Oroboros O2k-Core (O2k-Series D - G)

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Oroboros O2k-Core (O2k-Series D - G)

O2k-Catalogue

Description Oroboros O2k-Core (O2k-Series D - G) - the experimental system complete for basic high-resolution respirometry (HRR). The O2k-Core includes the O2k-Main Unit with stainless steel housing, O2k-Assembly Kit, two OroboPOS (polarographic oxygen sensors) and OroboPOS-Service Kit, DatLab software, the ISS-Integrated Suction System and the O2k-Titration Set. The O2k-Core supports all add-on O2k-Modules of the O2k.

On-line display of oxygen flux (rate of respiration) is provided in addition to the conventional 'oxygraphic' plot of oxygen concentration over time. Highest signal stability minimizes the required amounts of biological sample, and provides the basis for resolution in the extreme low-oxygen range. Peltier temperature control provides a thermal stability at ±0.002 °C in the range of 4 °C to 47 °C at typical constant room temperature. Electronically controlled PVDF or PEEK stirrers are integrated in the two-chamber design of the O2k, and a barometric pressure transducer enables automatic oxygen calibrations implemented in the DatLab software.

The O2k is a sole source apparatus with no other instruments meeting its specifications.

Product ID 10000-02
Type Former Series
Link Gnaiger_2008_POS
Image
O2k-Core-Concept.jpg
  • A PC is required (not included), connecting DatLab to the O2k via a USB port. For PC requirements, see here.
Questions.jpg




O2k-Core manual and selected protocols

» Contents: O2k-Core Manual DatLab 6.pdf
ChapterViewSectionLast update
Gnaiger 2008 POSBioblast pdf
O2k-Protocols contents
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.
2008
Gnaiger 2014 MitoPathwaysBioblast pdf
O2k-Protocols
Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. Oroboros MiPNet Publications, Innsbruck:80 pp.
2014
Gnaiger 2019 MitoPathwaysBioblast pdf 5th edition in preparation
O2k-Protocols
Gnaiger E (2019) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Mitochondr Physiol Network 24.05. Oroboros MiPNet Publications, Innsbruck:112 pp.
2019
MiPNet08.09 CellRespirationBioblast pdf  »Versions
O2k-Procedures contents
High-resolution respirometry and coupling control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX.
2016-08-11
MiPNet12.01 Suppl T-issueBioblast pdf »Versions
O2k-Protocols contents
MitoPathways at the Q-junction: mouse skeletal muscle fibers.
2018-11-18
MiPNet12.11 MitoRespirationBioblast pdf »Versions
O2k-Protocols
Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control.
2014-02-20
MiPNet17.03 Shredder vs FibersBioblast pdf »Versions
O2k-Protocols
Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder.
2015-06-18
MiPNet19.18B POS-serviceBioblast pdf »Versions
O2k-Manual
Service of the polarographic oxygen sensor OroboPOS.
2019-05-15
MiPNet21.16 DatLab 7 Innovations
About Oroboros
DatLab 7: innovations.
2019-09-23
Pesta 2012 Methods Mol BiolBioblast pdf
PMID: 22057559
O2k-Protocols
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58.
2012
Oxygraph-2k-Concept.jpg


MitoPedia O2k and high-resolution respirometry: O2k hardware