Ruthenium red: Difference between revisions
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|info=[[MiPNet03.02]], [http://en.wikipedia.org/wiki/Ruthenium_red Wikipedia] | |info=[[MiPNet03.02]], [http://en.wikipedia.org/wiki/Ruthenium_red Wikipedia] | ||
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''Rut: Ruthenium Red''' (technical grade; [(NH<sub>3</sub>)<sub>5</sub>RuORu(NH<sub>3</sub>)<sub>4</sub>ORu(NH<sub>3</sub>)<sub>5</sub>]Cl<sub>6</sub>); Sigma R 2751, 1 g; FW = 786.35. | :::'''Rut: Ruthenium Red''' (technical grade; [(NH<sub>3</sub>)<sub>5</sub>RuORu(NH<sub>3</sub>)<sub>4</sub>ORu(NH<sub>3</sub>)<sub>5</sub>]Cl<sub>6</sub>); Sigma R 2751, 1 g; FW = 786.35. | ||
::::'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O): | |||
::::# Weigh 7.86 mg Ruthenium Red. | |||
::::# Dissolve in 1 mL H<sub>2</sub>O. | |||
:: | ::::# Divide into 0.2 mL aliquots. | ||
:: | ::::# Store at -20 °C. | ||
:: | |||
:: | |||
== References == | == References == | ||
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# Amann R, Maggi CA (1991) Ruthenium red as a capsaicin antagonist.Life Sci. 9: 849-856. | # Amann R, Maggi CA (1991) Ruthenium red as a capsaicin antagonist.Life Sci. 9: 849-856. | ||
# Israelson A, Zaid H, Abu-Hamad S, Nahon E, Shoshan-Barmatz V (2008) Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1. Cell Calcium 43:196-204. | # Israelson A, Zaid H, Abu-Hamad S, Nahon E, Shoshan-Barmatz V (2008) Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1. Cell Calcium 43:196-204. | ||
{{MitoPedia methods}} | |||
{{MitoPedia topics | |||
|mitopedia topic=Inhibitor | |||
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Revision as of 10:33, 11 September 2020
Description
Ruthenium red (synonym: ammoniated ruthenium oxychloride) inhibits the mitochondrial Ca2+ uniporter. However, in addition it has been shown to interact with and inhibit a large number of other proteins, including ion channels particularly of the Transient Receptor Potential Vanilloid (TRPV) family [1], Ca2+-ATPases, and, importantly, the voltage-dependent anion channel (VDAC) [2].
Abbreviation: Rut
Reference: MiPNet03.02, Wikipedia
Application in HRR
- Rut: Ruthenium Red (technical grade; [(NH3)5RuORu(NH3)4ORu(NH3)5]Cl6); Sigma R 2751, 1 g; FW = 786.35.
- Preparation of 10 mM stock solution (dissolved in H2O):
- Weigh 7.86 mg Ruthenium Red.
- Dissolve in 1 mL H2O.
- Divide into 0.2 mL aliquots.
- Store at -20 °C.
References
- Amann R, Maggi CA (1991) Ruthenium red as a capsaicin antagonist.Life Sci. 9: 849-856.
- Israelson A, Zaid H, Abu-Hamad S, Nahon E, Shoshan-Barmatz V (2008) Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1. Cell Calcium 43:196-204.
MitoPedia topics:
Inhibitor