Template:SUIT text D027: Difference between revisions
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The SUIT-012 O2 mt D027 protocols specifically focus on assessing the linear coupling control ([[LEAK-respiration|''L'']]-[[Oxidative phosphorylation| ''P'']]-[[ET-capacity| ''E'']]) with NADH-linked substrates ([[PM pathway control state|PM]]) in [[Mitochondrial preparations| mitochondrial preparations]] such as isolated mitochondria, tissue homogenates and permeabilized cells (already permeabilized when they are added to the chamber) in a wide variety of | The SUIT-012 O2 mt D027 protocols specifically focus on assessing the linear coupling control ([[LEAK-respiration|''L'']]-[[Oxidative phosphorylation| ''P'']]-[[ET-capacity| ''E'']]) with NADH-linked substrates ([[PM pathway control state|PM]]) in [[Mitochondrial preparations| mitochondrial preparations]] such as isolated mitochondria, tissue homogenates and permeabilized cells (already permeabilized when they are added to the chamber) in a wide variety of organisms and tissues. Addition of [[Glutamate|G]] in NADH-supported [[Oxidative phosphorylation| OXPHO]]S enables evaluating the [[glutamate anaplerotic pathway control state]]. SUIT-012 O2 mt D027 can be extended with the CIV assay module. | ||
Revision as of 16:47, 19 July 2019
The SUIT-012 O2 mt D027 protocols specifically focus on assessing the linear coupling control (L- P- E) with NADH-linked substrates (PM) in mitochondrial preparations such as isolated mitochondria, tissue homogenates and permeabilized cells (already permeabilized when they are added to the chamber) in a wide variety of organisms and tissues. Addition of G in NADH-supported OXPHOS enables evaluating the glutamate anaplerotic pathway control state. SUIT-012 O2 mt D027 can be extended with the CIV assay module.
