SUIT-003 O2 ce D009: Difference between revisions
From Bioblast
No edit summary |
No edit summary |
||
Line 2: | Line 2: | ||
|abbr=CC_R 1Omy 2U 3Ama | |abbr=CC_R 1Omy 2U 3Ama | ||
|description=[[File:Ce R 1Omy 2U 3RotAma.png|right|150px]] | |description=[[File:Ce R 1Omy 2U 3RotAma.png|right|150px]] | ||
'''[[Categories of SUIT protocols |SUIT-catg]]:''' | '''[[Categories of SUIT protocols |SUIT-catg]]:''' CCP | ||
|info=[[MiPNet08.09 CellRespiration]] | |info=[[MiPNet08.09 CellRespiration]] | ||
}} | }} | ||
Line 9: | Line 9: | ||
}} | }} | ||
::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP) | ::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP) | ||
::: '''[[Mark names in DatLab]]:''' | ::: '''[[Mark names in DatLab]]:''' CCP_01 | ||
::: '''[[DatLab-Excel templates |DatLab-Excel template]]:''' [[Media:SUIT MiPNet08.09 CellRespiration.xlsx| | ::: '''[[DatLab-Excel templates |DatLab-Excel template]]:''' [[Media:SUIT MiPNet08.09 CellRespiration.xlsx|CCP_01.xlsx]] | ||
:::* '''Rot and Ama''' ย | :::* '''Rot and Ama''' ย | ||
:::: In this CCP, ROX is measured after titration of [[rotenone]], [[antimycin A]], or both in a single step. Validity of using [[rotenone]] only for inducing ROX in intact cells is evaluated by stepwise measurement of ROX after titration of rotenone (3Rot) followed by titration of animycin A (4Ama). | :::: In this CCP, ROX is measured after titration of [[rotenone]], [[antimycin A]], or both in a single step. Validity of using [[rotenone]] only for inducing ROX in intact cells is evaluated by stepwise measurement of ROX after titration of rotenone (3Rot) followed by titration of animycin A (4Ama). | ||
::::ยป ''Further details'': [[CCP_02]], Ce_R 1Omy 2U 3Rot 4Ama | ::::ยป ''Further details'': [[CCP_02]], Ce_R 1Omy 2U 3Rot 4Ama |
Revision as of 20:04, 31 August 2016
Description
SUIT-catg: CCP
Abbreviation: CC_R 1Omy 2U 3Ama
Reference: MiPNet08.09 CellRespiration
MitoPedia concepts:
SUIT protocol
- SUIT protocol pattern: linear coupling control protocol (CCP)
- Mark names in DatLab: CCP_01
- DatLab-Excel template: CCP_01.xlsx
- Rot and Ama
- In this CCP, ROX is measured after titration of rotenone, antimycin A, or both in a single step. Validity of using rotenone only for inducing ROX in intact cells is evaluated by stepwise measurement of ROX after titration of rotenone (3Rot) followed by titration of animycin A (4Ama).
- ยป Further details: CCP_02, Ce_R 1Omy 2U 3Rot 4Ama