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Latest revision as of 09:37, 21 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

Next Week

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 15
-* '''Apr-08 Mo'''
+Week 21
-:::: 16:30 '''[[Gnaiger Erich|Erich Gnaiger]] presenting a [[MOSBRI Consensus Meeting 2024 Rome IT|MOSBRI Consensus Meeting]] satellite seminar at O2k-Network Lab [[IT Rome Giuffre A]]''' - ''Unlocking cell metabolism: Exploring the power of high-resolution respirometry with the NextGen-O2k''
-----
+* '''May-20 Mo'''
-* '''Apr-09 Tu'''
-:::: 10:00 '''[[Gnaiger Erich|Erich Gnaiger]] presenting at [[MOSBRI Consensus Meeting 2024 Rome IT]]''' - ''Quality-controlled high-resolution respirometry: bacterial and clinical applications''
-----
-* '''Apr-10 We'''
 :{|
 | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' Is fatty acid oxidation (FAO) considered as CI- or CII-driven respiration? '''<br>
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
-Neither. There is simultaneous electron transfer from FAO into the Q-junction through the electron transferring flavoprotein dehydrogenase complex (CETFDH) and Complex I. <br>
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
-- »'''[[Fatty acid oxidation]]'''«, »'''[[Gnaiger 2020 BEC MitoPathways]]'''«, »'''[[Gnaiger 2024 J Biol Chem]]'''« <br>
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
-- communicated by »[[Timon-Gomez Alba]]« and »[[Willis Jaime R]]«
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
 |}
+----
+* '''May-21 Tu'''
 ----
-* '''Apr-11 Th'''
+* '''May-22 We''' - International Day for Biological Diversity
-World Parkinson's Disease Day
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"The markers of mitochondrial biogenesis were consistently affected in PD [Parkinson’s disease] patients group."''' <br>
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-* Kamienieva I, Charzyńska A, Duszyński J, Malińska D, Szczepanowska J (2023) In search for mitochondrial biomarkers of Parkinson's disease: Findings in parkin-mutant human fibroblasts. [[Kamienieva 2023 Biochim Biophys Acta Mol Basis Dis|»Bioblast link«]]
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
-:from the O2k-Network »[[PL Warsaw Szewczyk A]]«
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
 |}
+----
+* '''May-23 Th'''
 ----
-* '''Apr-12 Fr'''
+* '''May-24 Fr'''
 ----
 '''<big>Next Week</big>'''
-Week 16
+Week 22
-* '''Apr-15 Mo'''
+* '''May-27 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|'''Do you observe either a zero or low raw O<sub>2</sub> signal when starting a measurement?'''<br>
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
-Perform a test to evaluate whether this is related to an improperly positioned chamber or a faulty connection between the OroboPOS and the OroboPOS-Connector. <br>
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
-- »'''[https://wiki.oroboros.at/index.php/Oxygen_signal#Raw_signal Oxygen signal]'''«<br>
+<br>
-- communicated by »[[Grings Mateus]]« and »[[Schmitt Sabine]]«
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-* '''Apr-16 Tu'''
+* '''May-28 Tu'''
 ----
-* '''Apr-17 We'''
+* '''May-29 We'''
 ----
-* '''Apr-18 Th'''
+* '''May-30 Th'''
 :{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|''' How to store the POS overnight and up to several months? '''<br>
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-Fill the chamber and the receptacle of the loosely inserted stopper with 70 % EtOH, add the Cover-slip and switch off the O2K. <br>
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
-- »'''[[MiPNet19.03 O2k-cleaning and ISS]]'''«<br>
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
-- communicated by »[[Leo Elettra]]« and »[[Grings Mateus]]«
 |}
 ----
-* '''Apr-19 Fr'''
+* '''May-31 Fr'''
 ----