Difference between revisions of "Oroboros FAQ"

Latest revision as of 11:36, 8 November 2023

Oroboros FAQ

Applications and samples

Is the O2k easy to use in standard applications of mitochondrial respiration?

The O2k is a robust instrument, ready to be used for standard applications, providing high reliability, stability of the oxygen sensor over several months, and full software support. See: DatLab

Most 'oxygraphs' are designed for analyzing isolated mitochondria. Which types of samples can be analyzed with the O2k?

The O2k can be routinely applied for cultured cells, living cells and tissue biopsies, permeabilized cells and tissue biopsies, microbial cultures, oxygen-consuming enzymes, antioxidant systems, and, of course, isolated mitochondria. See: Respirometry and O2k-Publications.

Can the O2k be applied for the measurement of oxygen consumption in delicate mammalian cells, when limited numbers of cells are available?

Compared to other instruments, the O2k requires five to ten times lower cell numbers for routine measurements of cell respiration, making it highly cost-, personnel- and time-efficient.

Can the O2k be applied for measuring human biopsies?

When permeabilized biopsy preparations are utilized, the O2k provides the unique potential to reduce sample size to as little as 1 mg fresh weight per experimental run, supporting application of complex titration protocols.

Can the O2k be applied to measure tissue oxygen concentration?

No. The O2k is a 'chamber' approach, for application with tissue biopsies, cultured cells or isolated mitochondria. Whereas HRR cannot be applied for measurement of tissue O₂ concentration, it is the method of choice for analysis of tissue O₂ consumption and functional diagnosis of mitochondrial injuries.

Can the O2k be applied for measurement of oxygen consumption in adherent cells?

Attached cells may be grown on disks before being introduced into the cylindrical chamber of the O2k. Alternatively, cells attached to beads can be used. The Sample Holder may be useful for these applications. Alternatively, gentle stirring, with the use of specific small stirring bars, is advisable to avoid crashing of the beads. Routinely, we apply the O2k on adherent cells after transfering them into suspension. For small cells, such as fibroblasts, at least 200,000 cells per chamber are required. More information: Zdrazilova 2022 PLOS ONE

How many parameters can be measured simultaneously?

The O2k is designed to measure respiration plus one additional parameter at a time. It might be possible to measure more than one additional parameter at a time with the O2k-Q-Module and O2k-Fluo Smart-Module.

Delivery content, warranty and support

Does the purchase include installation, setup and training?

Installation and setup

On-site installation and setup is not included in the purchase of an Oroboros O2k. Setup and installation are usually performed by the customers themselves with the help of our detailed and user-friendly O2k-Manual which is sent out with the O2k. In addition, the manual can also be easily down-loaded form our website (O2k-Manual). There is also a section for technical service. Our technical specialists, based here in Innsbruck, can also assist in helping to locate and solve any technical problems, should these arise. Based on our experience and feedback received from customers all over the world, we are confident that you will easily master the set-up and installation.

Training

The purchase of an O2k includes a waived registration fee for our O2k-Workshop, which takes place at regular intervals all over the world. For more information on our upcoming O2k-Workshops (IOC), please take a look at Oroboros Events or send an Email to: [email protected].

Is it possible to extend the warranty period?

Yes, it is possible to extend the warranty period by a total of three years. For more details, please contact our office: [email protected]

Do you offer the option to lease or rent your instruments?

It is possible to lease the O2k over the course of three years, after which the instrument passes over into your ownership. Furthermore, you can rent an instrument for a short period (e.g. several weeks or months) if you need to improve your throughput for a specific study or experiment. Contact our office for further details: [email protected]

Are you registered with System for Award Management (SAM)?

Yes.

