SUIT protocol library

CI - CI+CII - CII

GM+D+S+F+Rot

1. GM+D+c+S+F+Rot: cyt c added after ADP (Boushel 2007 Diabetologia)
2. GM+D+S+c+F+Rot: cyt c added after S

• ADP conc:
• ROX: Ama (Boushel 2007 Diabetologia)

GM+D+P+S+F+Rot

1. Innsbruck protocol 1: GM+D+c+P+S+F+Rot:

• Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E) - ROX
• Cyt c: early addition of c ensures comparability of all states in case of any c effect
• (Gnaiger_2007_MitoPathways)

PM+D+G+S+F+Rot

1. Innsbruck protocol 2: PM+D+c+G+S+F+Rot

• Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E) - ROX
• (Gnaiger_2007_MitoPathways)

PMG+D+S+F+Rot

1. PMG+D+c+S+F+Rot

• Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E)

• ADP conc: in mouse brain homogenate a final conc. of 2 mM ADP in the OXPHOS state (CI+CII) is saturating (Sumbalova_2011_Abstract)
• ROX: Mna+Ama; Myx (Gnaiger_2009_IJBCB)

ETF - CI - CI+CII - CII

1. Greenland-Monte Rosa protocol: OctM+D+G+S+Rot+Omy+F

• Respiratory states: ETF(L) - ETF(P) - CI(P) - CI+II(P) - CII(P) - CII(L) - CII(E) - ROX
• Cyt c: added after F. The absence of a significant c-effect shows the integrity of the outer mitochondrial membrane after fibre preparation and also shows the preservation of integrity over a 90-100 min incubation in the O2k (MiR06).
• Oxygen: oxygen limitation of flux was preventd by maintaining oxygen levels in the respirometer above air saturation (Pesta_2011_Protocols)
• ROX: Mna+Ama
• (Gnaiger_2007_MitoPathways)

Abbreviations

• List_of_respiratory_states
• List_of_terms_and_abbreviations