Template:Current week ecosystem agenda: Difference between revisions

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  2021 Week 49
  2024 Week 20
 
*'''Dec-06 Mo'''


* '''May-13 Mo'''
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
|'''Do you see fluctuations of O<sub>2</sub> slope negative following a pattern similar to the Peltier power, but without block temperature variation?'''<br>
Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O<sub>2</sub> slope negative.
<br>
- »'''[[Oxygen_flux_-_instrumental_background#Fluctuations_of_O2_slope_negative|Fluctuations of O<sub>2</sub> slope negative]]'''«<br>
- communicated by »[[Cardoso Luiza]]« and »[[Grings Mateus]]«
|}
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* '''May-14 Tu'''
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*'''Dec-07 Tu'''
* '''May-15 We'''  
:{|
:{|
| width="110" align="center" | [[File:Gnaiger 2020 BEC MitoPathways.jpg|70px|link=https://www.bioenergetics-communications.org/index.php/bec/article/view/gnaiger_2020_mitopathways|Gnaiger 2020 BEC MitoPathways]]
| width="110" align="center" | [[File:Tributes to pioneers icon.jpg|left|80px|BEC Tributes to pioneers in bioenergetics|link=Tributes to pioneers in bioenergetics]]
|'''What is ROUTINE respiration?'''<br>
|Azzi A, Gnaiger E (2024) Tributes to pioneers in bioenergetics - wandering in the garden of memories. Bioenerg Commun 2024.3.v1. https://doi.org/10.26124/bec:2024-0003<br>
ROUTINE respiration is the respiratory activity of living (non-permeabilized) cells in the physiological coupling state, which is controlled by cellular energy demand, energy turnover and the degree of coupling to phosphorylation. <br>
- [[Azzi_2024_BEC-Tributes |»Bioblast link«]]
- »[https://www.bioenergetics-communications.org/index.php/bec/article/view/gnaiger_2020_mitopathways Gnaiger 2020 BEC MitoPathways - Chapter 2.4]« <br>
- communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]«
|}
|}
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*'''Dec-08 We'''
* '''May-16 Th'''  
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''The central role of the Q-junction in mitochondria'''<br>
|''' "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."'''<br>
The Q-junction is a point of convergence in the electron transfer system. Electrons flow from different dehydrogenases and respiratory Complexes into the Q-junction and then downstream to Complex III. <br>
- »'''[[MiPNet27.07 Sample Holder]]'''«<br>
- »[[Q-junction]]« <br>
- communicated by »[[Willis Jaime R]]« and »[[Timon-Gomez Alba]]«
- communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]« <br>
|}
|}
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*'''Dec-09 Th'''
* '''May-17 Fr'''
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'''<big>Next Week</big>'''
 
2024 Week 21
 
* '''May-20 Mo'''  
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|'''How do you measure ROUTINE respiration?'''<br>
|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
ROUTINE respiration (''R'') of living cells is measured before permeabilization of the cell membrane either in cell culture medium or any other respiration media (e.g. MiR05) in the presence or absence of exogenous substrates. Allow ''R'' to stabilize for 15-20 min. <br>
Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
- »[[ROUTINE respiration]]« and »[[Doerrier 2018 Methods Mol Biol]]« <br>
- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
- communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]«
- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
|
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* '''May-21 Tu'''  
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* '''May-22 We''' - International Day for Biological Diversity
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
:from the O2k-Network »[[NO Tromsoe Wood SH]]«
|}
|}
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* '''May-23 Th'''
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* '''May-24 Fr'''
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*'''Dec-10 Fr'''

Latest revision as of 08:23, 15 May 2024

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A future for Ukraine is the future of its science
2024 Week 20
  • May-13 Mo
O2k-Open SupportInfo: take care Do you see fluctuations of O2 slope negative following a pattern similar to the Peltier power, but without block temperature variation?

Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O2 slope negative.
- »Fluctuations of O2 slope negative«
- communicated by »Cardoso Luiza« and »Grings Mateus«


  • May-14 Tu

  • May-15 We
BEC Tributes to pioneers in bioenergetics
Azzi A, Gnaiger E (2024) Tributes to pioneers in bioenergetics - wandering in the garden of memories. Bioenerg Commun 2024.3.v1. https://doi.org/10.26124/bec:2024-0003

- »Bioblast link«


  • May-16 Th
O2k-Open SupportInfo: improve "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."

- »MiPNet27.07 Sample Holder«
- communicated by »Willis Jaime R« and »Timon-Gomez Alba«


  • May-17 Fr

Next Week

2024 Week 21
  • May-20 Mo
MitoPedia
How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test« 
- communicated by »Baglivo Eleonora« and »Schmitt Sabine« 


  • May-21 Tu

  • May-22 We - International Day for Biological Diversity
O2k-Publications in the MiPMap "We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."
  • Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«
from the O2k-Network »NO Tromsoe Wood SH«

  • May-23 Th

  • May-24 Fr

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