Search results

• D-number
  ...l membrane potential|Fluo]], [[MgG]]). Since the use of different kinds of sample or application may result in slightly different steps, each protocol receiv ...ises different related protocols, each specifically designed for different sample preparations:
  2 KB (251 words) - 14:32, 5 August 2019
• Permeabilized tissue
  '''Addition of sample to the respirometer chamber:''' For permeabilized tissue, remove the Stoppe |mitopedia topic=Sample preparation
  951 bytes (115 words) - 17:08, 26 May 2020
• Markova 2016 Abstract MitoFit Science Camp 2016
  ...reparation from experimental pigs with minimal mitochondrial damage in the sample and sufficient penetration of the substrates utilized during respirometry. ...was inserted into a Falcon tube with ice-cold BIOPS. Approximately 2mg of sample tissue were cut off on ice from the left ventricle and were processed by tw
  3 KB (450 words) - 15:25, 23 January 2019
• Sample
  ...ation is improved, if the symbol ''N'' is used for the number of [[primary sample]]s taken from a study group, and the symbol ''n'' is used for the number of |mitopedia topic=Sample preparation
  6 KB (956 words) - 16:48, 21 February 2024
• O2k-Virtual Support self-study material
  '''Sample types,mitochondrial preparations and sample addition:''' :::* [[Sample]]
  5 KB (754 words) - 12:50, 7 November 2023
• Select plots - DatLab
  | ''c'') In addition, on correction for instrumental O₂ background flux; edit in [Flu | ''d'') In addition, on information about sample concentration; edit [F3]: Million cells.
  5 KB (769 words) - 15:28, 11 April 2018
• Schinagl 2016 PLoS One
  ...y. The degree of inhibition strongly depended on the sequence of inhibitor addition. This suggested, as postulated for plants, that the alternative terminal ox
  3 KB (386 words) - 15:46, 14 October 2023
• SUIT-026 O2 mt D063
  ...dyes may inhibit components of the ET system) and quality control for the sample for [[SUIT-026 AmR mt D064]]. ...a_analysis|mt outer membrane integrity]] provides a quality control of the sample, which cannot be performed in the protocol with Amplex UltraRed.
  2 KB (254 words) - 00:01, 13 April 2021
• Mark statistics - DatLab
  ...f the previous layout was displaying the O2 slope negative, the calculated sample-specific flux will not be automatically background corrected. To obtain the ...tion, exported data include information contained in the [[Sample_-_DatLab|Sample window]], information contained in the [[O2_calibration_-_DatLab#Air_calibr
  6 KB (916 words) - 20:54, 11 June 2023
• Razak 2018 RUMeS
  ...P) uncoupler. Residual oxygen consumption (ROX) was obtained following the addition of complex I inhibitor (rotenone) and complex III inhibitor (antimycin A). ...aningful information regarding the mitochondrial bioenergetics states of a sample.
  2 KB (322 words) - 11:47, 8 April 2019
• Flux analysis - DatLab
  == Smoothing, noise, and sample stability == ...hibitor. Flux may increase over time due to (''1'') a slow response to the addition of ADP or a fuel substrate, or (''2'') an undershoot observed in rare cases
  4 KB (640 words) - 11:46, 25 March 2022
• Permer 2017 Bachelor Thesis
  ...f the fact in which laboratory the measurement has been performed. Such as sample has to be stable for a certain amount of time and has to deliver the same r ...titration protocol, the use of intact HEK293T cells for a stable reference sample appears to be a promising possibility.
  3 KB (456 words) - 16:46, 23 January 2019
• Pacheco 2008 Master Thesis
  muscle from the same biopsy sample. The purpose of this study is to test the functional capacity of mitochondr ...fresh muscle tissue. The significant increase in state 3 respiration upon addition of cytochrome c indicates that cryopreservation causes results in cytochrom
  2 KB (285 words) - 09:40, 8 November 2016
• SUIT-026 O2 ce-pce D088
  ...dyes may inhibit components of the ET system) and quality control for the sample for [[SUIT-026 AmR ce-pce D088]]. ...a_analysis|mt outer membrane integrity]] provides a quality control of the sample, which cannot be performed in the protocol with Amplex UltraRed.
