SUIT-022: Difference between revisions
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::: '''[[SUIT protocol pattern]]:''' linear ce1;ce2KCN;ce3SHAM | ::: '''[[SUIT protocol pattern]]:''' linear ce1;ce2KCN;ce3SHAM | ||
The SUIT-022 is designed to provide an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on [[microalgal cells]] on [[ROUTINE]]. To obtain a complete estimation, both [[SUIT-022 O2 ce D00#]] and [[SUIT-022 O2 ce D00#]] must be carried out in paralel. ย | The SUIT-022 is designed to provide an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on [[microalgal cells]] on [[ROUTINE]]. To obtain a complete estimation, both [[SUIT-022 O2 ce D00#]] and [[SUIT-022 O2 ce D00##]] must be carried out in paralel. ย | ||
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Revision as of 17:24, 4 April 2019
Description
Reference: A: Determination of the respiration due to the alternative oxidase pathway ยปVersions
O2k-Application: O2
- SUIT-category:
- SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM
The SUIT-022 is designed to provide an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on microalgal cells on ROUTINE. To obtain a complete estimation, both SUIT-022 O2 ce D00# and SUIT-022 O2 ce D00## must be carried out in paralel.
Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-04)
Specific SUIT protocols
SUIT-022 O2 ce D00#
- SUIT-022 O2 ce D00# for intact microalgal cells (ce)
SUIT-022 O2 ce D00##
- SUIT-022 O2 ce D00## for for intact microalgal cells (ce)
Strengths and limitations
- SUIT-022 provides an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on microalgal cells on ROUTINE
- + This protocol can be used with other type of cells, although optimal concentrations of inhibitors have to be optimised.
- + Short duration of experiment
- - Estimation of the linear coupling control is not done
- - The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.
Compare SUIT protocols
References
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry