Rotenone: Difference between revisions
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|abbr=Rot | |abbr=Rot | ||
|description=(2R,6aS,12aS)-1,2,6,6a,12,12a-hexahydro-2-isopropenyl-8,9 dimethoxychromeno[3,4-b]furo(2,3-h)chromen-6-on; C<sub>23</sub>H<sub>22</sub>O<sub>6</sub>. Inhibitor of [[complex I]] via inhibition of NADH oxidation.It inhibits the transfer of electrons from iron-sulfur centers in CI to ubiquinone via binding to the ubiquinone binding site of CI. See also [[S(Rot)|S<sub>(Rot)</sub>]]. | |description=(2R,6aS,12aS)-1,2,6,6a,12,12a-hexahydro-2-isopropenyl-8,9 dimethoxychromeno[3,4-b]furo(2,3-h)chromen-6-on; C<sub>23</sub>H<sub>22</sub>O<sub>6</sub>. Inhibitor of [[complex I]] via inhibition of NADH oxidation.It inhibits the transfer of electrons from iron-sulfur centers in CI to ubiquinone via binding to the ubiquinone binding site of CI. See also [[S(Rot)|S<sub>(Rot)</sub>]]. | ||
|info=MiPNet11.09, MiPNet09.12, MiPNet12.15, MiPNet03.02 | |info=[[MiPNet11.09]], [[MiPNet09.12]], [[MiPNet12.15]], [[MiPNet03.02]] | ||
|type=Inhibitor ETS | |type=Inhibitor ETS | ||
}} | }} | ||
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'''Preparation of 0.1 mM stock solution''' <ref>Note: Up to 2006, rotentone was added at a higher final concentration 0.5 µM), hence a 0.5 mM stock solution was prepared. Since 0.05 µM may be fully inhibiting, we suggest to use a stock solution at lower concentration (0.1 mM), to reduce the problem or rotenone retention.</ref> (dissolved in 99.9% Ethanol): | '''Preparation of 0.1 mM stock solution''' <ref>Note: Up to 2006, rotentone was added at a higher final concentration 0.5 µM), hence a 0.5 mM stock solution was prepared. Since 0.05 µM may be fully inhibiting, we suggest to use a stock solution at lower concentration (0.1 mM), to reduce the problem or rotenone retention.</ref> (dissolved in 99.9% Ethanol): | ||
::1) weigh 0.39 mg of Rotenone | ::1) weigh 0.39 mg of Rotenone and dissolve in 10 ml of absolute ethanol | ||
::2) | ::2) divide into small portions (use dark glass vials) | ||
::3) store frozen at -20 °C | ::3) store frozen at -20 °C | ||
Revision as of 13:10, 3 December 2010
- high-resolution terminology - matching measurements at high-resolution
Rotenone
Description
(2R,6aS,12aS)-1,2,6,6a,12,12a-hexahydro-2-isopropenyl-8,9 dimethoxychromeno[3,4-b]furo(2,3-h)chromen-6-on; C23H22O6. Inhibitor of complex I via inhibition of NADH oxidation.It inhibits the transfer of electrons from iron-sulfur centers in CI to ubiquinone via binding to the ubiquinone binding site of CI. See also S(Rot).
Abbreviation: Rot
Reference: MiPNet11.09, MiPNet09.12, MiPNet12.15, MiPNet03.02
Labels:
Enzyme: Complex I
HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Application in HRR
Rot: Rotenone (Rotenone ≥ 95%, C23H22O6); Sigma R 8875, 1 g, store at R.T.: FW = 394.42)
Caution: Very toxic and light sensitive (store solution in a dark glass vial).
Preparation of 0.1 mM stock solution [1] (dissolved in 99.9% Ethanol):
- 1) weigh 0.39 mg of Rotenone and dissolve in 10 ml of absolute ethanol
- 2) divide into small portions (use dark glass vials)
- 3) store frozen at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe
- Final conc. in 2 ml O2k-chamber: 0.5 µM
- ↑ Note: Up to 2006, rotentone was added at a higher final concentration 0.5 µM), hence a 0.5 mM stock solution was prepared. Since 0.05 µM may be fully inhibiting, we suggest to use a stock solution at lower concentration (0.1 mM), to reduce the problem or rotenone retention.