  2 KB (296 words) - 12:04, 5 September 2022
• SUIT-034 NADH mt D082
  ...dized [[NAD]] can be obtained due to low ADP (0.1 μM) titration before the addition of fuel substrates in contrast to [[SUIT-006 NADH mt D084]]. :::- When ATPases are present in the sample, it is not possible to reach the [[LEAK]] state.
  3 KB (454 words) - 16:56, 21 December 2023
• SUIT-033 NADH mt D081
  ...dized [[NAD]] can be obtained due to low ADP (0.1 μM) titration before the addition of fuel substrates in contrast to [[SUIT-032 NADH mt D078]] or [[SUIT-006 N :::- When ATPases are present in the sample, it is not possible to reach the [[LEAK]] state.
  3 KB (462 words) - 17:44, 4 January 2024
• Ost 2018 Curr Opin Clin Nutr Metab Care
  ...s mitochondrial respiratory function in tissue biopsies or blood cells. In addition, it summarizes the recent literature related to this topic. ...tandard operating procedures are required regarding instrumental settings, sample collection and preparation, protocol design and respirometric data analysis
  2 KB (311 words) - 14:40, 5 July 2023
• SUIT-018 AmR mt D040
  ...is to reduce the oxygen concentration in the O2k-Chamber before the sample addition. To decrease or increase the oxygen concentration in the O2k-Chamber, see [ ...ary reoxygenations (high sample concentration) or very long protocols (low sample concentration causing slower oxygen consumption in the O2k-Chamber and thus
  4 KB (625 words) - 12:09, 7 March 2024
• Rhodamine 123
  ...should be tested for their effect in a background run without a biological sample, and the necessary corrections are applied. ...The fluorescence signal was calibrated by Rhodamine 123 before adding the sample in a step-wise titration (max. concentration 1 µM). The optimum concentrat
  5 KB (698 words) - 15:50, 16 March 2021
• Krajcova 2018 MiPschool Tromso E2
  ...ue biopsy (isolated mitochondria) or lengthy and time-consuming process of sample preparation (permeabilized muscle fibers). In this study we aimed to develo ...CP and 4 µM antimycin. The technique requires only 20 mg of myocardium and sample preparation takes less than 20 min.
  3 KB (460 words) - 23:10, 19 October 2018
• Cytochrome c
  ...cy]]). The following DatLab traces illustrate examples of cytochrome ''c'' addition: ...flux per volume (red trace, right axis) can be seen upon cytochrome ''c'' addition ([[Cytochrome c control efficiency|cytochrome ''c'' control efficiency]]=0.
  6 KB (844 words) - 09:37, 4 April 2024
• SUIT-031 Q mt D072
  ...rotenone does not have a further effect on the Q-signal in the presence of sample and CoQ₂, this test can be omitted. ...or isolated mitochondria, tissue homogenate and cells permeabilized before addition to the O2k- chamber.
  4 KB (530 words) - 16:17, 13 December 2023
• SUIT-031 Q ce-pce D074
  ...rotenone does not have a further effect on the Q signal in the presence of sample and [[coenzyme Q2]], this test can be omitted. ...or isolated mitochondria, tissue homogenate and cells permeabilized before addition to the O2k- chamber.
  4 KB (519 words) - 17:52, 13 December 2023
• Schoepf 2014 Abstract MiP2014
  ...ial response to cellular stress exerted by enhanced ROS production [4]. In addition, an easy and fast assay for determination of CIV activity was applied addin ...d in MiR05 with creatine (MiR05Cr) at 37 °C. Parallel analyses of a paired sample consisting of one benign and one malign biopsy from a single patient could
  7 KB (962 words) - 18:31, 10 January 2022
• Setting the oxygen concentration
  ...or decreasing oxygen concentration during an experiment with a biological sample is relevant for specific applications (''e.g.'', hypoxia, tissue normoxia, ...rted to O₂ with a high catalase concentration able to avoid any sample oxidative stress. During this procedure the O2k-chamber remains closed, dec
  5 KB (828 words) - 17:10, 31 January 2024
• Cytochrome c control efficiency
  .... https://doi.org/10.26124/bec:2020-0002</ref>, ''j''_{cyt ''c''}. Addition of [[cytochrome c| '''cytochrome ''c''''']] (10 µM final concentration)<re ...wo values to calculate the increase of respiration due to cytochrome ''c'' addition.
  11 KB (1,496 words) - 11:35, 21 January 2022
• SUIT-007
  .... If [[Glutamate dehydrogenase|glutamate dehydrogenase]] is present in the sample, when glutamate alone is added, it will be converted to α-ketoglutarate in ...the mitochondrial external membrane as it would be with GM. However, with addition of cytochrome ''c'' only after malate (e.g. 1G;2D;3M;3c), it would not be p
  3 KB (401 words) - 11:30, 3 June 2020
• Kane 2016 Abstract MitoFit Science Camp 2016
  ...ore, be to introduce contraction inhibitor early in the preparation of the sample. ...ated with myosin II ATPase inhibitor blebbistatin (bleb) during the entire sample preparation exhibit different JO2 when assayed in standard MiR06 sucrose re
  6 KB (790 words) - 17:46, 7 November 2016
• Steady state
  ..., since the increase in the oxygen flux is not due to the cytochrome ''c'' addition, but instead because flux increases further over time (see Figure 2 for mor ...ed by the straight line (from 2D to 2c) is not due to the cytochrome ''c'' addition. More time would have been requred to reach a stable flux before step 2c. C
  8 KB (1,115 words) - 03:00, 23 January 2023
• O2k-Videosupport
  ...j5GfuUwDI ]] [https://www.youtube.com/watch?v=NJj5GfuUwDI Insertion of the Sample Holder into the 2.0 mL O2k-chamber] ...Xgg ]] [https://www.youtube.com/watch?v=GX_SvnVGXgg Addition of biological sample to the respirometer chamber]
  9 KB (1,435 words) - 12:42, 14 November 2023
• SUIT-006 02 mt D108
  ...ed after the experiment to avoid carry-over of inhibitors. The addition of sample homogenate is recommended in the washing protocol to bind strong inhibitors
  2 KB (233 words) - 10:43, 26 January 2024
• Krajcova 2020 PLOS ONE
  ...d integrity of outer mitochondrial membrane (increase of respiration after addition of cytochrome ''c'' is up to 11.7±1.8 % vs. 15.7±3.1 %, ''p''˂0.05 and 1
  3 KB (373 words) - 03:31, 18 April 2024
• SUIT-034 O2 mt D111
  :::- When ATPases are present in the sample, it is not possible to reach the [[LEAK]] state. ...after the experiment to avoid carry-over of uncoupler and inhibitors. The addition of liver homogenate is recommended in the washing protocol to bind strong i
  2 KB (244 words) - 09:56, 26 January 2024
• SUIT-026 AmR ce-pce D087
  ...D022]] for quality control and a full assessment of the coupling control. Addition of [[Antimycin A|Ama]] leads to increased ROS production via inhibition of ...nd_data_analysis|mt outer membrane integrity]] as a quality control of the sample).
  4 KB (562 words) - 13:52, 31 August 2023
• SUIT-026 AmR mt D077
  ...D022]] for quality control and a full assessment of the coupling control. Addition of [[Antimycin A|Ama]] leads to increased ROS production via inhibition of ...nd_data_analysis|mt outer membrane integrity]] as a quality control of the sample).
  4 KB (575 words) - 12:06, 31 August 2023
• SUIT-033 O2 mt D110
  :::- When ATPases are present in the sample, it is not possible to reach the [[LEAK]] state. ...ng is required after the experiment to avoid carry-over of inhibitors. The addition of liver homogenate is recommended in the washing protocol to bind strong i
  2 KB (238 words) - 09:57, 26 January 2024
• Jiroutkova 2014 Intensive Care Medicine Experimental
  ...ely. Integrity of outer membrane was assessed by measuring the response to addition of cytochrome c. Oxygen consumption rate was normalized to citrate-synthase
  3 KB (402 words) - 12:09, 9 December 2021
• SUIT-026 AmR mt D064
  ...D022]] for quality control and a full assessment of the coupling control. Addition of [[Antimycin A|Ama]] leads to increased ROS production via inhibition of ...nd_data_analysis|mt outer membrane integrity]] as a quality control of the sample).
  4 KB (609 words) - 00:00, 13 April 2021
• Stelfa 2018 MiP2018
  ...viously [1]. To induce anoxia, respiration of sample was stimulated by the addition of succinate with rotenone and ADP and preparation was left to consume all ...sub>2</sub>O₂/O flux ratio after anoxia-reoxygenation. Overall, addition of R-phenibut prevented anoxia-reoxygenation-induced increase in H<sub>2</s
  4 KB (521 words) - 12:48, 17 March 2019
• MitoEAGLE protocols, terminology, documentation
  ...al for large-scale production. For respiratory OXPHOS analysis a reference sample of structurally and functionally intact mitochondria is not yet available t ...ides the ability to make diagnostic profiling associated with diseases. In addition to functional data, other data types such as nutritional or environmental f
  11 KB (1,507 words) - 12:05, 19 January 2018
• Kidere 2018 MiPschool Tromso E2
  ...plasmic hybrid cells as a model to characterize the OXPHOS system: control sample with glutamate metabolism defect. ...showed declined effect after addition of glutamate, but overreaction after addition of succinate. Respiratory chain complex activities for both clones of the U
  4 KB (630 words) - 12:22, 17 October 2018
• SUIT-020 Fluo mt D033
  :::* It is recommended to run a chemical background without any sample to test the effect of the chemicals on the fluorescence signal. More inform ...ion about the NS(PGM) pathway in the OXPHOS and ET-states, but without the addition of Omy.
  5 KB (655 words) - 08:41, 30 September 2020
• SUIT-021 Fluo mt D036
  ...tion of uncoupler, the required concentration of Omy has to be determined. Addition of Omy results in hyperpolarisation owing to the fact that the inhibition o :::* It is recommended to run a chemical background assay without any sample to test the effect of the chemicals on the fluorescence signal. More inform
  6 KB (834 words) - 14:07, 6 September 2021
• SUIT-006 Fluo mt D034
  ...of the mt-membrane, while [[ADP]] (D) decreases the mt-membrane potential. Addition of [[oligomycin]] (Omy) results in hyperpolarisation since the inhibition o Since high concentrations of Omy can decrease the ET capacity induced by addition of uncoupler, the optimal concentration of Omy has to be determined. For mt
  4 KB (623 words) - 14:08, 6 September 2021
• Magnesium Green
  ::: The 1 M stock solution is used for addition at the beginning of an experiment (SUIT protocol). ...e medium aliquot or directly to the O2k chamber before the addition of the sample. Recommendations based on the following publications:
  17 KB (2,616 words) - 15:42, 7 February 2024
• Aidt 2013 PLoS Curr
  ...ometric (HRR) method. The O2 flux in a whole-cell sample combined with the addition of mitochondrial substrates, uncouplers and inhibitors enabled us to accura
  2 KB (312 words) - 21:21, 17 March 2019
• SUIT-006
  ...Since higher concentrations of Omy can decrease the ET state induced upon addition of uncoupler, the required concentration of Omy has to be assessed by the [ ...ated mitochondria, tissue homogenate and cells permeabilized previously to addition to the O2k-chamber.
  8 KB (1,169 words) - 13:08, 13 December 2023
• SUIT-027 O2 ce-pce D065
  ...of malate anaplerotic pathway due to mitochondrial [[malic enzyme]] in the sample studied. ...he DLP for SUIT-027 O2 ce-pce D065 is provided without the [[antimycin A]] addition step. However, it is possible to create a [[Export DL-Protocol User (*.DLPU
  2 KB (310 words) - 00:01, 13 April 2021
• Dlugosz 2013 J Exp Biol
  ...mpare 27 candidate models predicting (all of which included body mass). In addition to mass, the two best-fitting models (cumulative Akaike weight=0.93) includ
  2 KB (324 words) - 11:31, 11 December 2019
• Oroboros FAQ
  ...preparations are utilized, the O2k provides the unique potential to reduce sample size to as little as 1 mg fresh weight per experimental run, supporting app ...er]] of the O2k. Alternatively, cells attached to beads can be used. The [[Sample Holder]] may be useful for these applications. Alternatively, gentle stirri
  7 KB (949 words) - 11:36, 8 November 2023
• Kajma MiP2010
  ...l neurons. Mitoplasts were prepared from the sample of the mitochondria by addition to a hypotonic solution to induce swelling following outer membrane rapture
  3 KB (381 words) - 21:14, 18 March 2018
• Calcium Green
  ...impler choice since it does not require loading and washing the dye in the sample. ...ted ~70 µM as the optimal EGTA concentration (which can be added after the addition of the cells and before digitonin permeabilization).