@@ Line 1: / Line 1: @@
-== O2k FAQ ==
+{{OROBOROS header page name}}
-'''IS THE OROBOROS OXYGRAPH-2K (O2K) EASY TO USE IN STANDARD APPLICATIONS OF MITOCHONDRIAL RESPIRATION?'''
-Yes! The O2k is a robust instrument, ready to be used for standard applications, providing high reliability, stability of the oxygen sensor over several months, and full software support. See: [[DatLab]]
+__TOC__
+== Applications and samples ==
-'''MOST 'OXYGRAPHS' ARE DESIGNED TO ANALYZE ISOLATED MOTOCHONDRIA. WHICH TYPES OF SAMPLES CAN BE ANALYZED WITH THE O2K?'''
-The O2k can be routinely applied for cultured cells, intact cells and tissue biopsies, permeabilized cells and tissue biopsies, microbial cultures, oxygen-consuming enzymes, antioxidant systems, and, of course, isolated mitochondria. See: [[Respirometry]]
+<div class="toccolours mw-collapsible mw-collapsed" style="background:#F7F8FF">
+<span style="font-size:105%; color:#424242">'''Is the O2k easy to use in standard applications of mitochondrial respiration?'''</span>
+<div class="mw-collapsible-content"> The O2k is a robust instrument, ready to be used for standard applications, providing high reliability, stability of the oxygen sensor over several months, and full software support. See: [[DatLab|DatLab]]</div>
+</div>
+<div class="toccolours mw-collapsible mw-collapsed">
+<span style="font-size:105%; color:#424242"> '''Most 'oxygraphs' are designed for analyzing isolated mitochondria. Which types of samples can be analyzed with the O2k?'''</span>
+<div class="mw-collapsible-content"> The O2k can be routinely applied for cultured cells, [[living cells]] and tissue biopsies, [[Permeabilized tissue or cells|permeabilized cells]] and tissue biopsies, microbial cultures, oxygen-consuming enzymes, antioxidant systems, and, of course, [[Isolated mitochondria|isolated mitochondria]]. See: [[Respirometry|Respirometry]] and [[O2k-Publications: Topics|O2k-Publications]].</div>
+</div>
-'''WHAT IS SPECIAL ABOUT HIGH_RESOLUTION RESPIROMETRY?'''
+<div class="toccolours mw-collapsible mw-collapsed" style="background:#F7F8FF">
+<span style="font-size:105%; color:#424242"> '''Can the O2k be applied for the measurement of oxygen consumption in delicate mammalian cells, when limited numbers of cells are available?'''</span>
+<div class="mw-collapsible-content"> Compared to other instruments, the O2k requires five to ten times lower cell numbers for routine measurements of cell respiration, making it highly cost-, personnel- and time-efficient.</div>
+</div>
-The O2k is a sole source apparatus, with no other available instrument meeting its specifications for high-resolution respirometry.
+<div class="toccolours mw-collapsible mw-collapsed">
+<span style="font-size:105%; color:#424242">'''Can the O2k be applied for measuring human biopsies?'''</span>
+<div class="mw-collapsible-content"> When [[Permeabilized tissue or cells|permeabilized]] biopsy preparations are utilized, the O2k provides the unique potential to reduce sample size to as little as 1 mg fresh weight per experimental run, supporting application of complex titration protocols.</div>
+</div>
-O2k high-resolution respirometry (HRR) is based on the OROBOROS O2k, combining chamber design, application of oxygen-tight materials, electrochemical and optical sensors, Peltier-temperature control and software features (DatLab) to obtain the unique sensitive and quantitative resolution of oxygen concentration and oxygen flux, with a closed-chamber or open-chamber mode of operation (TIP2k). See: [[High-Resolution_Respirometry]]
+<div class="toccolours mw-collapsible mw-collapsed" style="background:#F7F8FF">
+<span style="font-size:105%; color:#424242">'''Can the O2k be applied to measure tissue oxygen concentration?'''</span>
+<div class="mw-collapsible-content"> No. The O2k is a [[O2k-chamber|'chamber']] approach, for application with tissue biopsies, cultured cells or [[Isolated_mitochondria|isolated mitochondria]]. Whereas HRR cannot be applied for measurement of tissue O<sub>2</sub> concentration, it is the method of choice for analysis of tissue O<sub>2</sub> consumption and functional diagnosis of mitochondrial injuries.</div>
