Tunholi 2016 J Invertebr Pathol: Difference between revisions
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{{Publication | {{Publication | ||
|title=Tunholi VM, Tunholi-Alves VM, Santos AT, Garcia Jda S, Maldonado A Jr, da-Silva WS, Rodrigues Mde L, Pinheiro J (2016) Evaluation of the mitochondrial system in the gonad-digestive gland complex of ''Biomphalaria glabrata'' (Mollusca, Gastropoda) after infection by ''Echinostoma paraensei'' (Trematoda, Echinostomatidae). J Invertebr Pathol 136:136-41. | |title=Tunholi VM, Tunholi-Alves VM, Santos AT, Garcia Jda S, Maldonado A Jr, da-Silva WS, Rodrigues Mde L, Pinheiro J (2016) Evaluation of the mitochondrial system in the gonad-digestive gland complex of ''Biomphalaria glabrata'' (Mollusca, Gastropoda) after infection by ''Echinostoma paraensei'' (Trematoda, Echinostomatidae). J Invertebr Pathol 136:136-41. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/27079167 PMID: 27079167] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/27079167 PMID: 27079167] | ||
|authors=Tunholi VM, Tunholi-Alves VM, Santos AT, Garcia Jda S, Maldonado A Jr, da-Silva WS, Rodrigues Mde L, Pinheiro J | |authors=Tunholi VM, Tunholi-Alves VM, Santos AT, Garcia Jda S, Maldonado A Jr, da-Silva WS, Rodrigues Mde L, Pinheiro J | ||
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{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration | ||
| | |organism=Molluscs | ||
|tissues=Genital | |tissues=Genital | ||
|preparations=Permeabilized tissue | |preparations=Permeabilized tissue | ||
|couplingstates=LEAK, OXPHOS, | |couplingstates=LEAK, OXPHOS, ET | ||
| | |pathways=N, NS, ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional= | |additional=2016-06 | ||
}} | }} | ||
Latest revision as of 16:19, 13 November 2017
| Tunholi VM, Tunholi-Alves VM, Santos AT, Garcia Jda S, Maldonado A Jr, da-Silva WS, Rodrigues Mde L, Pinheiro J (2016) Evaluation of the mitochondrial system in the gonad-digestive gland complex of Biomphalaria glabrata (Mollusca, Gastropoda) after infection by Echinostoma paraensei (Trematoda, Echinostomatidae). J Invertebr Pathol 136:136-41. |
Tunholi VM, Tunholi-Alves VM, Santos AT, Garcia Jda S, Maldonado A Jr, da-Silva WS, Rodrigues Mde L, Pinheiro J (2016) J Invertebr Pathol
Abstract: The effect of infection by Echinostoma paraensei on the mitochondrial physiology of Biomphalaria glabrata was investigated after exposure to 50 miracidia. The snails were dissected one, two, three and four weeks after infection for collection and mechanical permeabilization of the gonad-digestive gland (DGG) complex. The results obtained indicate that prepatent infection by this echinostomatid fluke significantly suppresses the phosphorylation state (respiratory state 3) and basal oxygen consumption of B. glabrata, demonstrating that the infection reduces the ability of the intermediate host to carry out aerobic oxidative reactions. Additionally, relevant variations related to the uncoupled mitochondrial (state 3u) of B. glabrata infected by E. paraensei were observed. Four weeks after exposure, a significant reduction in mitochondrial oxygen consumption after addition of ADP (3.68±0.26pmol O2/mg proteins) was observed in the infected snails in comparison with the respective control group (5.14±0.25). In the uncoupled state, the infected snails consumed about 62% less oxygen than the infected snails (7.87±0.84pmol O2/mg proteins) in the same period. These results demonstrate a reduction in oxidative decarboxylation rate of the tricarboxylic acid cycle and faster anaerobic degradation of carbohydrates in the infected snails. The possible mechanisms that explain this new metabolic condition in the infected organisms are discussed.
Copyright © 2016 Elsevier Inc. All rights reserved. • Keywords: Biomphalaria glabrata, Echinostoma paraensei, Host-parasite relationship, Mitochondrial metabolism
Labels: MiParea: Respiration
Organism: Molluscs
Tissue;cell: Genital
Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS, ET
Pathway: N, NS, ROX
HRR: Oxygraph-2k
2016-06