  7 KB (971 words) - 15:33, 2 June 2022
• Oroboros O2k-Core (O2k-Series D - G)
  ...ime. Highest signal stability minimizes the required amounts of biological sample, and provides the basis for resolution in the extreme low-oxygen range. Pel
  3 KB (389 words) - 11:45, 14 April 2021
• SUIT-006 O2 mt D022
  ...ng control in [[mitochondrial preparations |mtprep]] (''see'' [[MitoPedia: Sample preparations]]) at [[Succinate pathway control state]]. ...ation. A [[Export DL-Protocol User (*.DLPU)|DLPU]] can be created with the addition of oligomycin. It must be taken in consideration that high concentrations o
  3 KB (368 words) - 23:53, 12 April 2021
• Gnaiger 2008 POS
  ...ustness and reliability of instrumental performance. With small amounts of sample and correspondingly low respiratory flux per volume, the oxygen capacity of ...E'' ratio is 0.10. Respiration is inhibited at higher [FCCP], unrelated to sample dilution (<1 %). O2k-experiment 2005-04-09 P3-03, carried out by participan
  5 KB (638 words) - 00:30, 5 March 2022
• Fasching 2011 Abstract Berlin
  ...(v) additional information is acquired for a limited amount of sample. In addition, there are practical and economical advantages, saving handling time and mo ...ll. This extends MultiSensor applications, obtaining the optical signal in addition to the signals of the oxygen sensor and of other electrodes inserted throug
  4 KB (583 words) - 18:18, 10 January 2022
• Krajcova 2017 MITOEAGLE Obergurgl
  ...trate synthase activity as marker of mitochondrial content in the measured sample. ...without inhibitory effect. Durability tests demonstrated that heart biopsy sample was stable on ice for up to 48 hours (with huge inter-individual variabilit
  6 KB (806 words) - 04:58, 18 March 2019
• Chinopoulos 2014 Methods Enzymol
  ...ent of mitochondrial membrane potential and oxygen consumption in the same sample. The method described herein is useful for the study of malignant cells, wh
  3 KB (420 words) - 11:49, 27 October 2023
• Zero calibration
  ...Any incubation medium can be used for zero calibration with dithionite or sample. Unlike air calibration, it is not necessary to perform a zero calibration
  3 KB (502 words) - 10:20, 5 April 2024
• Homsani 2013 Int J High Dilution Res
  ...sterile distilled water and submitted to 100 mechanical successions. This sample was subjected to mechanical sucussions 100, leading to 1x. This procedure w ...s with a tendency to decrease fluorescence intensity in all treatments. In addition, treatment with nosodes decreased the MA104 cells respiratory capacity.
  3 KB (417 words) - 09:22, 9 November 2016
• Pinho 2020 PLoS Negl Trop Dis
  ...iles for glucose uptake and O₂ consumption in these species. In addition, we provide quantitative data about different membrane proteins, transporte
  3 KB (405 words) - 18:17, 6 November 2020
• SUIT-026
  ...ing site of the [[Complex I]] occurs, promoting the production of ROS. The addition of [[rotenone]] provides excellent control to this mechanism because this c ...nd_data_analysis|mt outer membrane integrity]] as a quality control of the sample.
