SF6847: Difference between revisions

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SF6847

Description

SF 6847 (C₁₈H₂₂N_2O), also known as tyrphostin A9 or malonoben, is a protonophore and a very potent uncoupler of oxidative phosphorylation, being used in the nM range. Like all uncouplers, SF 6847 concentrations must be titrated carefully to evaluated the optimum concentration for maximum stimulation of mitochondrial respiration, particularly to avoid inhibition of respiration at higher concentrations.

Abbreviation: SF6847

Reference: Chinopoulos 2014 Methods Enzymol, Terada 1984 Biochim Biophys Acta

MitoPedia topics: Uncoupler

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 |info=[[Chinopoulos 2014 Methods Enzymol]], [https://www.ncbi.nlm.nih.gov/pubmed/6498178 Terada 1984 Biochim Biophys Acta]
 }}
+{{MitoPedia topics
-== Application in [[HRR]] ==
+|mitopedia topic=Uncoupler
+}}
-=== Preparation ===
-:::: '''SF 6847'''/tyrphostin A9/malonoben (C<sub>18</sub>H<sub>22</sub>N<sub>2O</sub>, [[3,5-bis(1,1-Dimethylethyl)-4-hydroxyphenyl]methylene]propanedinitrile; Sigma T182, 5 mg: FW = 282.38. Alternative source: Enzo, BML-EI215.
-:::: <span style="color:#8B008B"> '''Caution:''' Toxic!</span>
-::: '''Preparation of 50 mM stock solution''' (dissolved in 99.9% ethanol):
-::::# To 5 mg in a glass vial: dissolve with 354.1 µL ethanol.
-::::# Store in glass vials at -20 °C.
-::: '''Preparation of 500 µM stock solution''' (dissolved in 99.9% ethanol):
-::::# Add 990 µL ethanol and 10 µL of the 50 mM stock solution to a glass vial.
-::::# Store in glass vials at -20 °C.
-::: '''Preparation of 50 µM stock solution''' (dissolved in 99.9% ethanol):
-::::# Add 900 µL ethanol and 100 µL of the 500 µM stock solution to a glass vial.
-::::# Divide into 0.1 ml portions, or store in a glass vial and use subsamples during experiments to avoid contamination of the storage solution.
-::::# Store in glass vials at -20 °C.
-:::: '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
-::: '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
-::::* Titration volume: 1 µl steps using a 10 µl syringe (2 ml O2k-Chamber) with the 50 µM solution.
-::::* Final concentration: 25 nM steps.
-{{MitoPedia topics|mitopedia topic=Uncoupler}}