a limited amount of sample. In addition, there are practical and economical advantages, saving handling time and mo ...ll. This extends MultiSensor applications, obtaining the optical signal in addition to the signals of the oxygen sensor and of other electrodes inserted throug

...trate synthase activity as marker of mitochondrial content in the measured sample. ...without inhibitory effect. Durability tests demonstrated that heart biopsy sample was stable on ice for up to 48 hours (with huge inter-individual variabilit

...ent of mitochondrial membrane potential and oxygen consumption in the same sample. The method described herein is useful for the study of malignant cells, wh

...Any incubation medium can be used for zero calibration with dithionite or sample. Unlike air calibration, it is not necessary to perform a zero calibration

...sterile distilled water and submitted to 100 mechanical successions. This sample was subjected to mechanical sucussions 100, leading to 1x. This procedure w ...s with a tendency to decrease fluorescence intensity in all treatments. In addition, treatment with nosodes decreased the MA104 cells respiratory capacity.