SUIT reference protocol: Difference between revisions
No edit summary |
No edit summary |
||
(23 intermediate revisions by 6 users not shown) | |||
Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|abbr=SUIT | |abbr=SUIT RP | ||
|description=The '''substrate-uncoupler-inhibitor titration ([[SUIT]]) reference protocol''', SUIT | |description=The '''substrate-uncoupler-inhibitor titration ([[SUIT]]) reference protocol''', SUIT RP, provides a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories, for establishing a database on comparative mitochondrial phyisology. The SUIT RP consists of two [[harmonized SUIT protocols]] ([[SUIT-001]] - RP1 and [[SUIT-002]] - RP2). These are coordinated such that they can be statistically evaluated as replicate measurements of [[cross-linked respiratory states]], while additional information is obtained when the two protocols are conducted in parallel. Therefore, these harmonized SUIT protocols are complementary with their focus on specific respiratory coupling and pathway control aspects, extending previous strategies for respirometrc OXPHOS analysis. | ||
: SUIT-RP1: | : [[SUIT-001]] (RP1): 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd | ||
: SUIT-RP2: | : [[SUIT-002]] (RP2): 1D;2OctM;2c;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd | ||
|info=[[MiPNet21.06 SUIT | |info=[[Doerrier 2018 Methods Mol Biol]], [[MiPNet21.06 SUIT RP]] | ||
}} | }} | ||
{{MitoPedia concepts | {{MitoPedia concepts | ||
|mitopedia concept=SUIT concept | |||
|mitopedia | |||
}} | }} | ||
[[File:SUIT-catg FNSGpCIV.jpg|right|300px|SUIT-catg]] | |||
[[File: | |||
== Cross-linked respiratory states == | == Cross-linked respiratory states == | ||
The cross-linked respiratory states for harmonization between protocols are: | :::: The cross-linked respiratory states for harmonization between protocols are: | ||
* [[SUIT- | ::::* [[SUIT-001]]: 8Gp, [[SUIT-002]]: 8Rot - [[SGp]]<sub>''E''</sub> | ||
* | ::::* CIV - [[Complex IV single step |Tm]]<sub>''E''</sub> | ||
---- | ---- | ||
[[File:SUIT-Reference-Protocol.jpg| | [[File:SUIT-Reference-Protocol.jpg|500px |SUIT RP]] | ||
<br /> | |||
---- | ---- |
Latest revision as of 10:38, 29 July 2022
Description
The substrate-uncoupler-inhibitor titration (SUIT) reference protocol, SUIT RP, provides a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories, for establishing a database on comparative mitochondrial phyisology. The SUIT RP consists of two harmonized SUIT protocols (SUIT-001 - RP1 and SUIT-002 - RP2). These are coordinated such that they can be statistically evaluated as replicate measurements of cross-linked respiratory states, while additional information is obtained when the two protocols are conducted in parallel. Therefore, these harmonized SUIT protocols are complementary with their focus on specific respiratory coupling and pathway control aspects, extending previous strategies for respirometrc OXPHOS analysis.
- SUIT-001 (RP1): 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
- SUIT-002 (RP2): 1D;2OctM;2c;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd
Abbreviation: SUIT RP
Reference: Doerrier 2018 Methods Mol Biol, MiPNet21.06 SUIT RP
MitoPedia concepts:
SUIT concept