Talk:Raw signal of the oxygen sensor: Difference between revisions
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== DatLab 8 == | == DatLab 8 == | ||
[[File:DL8_status_line_arrows.png|thumb|right|800px|Raw data displayed in the status bar]] | |||
[[File:DL8_help_O2k_communication_log.png|thumb|right|200px|O2k communication log in the top menu bar]] | [[File:DL8_help_O2k_communication_log.png|thumb|right|200px|O2k communication log in the top menu bar]] | ||
::; Raw signal display | ::; Raw signal display | ||
:::: The control panel at the very bottom of the measurement window shows the raw signal values. | :::: The control panel at the very bottom of the measurement window shows the raw signal values. |
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Talk:Raw signal of the oxygen sensor |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Description
The raw signal of the polarographic oxygen sensor (POS) reflects the number of O2 molecules reacting with the sensor. Calibration of the raw signal with the known air oxygen concentration at a given barometric pressure and temperature allows for exact measurement of the oxygen concentration in the O2k chamber. See O2k-Support page: Oxygen signal. Observation of the raw signal values is important for quality control and trouble-shooting procedures.
Abbreviation: R
Reference: MiPNet06.03 POS-calibration-SOP, MiPNet12.20_O2k-calibration_tutorial
DatLab 8
- Raw signal display
-
- The control panel at the very bottom of the measurement window shows the raw signal values.
- In the top menu bar select 'Help > O2k communication log' OR
- Press Ctrl+L to access the communication log and find all values sent from the instrument to DatLab.
- Typical values at air saturation
-
- At air oxygen concentration the raw signal should be close to 2 ยตA (values between 1 and 3 ยตA are acceptable at temperatures between 25 and 37 ยฐC and altitudes up to 1000 m above sea level).
- Typical values at zero oxygen
-
- After O2 removal from the O2k chamber by dithionite or oxygen consuming biological samples, the raw signal should be stable and < 2% of the signal at air saturation, but < 5% is acceptable.
- A raw signal of more than 5 % of air saturation level can usually be solved by a sensor service