  5 KB (809 words) - 12:39, 24 January 2024
• Pesta 2011Abstract Mitochondrial Medicine-Diagnosis
  ...on in the presence of glutamate and subsequent rescue of respiration after addition of malate. A small needle biopsy sample was obtained from the patient´s M. vastus lateralis to assess mitochondria
  4 KB (585 words) - 18:17, 10 January 2022
• Gnaiger 2015 Abstract MiP2015
  ...drial function of cyropreserved HEK 293T cells: development of a reference sample for high-resolution respirometry. ...ment ([[MitoEAGLE]]). A QMSmtf requires the availability of a mt-reference sample which is functionally stable over time and across geographical space, as a
  6 KB (848 words) - 18:32, 10 January 2022
• Dahdah 2019 MiP2019
  ...ease of fatty acids and/or triglycerides when permeabilizing the cells. In addition, normalization of the samples is different between protocols and between th
  3 KB (451 words) - 14:39, 3 October 2019
• Flux / Slope
  ...ll-down menu for (1) normalization of flux (chamber volume-specific flux, sample-specific flux or flow, or flux control ratios), (2) [[flux baseline correct ...nstrumental standard Layouts 1-3, which display the O2 slope negative, the sample-specific flux or flow, or flux control ratios will not be automatically bac
  17 KB (2,751 words) - 09:55, 17 November 2023
• Fasching 2012 Abstract Bioblast
  ...ll. This extends MultiSensor applications, obtaining the optical signal in addition to the signals of the oxygen sensor and of other electrodes inserted throug
  5 KB (636 words) - 18:21, 10 January 2022
• Cell count and normalization in HRR
  ...alance in the 2-mL O2k-chamber when the stopper is removed ('''O'''ff) for addition of a cell suspension with partial volume replacement PVR. ...alance in the 2-mL O2k-chamber when the stopper is removed ('''O'''ff) for addition of a cell suspension with complete volume replacement CVR.
  35 KB (5,607 words) - 16:01, 21 February 2024
• NextGen-O2k Documentation
  [https://www.youtube.com/watch?v=GX_SvnVGXgg Addition of biological sample to the respirometer chamber]
  5 KB (760 words) - 13:23, 3 April 2022
• Timon-Gomez 2023 MiP2023
  ...be used as a general diagnostic tool for bioenergetic profiling in various sample preparations from different cell types, tissues, and organisms.<br>
  4 KB (456 words) - 16:47, 12 July 2023
• Sumbalova 2016a Abstract MitoFit Science Camp 2016
  ...al approach while at the same time minimizing experimental variability and sample requirement. Nevertheless, there are some inherent limitations to this appr ...oxaloacetate [2], which may not be added directly but may be produced upon addition of other substrates [3]. Observation on multiple types of mitochondria reve
  7 KB (1,014 words) - 19:16, 10 January 2022
• Toogood 2004 J Biol Chem
  ...e redox active FAD domain. Together, these enable the FAD domain of ETF to sample a range of conformations, some compatible with fast interprotein electron t ...on metabolism are structurally and evolutionarily distinct enzymes (5). In addition, the membrane-bound ETF-ubiquinone oxidoreductase is unrelated to either of
  4 KB (505 words) - 18:26, 6 September 2023
• SUIT-016
  ...overestimation in the presence of [[Anaplerosis|anaplerotic]] pathways. In addition, the pathway control of [[FN]] and [[FNS]] in [[Oxidative phosphorylation|O ...that normally do not present mitochondrial outer membrane damage after the sample preparation, e.g. human permeabilized muscle fibers.
  4 KB (543 words) - 15:19, 8 June 2020
• Hasli 2015 Master Thesis
  ...itochondria tissue homogenization with Shredder PSI-HRR for preparation of sample was used and tested against Biops with/Saponin, which dissolves cell membra
  4 KB (564 words) - 14:33, 13 November 2017
• Pereira 2015 Abstract MiPschool London 2015
  ...te that HK2 dissociation has no effect on mitochondrial respiration (± ADP addition) regardless of the perfusion protocol. Moreover, HK2 depletion alone does n
  4 KB (479 words) - 12:09, 27 March 2018
• TMRM
  ...should be tested for their effect in a background run without a biological sample, and the necessary corrections are applied. ::::* [[TMRM]] was added 20-30 s before the addition of imt to the O2k chamber.
  17 KB (2,405 words) - 07:29, 5 April 2024
• Chabi 2017 MITOEAGLE Obergurgl
  ...eak respiration rate (saponin concentration and permeabilization time). In addition, RCRPMS variability was the lowest using the optimized conditions determine ..., saponin concentration and incubation time), (ii) calculating the optimal sample size (n) needed to observe statistically significant differences between tw
  4 KB (507 words) - 15:43, 12 January 2018
• Yepez 2018 PLOS One
  ...10 % - 15 %, and that the minimum number of well replicates per biological sample in a 96-well plate should be 12. ...–32]. This analysis also suggests to seed at least 12 wells per biological sample per plate, since we observed increased standard deviations of the residuals
  7 KB (1,035 words) - 13:41, 19 March 2023
• Garcia-Roves 2017 MiP2017
  ...tained could serve as “reference values” for permeabilized skeletal muscle sample preparations. The idea is that new researchers in the field could perform t ...succinate (S); ADP (D*): add 2.5 mM to check if ADP is saturated after the addition of succinate; FCCP/CCCP (U*): 0.5 µM steps to reach the maximum respiratio
  4 KB (557 words) - 16:57, 8 August 2019
• Elementary entity
  ...r of principle, be realized independently at any place and at any time. In addition, new and superior realizations may be introduced as science and technologie ...tity mass. For volume and mass it needs to be ensured that there is a pure sample of oxygen (''X'' = oxygen), but the specification of the ''elementary'' ent
  12 KB (1,989 words) - 13:52, 6 November 2022
• Amplex UltraRed
  ...5 µM. Optimize the final AmR concentration according to respiration media, sample type and concentration, and experimental protocol. These determine the tota ...ium, (DTPA), HRP and Amplex® UltraRed to the O2k-chamber but before sample addition.
  35 KB (5,265 words) - 15:10, 25 April 2024
• MitoFit-Bloodcells
  ...eloped for the separation of different blood cell types from a whole blood sample, allowing the individual functional study of thrombocytes, granulocytes, mo ...monitoring the success of training or therapeutic interventions (WP 7). In addition, biomarkers in blood samples are commonly monitored for risk evaluation (gl
  5 KB (672 words) - 13:27, 3 April 2022
• MitoPediaAlert2020 Archive
  ...ize-independent quantity where the rate is expressed per mass or volume of sample. <br> |''' How can you reduce the amount of biological sample needed for high-resolution respirometry? '''<br>
  21 KB (2,889 words) - 13:31, 19 January 2021
• Permeabilized muscle fibers
  ...P kinetics is fundamentally different in pfi and imt (Saks et al 1988). In addition, oxygen kinetics is shifted by a 100-fold increase of the ''p''_{50</sub === Addition of fibers to the O2k-chamber ===
  23 KB (3,156 words) - 08:57, 18 May 2022
• Tetraphenylphosphonium
  ...isolated mitochondria or cell cultures can be used as biological cleaning sample, which can be stored at -20°C. ::::# Alternatively, the electrodes can be washed with biological cleaning sample added to the O2k-chamber, cleaning the insterted electrodes and the O2k-cha
  37 KB (5,710 words) - 15:30, 16 May 2023
• Garcia-Roves 2018 MiP2018b
  ...nce values for mitochondrial respirometry in permeabilized skeletal muscle sample preparations. The idea is that new researchers in the field follow a refere ...succinate (S); ADP (D*): add 2.5 mM to check if ADP is saturated after the addition of succinate; FCCP/CCCP (U*): 0.5 µM steps to reach the maximum non-couple
  5 KB (735 words) - 12:55, 21 February 2023
• O2k-OpenSupportAlert2020 Archive
  ...nding on which instrumental quality control or measurement with biological sample is performed. Learn more about Standard layouts provided in DatLab: ...ub>2}O₂ calibrations are performed before and after sample addition, and after selected titration steps. <br> <br>
  27 KB (4,026 words) - 11:53, 29 March 2021
• Respirometry
  ...of 1 hour are typical again in [[high-resolution respirometry]], with low sample concentrations and the option of reoxygenations. ...remains controversial up-to-date in discussions of the Warburg effect. In addition, some confusion is caused by the recent literature when no clear distinctio
  11 KB (1,600 words) - 11:15, 12 October 2022
• Oxygen calibration - DatLab
  :::: '''Step <span style="background:red">5</span>''': In addition, the calibration parameter for air calibration, zero oxygen calibration, or ...stirring the aqueous medium in contact with air in the O2k-chamber without sample.
