Salicylhydroxamic acid: Difference between revisions
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::: '''SHAM: Salicylhydroxamic acid''' (99%; HOC<sub>6</sub>H<sub>4</sub>CONHOH); Sigma S 607, 5 g, store at RT; M = 153.14 gยทmol<sup>-1</sup>. | ::: '''SHAM: Salicylhydroxamic acid''' (99%; HOC<sub>6</sub>H<sub>4</sub>CONHOH); Sigma S 607, 5 g, store at RT; M = 153.14 gยทmol<sup>-1</sup>. | ||
::: SHAM is reported to be easily soluble in EtOH. So EtOH could be considered as a solvent, too. However, in Oroboros-lab SHAM in EtOH has not been tested yet. | :::: SHAM is reported to be easily soluble in EtOH. So EtOH could be considered as a solvent, too. However, in Oroboros-lab SHAM in EtOH has not been tested yet. | ||
::::<span style="color:#8B008B"> '''Caution:''' Irritant!</span> | ::::<span style="color:#8B008B"> '''Caution:''' Irritant!</span> |
Revision as of 13:13, 3 March 2021
Description
Salicylhydroxamic acid (SHAM; synonym: 2-Hydroxybenzohydroxamic acid N,2-Dihydroxybenzamide) is an inhibitor of the alternative oxidase (AOX). When AOX is blocked by SHAM, electrons are forced through the CIII-cytochrome c oxidase pathway, allowing observation of the operation of the CIII-CIV pathway without AOX activity.
Abbreviation: SHAM
Reference: Parrino_2000_J_Exp_Biol
Application in HRR
- SHAM: Salicylhydroxamic acid (99%; HOC6H4CONHOH); Sigma S 607, 5 g, store at RT; M = 153.14 gยทmol-1.
- SHAM is reported to be easily soluble in EtOH. So EtOH could be considered as a solvent, too. However, in Oroboros-lab SHAM in EtOH has not been tested yet.
- Caution: Irritant!
- Preparation of 250 mM stock solution (dissolved in DMSO):
- Weigh 76.57 mg of SHAM.
- Dissolve in 1950 ยตL of DMSO.
- Divide into 0.2 mL portions
- Store at -20 ยฐC.
- Preparation of 250 mM stock solution (dissolved in DMSO):
- ยป O2k Manual Titrations: MiPNet09.12 O2k-Titrations
- Titration volume: 8 ยตL
- Final conc. in 2 mL O2k chamber: 1 mM
MitoPedia topics:
Inhibitor