Schaefer 2016 Abstract ADFLIM 2016: Difference between revisions
(Created page with "{{Abstract |title=Mitochondr Physiol Network 21.01. |year=2016 |event=IOC109 }} {{Labeling}}") |
No edit summary |
||
| Line 1: | Line 1: | ||
{{Abstract | {{Abstract | ||
|title= | |title=ADFLIM in AD research –imaging mitochondrial function in Alzheimer´s disease | ||
|authors=Schaefer PM, Einem B von, Niederschweiberer M, Kalinina S, Walther P, Calzia E, Rueck A, Arnim CAF von | |||
|year=2016 | |year=2016 | ||
|event= | |event=ADFLIM 2016 | ||
|abstract=Mitochondrial dysfunction is known as an early feature of Alzheimer´s disease (AD). Amyloid beta (Aβ) as well as its precursor protein APP were identified as key players provoking these mitochondrial disturbances. This entails an energy imbalance in the brain, being one trigger of neuronal death in Alzheimer´s disease. | |||
To further elucidate the role of the intracellular localization of both proteins in mitochondrial impairment, we performed metabolic characterizations of intact cells overexpressing the respective proteins. Using high-resolution respirometry and electron microscopy, we demonstrate especially the intracellular/mitochondrial pool of Aβ to lower mitochondrial respiration. | |||
As the toxic potential of intracellular Aβ underlines the rational of a selective vulnerability of different cell types to Aβ-induced mitochondrial defects, we established a multimodal optical system to measure cell metabolism on the single cell level. Relying on NADH fluorescence lifetime imaging microscopy (NADH FLIM), here we demonstrate that our optical metabolic imaging system is able to mirror the results obtained in the OROBOROS Oxygraph-2k and in surplus displays subcellular resolution representing mitochondrial and neuronal heterogeneity in AD. | |||
Our results demonstrate the importance of assessing energy metabolism on the single cell level to shed light onto Alzheimer´s disease associated mitochondrial dysfunction, highlighting the potential of NADH FLIM for metabolic characterization. | |||
}} | }} | ||
{{Labeling}} | {{Labeling | ||
|area=Respiration | |||
|tissues=Nervous system | |||
|preparations=Intact cells | |||
|injuries=Mitochondrial disease | |||
|diseases=Alzheimer's | |||
|instruments=Oxygraph-2k | |||
}} | |||
== Affiliations == | |||
1-Inst Neurology, 2-Central Facility Electron Microscopy, 3-Inst Anästhesiolog Pathophysiol Verfahrensentwicklung, 4-Core Facility Confocal Multiphoton Microscopy; Ulm University, Germany. - [email protected] | |||
Revision as of 16:49, 10 October 2016
| ADFLIM in AD research –imaging mitochondrial function in Alzheimer´s disease |
Link:
Schaefer PM, Einem B von, Niederschweiberer M, Kalinina S, Walther P, Calzia E, Rueck A, Arnim CAF von (2016)
Event: ADFLIM 2016
Mitochondrial dysfunction is known as an early feature of Alzheimer´s disease (AD). Amyloid beta (Aβ) as well as its precursor protein APP were identified as key players provoking these mitochondrial disturbances. This entails an energy imbalance in the brain, being one trigger of neuronal death in Alzheimer´s disease.
To further elucidate the role of the intracellular localization of both proteins in mitochondrial impairment, we performed metabolic characterizations of intact cells overexpressing the respective proteins. Using high-resolution respirometry and electron microscopy, we demonstrate especially the intracellular/mitochondrial pool of Aβ to lower mitochondrial respiration. As the toxic potential of intracellular Aβ underlines the rational of a selective vulnerability of different cell types to Aβ-induced mitochondrial defects, we established a multimodal optical system to measure cell metabolism on the single cell level. Relying on NADH fluorescence lifetime imaging microscopy (NADH FLIM), here we demonstrate that our optical metabolic imaging system is able to mirror the results obtained in the OROBOROS Oxygraph-2k and in surplus displays subcellular resolution representing mitochondrial and neuronal heterogeneity in AD.
Our results demonstrate the importance of assessing energy metabolism on the single cell level to shed light onto Alzheimer´s disease associated mitochondrial dysfunction, highlighting the potential of NADH FLIM for metabolic characterization.
Labels: MiParea: Respiration Pathology: Alzheimer's Stress:Mitochondrial disease
Tissue;cell: Nervous system Preparation: Intact cells
HRR: Oxygraph-2k
Affiliations
1-Inst Neurology, 2-Central Facility Electron Microscopy, 3-Inst Anästhesiolog Pathophysiol Verfahrensentwicklung, 4-Core Facility Confocal Multiphoton Microscopy; Ulm University, Germany. - [email protected]