  26 KB (3,995 words) - 10:15, 7 October 2021
• Garcia-Roves 2019 MiPschool Coimbra
  ...nce values for mitochondrial respirometry in permeabilized skeletal muscle sample preparations. The goal is that new researchers in the field follow a refere ...te (3G); succinate (4S; add 2.5 mM ADP to check if ADP is saturating after addition of succinate); FCCP/CCCP (5U*): 0.5 µM titration steps to reach maximum no
  7 KB (913 words) - 11:07, 3 July 2019
• Doerrier 2018 MiP2018c
  ...tion of myosin-ATPase [3]. However, diverse experimental procedures (e.g., sample preparation, protocols, media, and oxygen regimes) render inter- and intra- ...2 mM malate (LEAK state) and saturating ADP with MgCl2 (OXPHOS state). The addition of 10 µM cytochrome c was employed for evaluation of the outer mitochondri
  7 KB (952 words) - 19:05, 10 January 2022
• MitoEAGLE MC Meeting 2021
  ...armonization of protocols across research groups and to compare protocols (sample preparation, experimental details) ...with their knowledge and promote the agenda of MitoEAGLE even further. In addition, we are able to welcome numerous universities and other institutions as mem
  29 KB (4,066 words) - 11:09, 16 March 2021
• Karavaeva Iuliia
  ...ermogenesis. After analyzing the metabolomics data obtained from these two sample sets, we expect to have a short list of potential substrates of the transpo ...ing Slc25a34 will have very low if any respiration increase in response to addition of the physiological substrate of the transporter in comparison to the wild
  12 KB (1,689 words) - 16:20, 16 March 2020
• National Academies of Sciences, Engineering, and Medicine 2017 Fostering integrity in research
  ...the reported work, and all authors should approve the final manuscript. In addition to specifying all authors, standards should (1) provide for the identificat ...legitimately interpreted as a malfunctioning instrument or a contaminated sample. However, true scientific integrity requires the disclosure of the exclusio
  12 KB (1,652 words) - 18:44, 21 April 2024
• K-Regio MitoFit
  ::::# [[MitoPedia: Sample preparations]] ...ochondrial respirometry will be developed, from standardized protocols and sample preparation to the world-wide innovation of a proficiency ring test. These
  43 KB (5,966 words) - 07:05, 29 March 2024
• Oxygen flux - instrumental background
  ...strumental background experiment, measuring oxygen flux without biological sample at four oxygen levels (left), and linear relation between instrumental back :::# Addition of permeabilized fibers into the O2k-chamber: » [[Permeabilized muscle fib
  22 KB (3,238 words) - 15:29, 2 May 2024
• Number
  ...annot count a dot that is not there. Therefore, zero cannot be a count. In addition, zero does not count: ''N''_''X'' + 0 x = ''N''_''X''. ..., the two occurrences have to be expressed in the same quantity and units. Addition requires an identity of quantity and units. Back to the [[MitoFit 2020.4 |'
  33 KB (5,353 words) - 16:10, 28 April 2024
• O2k-pH ISE-Module
  ...higher, or a previous O2k-Series with electronic MultiSensor upgrading. In addition to the two polarographic (amperometric) oxygen channels, the O2k provides t ...ical sample hast to be small as compared to pH changes expected from the sample. The drift will be different in different media (buffering capacity), so
  37 KB (5,713 words) - 12:13, 28 February 2023
• MitoEAGLE Task Group States and rates
  ...ls of an organism, contribute to cell respiration, ''J''_rO2. In addition to mitochondrial catabolic respiration, O₂ is consumed by: (1) M ...n of mitochondrial superoxide formation and the bypass of proton pumps. In addition, the use of protonophores represents an experimental uncoupling interventio
  34 KB (4,911 words) - 11:25, 1 September 2021
• MiPschool Tromso-Bergen 2018
  :::::: (a) reproducibility of sample preparations; ...''I would like to acknowledge the organizers for such a good programme. In addition, the proximity between researchers was very high. Everyone would talk about
  16 KB (2,242 words) - 21:23, 10 January 2022

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