SUIT-003 O2 ce D009: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=CCP_R 1Omy 2U | |abbr=CCP_R,1Omy,2U- | ||
|description=[[File: | |description=[[File:CCP_02.jpg|150px]] | ||
|info=[[MiPNet08.09 CellRespiration]], [[Huetter 2004 Biochem J]] | |info=[[MiPNet08.09 CellRespiration]], [[Huetter 2004 Biochem J]] | ||
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== CCP_02a == | == CCP_02a == | ||
:::* CCP_R 1Omy 2U 3Rot 4Ama | :::* CCP_R,1Omy,2U,3Rot-4Ama | ||
:::* '''Rot and Ama''' Β | :::* '''Rot and Ama''' Β | ||
:::: In this CCP, ROX is measured after stepwise titration of [[rotenone]] (3Rot) and [[antimycin A]] (4Ama). In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A (4Ama). | :::: In this CCP, ROX is measured after stepwise titration of [[rotenone]] (3Rot) and [[antimycin A]] (4Ama). In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A (4Ama). |
Revision as of 20:49, 16 September 2016
Description
Abbreviation: CCP_R,1Omy,2U-
Reference: MiPNet08.09 CellRespiration, Huetter 2004 Biochem J
MitoPedia concepts:
SUIT protocol
- SUIT-catg: CCP
- SUIT protocol pattern: linear coupling control protocol (CCP)
- Mark names in DatLab: CCP_02
- DatLab-Excel template: CCP_02.xlsx
- The CCP diagram may show the respiratory states directly (left) as an alternative to presenting the titration steps.
- Rot and Ama
- In this CCP, ROX is measured after titration of rotenone, antimycin A, or both in a single step. Validity of using rotenone only for inducing ROX in intact cells is evaluated by stepwise measurement of ROX after titration of rotenone (3Rot) followed by titration of animycin A (4Ama).
CCP_02a
- CCP_R,1Omy,2U,3Rot-4Ama
- Rot and Ama
- In this CCP, ROX is measured after stepwise titration of rotenone (3Rot) and antimycin A (4Ama). In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A (4Ama).
- Respiration in senescent human primary fibroblasts (0.2Γ106 cells/ml). Arrows show steps in the titration regime of the coupling control protocol, inducing the following respiratory states: ROUTINE (R; routine state in cell-culture medium); LEAK (L; inhibition of ATPsynthase by 1 ΞΌg/ml oligomycin); ETS (E; maximal stimulation by uncoupling of oxidative phosphorylation in four subsequent titrations of the uncoupler, U, FCCP (2.5β4 ΞΌM final concentration); ROX (rotenone, Rot, and antimycin A; residual oxygen consumption). Modified after Huetter_2004_Biochem J.
- HΓΌtter E, Renner K, Pfister G, StΓΆckl P, Jansen-DΓΌrr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - Β»Bioblast linkΒ«