Ruthenium red: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr= | |abbr=RR | ||
|description='''Ruthenium red''' (synonym: ammoniated ruthenium oxychloride) inhibits the mitochondrial Ca<sup>2+</sup> uniporter. However, in addition it has been shown to interact with and inhibit a large number of other proteins, including ion channels particularly of the Transient Receptor Potential Vanilloid (TRPV) family [1], Ca<sup>2+</sup>-ATPases, and, importantly, the voltage-dependent anion channel (VDAC) [2]. | |description='''Ruthenium red''' (synonym: ammoniated ruthenium oxychloride) inhibits the mitochondrial Ca<sup>2+</sup> uniporter. However, in addition it has been shown to interact with and inhibit a large number of other proteins, including ion channels particularly of the Transient Receptor Potential Vanilloid (TRPV) family [1], Ca<sup>2+</sup>-ATPases, and, importantly, the voltage-dependent anion channel (VDAC) [2]. | ||
|info=[[MiPNet03.02]], [http://en.wikipedia.org/wiki/Ruthenium_red Wikipedia] | |info=[[MiPNet03.02]], [http://en.wikipedia.org/wiki/Ruthenium_red Wikipedia] | ||
}} | }} | ||
__TOC__ | |||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
{{Chemical_description | |||
|abbr=RR | |||
|trivial name=Ruthenium red | |||
|complete name=Ammoniated ruthenium oxychloride | |||
|chem formula=(NH<sub>3</sub>)<sub>5</sub>RuORu(NH<sub>3</sub>)<sub>4</sub>ORu(NH<sub>3</sub>)<sub>5</sub>Cl<sub>6</sub> | |||
|molar mass=786.35 | |||
|vendor=Sigma-Aldrich | |||
|product number=R2751 | |||
|store at=RT | |||
|sensitivity= | |||
|cas=11103-72-3 | |||
|h statements=H302 | |||
|h info=Harmful if swallowed | |||
}}<!--:::'''Rut: Ruthenium Red''' (technical grade; [(NH<sub>3</sub>)<sub>5</sub>RuORu(NH<sub>3</sub>)<sub>4</sub>ORu(NH<sub>3</sub>)<sub>5</sub>]Cl<sub>6</sub>);ย Sigma R 2751, 1 g; M = 786.35 gยทmol<sup>-1</sup>.--> | |||
::::'''Preparation of 2 mM stock solution''' (dissolved in H<sub>2</sub>O)ย for '''2-mL O2k-chamber''': | |||
::::# Weigh 1.57 mg Ruthenium Red. | |||
::::# Dissolve in 1 mL H<sub>2</sub>O. | |||
::::# Divide into 0.1 mL aliquots. | |||
::::# Store at -20 ยฐC. | |||
:::ยป '''O2k manual titrations'''ย [[MiPNet09.12 O2k-Titrations]] | |||
::::* Titration volume ('''2-mL O2k-chamber'''): 1 ยตL using a 10 ยตL Hamilton syringe. | |||
::::* Final concentration: 1 ยตM. | |||
:::: '''Preparation of 0.5 mM stock solution''' (dissolved in H<sub>2</sub>O) for '''0.5-mL O2k-chamber''': | |||
::::# Take 50 ยตL of the 1 mM stock solution. | |||
::::# Add 150 ยตL H<sub>2</sub>O. | |||
:: | :::ยป '''O2k manual titrations'''ย [[MiPNet09.12 O2k-Titrations]] | ||
:: | ย | ||
:: | ::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 ยตL using a 10 ยตL Hamilton syringe. | ||
:: | ::::* Final concentration: 1 ยตM. | ||
== References == | == References == | ||
Line 24: | Line 47: | ||
# Amann R, Maggi CA (1991) Ruthenium red as a capsaicin antagonist.Life Sci. 9: 849-856. | # Amann R, Maggi CA (1991) Ruthenium red as a capsaicin antagonist.Life Sci. 9: 849-856. | ||
# Israelson A, Zaid H, Abu-Hamad S, Nahon E, Shoshan-Barmatz V (2008) Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1. Cell Calcium 43:196-204. | # Israelson A, Zaid H, Abu-Hamad S, Nahon E, Shoshan-Barmatz V (2008) Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1. Cell Calcium 43:196-204. | ||
{{MitoPedia methods}} | |||
{{MitoPedia topics | |||
|mitopedia topic=Inhibitor | |||
}} |
Latest revision as of 12:07, 15 June 2021
Description
Ruthenium red (synonym: ammoniated ruthenium oxychloride) inhibits the mitochondrial Ca2+ uniporter. However, in addition it has been shown to interact with and inhibit a large number of other proteins, including ion channels particularly of the Transient Receptor Potential Vanilloid (TRPV) family [1], Ca2+-ATPases, and, importantly, the voltage-dependent anion channel (VDAC) [2].
Abbreviation: RR
Reference: MiPNet03.02, Wikipedia
Application in HRR
- RR: Ruthenium red (Ammoniated ruthenium oxychloride; (NH3)5RuORu(NH3)4ORu(NH3)5Cl6), Sigma-Aldrich: R2751, store at RT, CAS: 11103-72-3, M = 786.35 gยทmol-1
- Hazard statements: H302; Harmful if swallowed
- RR: Ruthenium red (Ammoniated ruthenium oxychloride; (NH3)5RuORu(NH3)4ORu(NH3)5Cl6), Sigma-Aldrich: R2751, store at RT, CAS: 11103-72-3, M = 786.35 gยทmol-1
- Preparation of 2 mM stock solution (dissolved in H2O) for 2-mL O2k-chamber:
- Weigh 1.57 mg Ruthenium Red.
- Dissolve in 1 mL H2O.
- Divide into 0.1 mL aliquots.
- Store at -20 ยฐC.
- ยป O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 1 ยตL using a 10 ยตL Hamilton syringe.
- Final concentration: 1 ยตM.
- Preparation of 0.5 mM stock solution (dissolved in H2O) for 0.5-mL O2k-chamber:
- Take 50 ยตL of the 1 mM stock solution.
- Add 150 ยตL H2O.
- ยป O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (0.5-mL O2k-chamber): 1 ยตL using a 10 ยตL Hamilton syringe.
- Final concentration: 1 ยตM.
References
- Amann R, Maggi CA (1991) Ruthenium red as a capsaicin antagonist.Life Sci. 9: 849-856.
- Israelson A, Zaid H, Abu-Hamad S, Nahon E, Shoshan-Barmatz V (2008) Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1. Cell Calcium 43:196-204.
MitoPedia topics:
Inhibitor