+</div>
+<div class="toccolours mw-collapsible mw-collapsed">
+<span style="font-size:105%; color:#424242">'''Can the O2k be applied for measurement of oxygen consumption in [[living cells|adherent cells]]?'''</span>
+<div class="mw-collapsible-content"> Attached cells may be grown on disks before being introduced into the [[O2k-chamber|cylindrical chamber]] of the O2k. Alternatively, cells attached to beads can be used. The [[Sample Holder]] may be useful for these applications. Alternatively, gentle stirring, with the use of specific small [[Stirrer-Bar\white PVDF\15x6 mm|stirring bars]], is advisable to avoid crashing of the beads. Routinely, we apply the O2k on adherent cells after transfering them into suspension. For small cells, such as fibroblasts, at least 200,000 cells per chamber are required.
+More information: [[Zdrazilova 2022 PLOS ONE]]</div>
+</div>
-'''WHAT ARE THE MOST COMMON O2K APPLICATIONS?'''
+<div class="toccolours mw-collapsible mw-collapsed" style="background:#F7F8FF">
+<span style="font-size:105%; color:#424242">'''How many parameters can be measured simultaneously?'''</span>
+<div class="mw-collapsible-content"> The O2k is designed to measure respiration plus one additional parameter at a time. It might be possible to measure more than one additional parameter at a time with the O2k-Q-Module and O2k-Fluo Smart-Module.
+</div>
+</div>
-The most common applications for the OROBOROS O2k are the following:
-* Diagnosis of acquired and genetic mitochondrial diseases
-* Diagnosis of myopathies and neuromuscular pathologies
-* Studies on cell function and cell death - apoptosis and necrosis
-* Oxidative stress
-* Ischemia-reperfusion injury
-* Aging and senescence
-* Pharmacological tests
-For more applications, browse through the [[O2k-Publications]].
+== Delivery content, warranty and support ==
-'''Can the O2k be applied for the measurement of oxygen consumption in delicate mammalian cells, when limited numbers of cells are available?'''
+<div class="toccolours mw-collapsible mw-collapsed">
+<span style="font-size:105%; color:#424242">'''Does the purchase include installation, setup and training?'''</span>
+<div class="mw-collapsible-content">
+* '''Installation and setup'''
+On-site installation and setup is not included in the purchase of an [[Oroboros O2k]]. Setup and installation are usually performed by the customers themselves with the help of our detailed and user-friendly [[O2k-Manual]] which is sent out with the O2k. In addition, the manual can also be easily down-loaded form our website ([[O2k-Manual]]). There is also a section for [[O2k-technical_service|technical service]]. Our technical specialists, based here in Innsbruck, can also assist in helping to locate and solve any technical problems, should these arise. Based on our experience and feedback received from customers all over the world, we are confident that you will easily master the set-up and installation.
+<br/>
+* '''Training'''
+The purchase of an O2k includes a waived registration fee for our '''[[O2k-Workshop]]''', which takes place at regular intervals all over the world. For more information on our upcoming O2k-Workshops (IOC), please take a look at '''[[Oroboros Events]]''' or send an Email to: [mailto:[email protected] [email protected]].</div>
+</div>
-With the OROBOROS Oxygraph-2k, five to ten times lower cell numbers are required for routine measurements of cell respiration, compared to other instruments. It is important to evaluate cost-effective high output per unit of resources (financial, personnel, time).
+<div class="toccolours mw-collapsible mw-collapsed">
+<span style="font-size:105%; color:#424242">'''Is it possible to extend the warranty period?'''</span>
+<div class="mw-collapsible-content">
+Yes, it is possible to extend the warranty period by a total of three years. For more details, please contact our office: [mailto:instruments@oroboros.at instruments@oroboros.at]
+</div>
+</div>
+<div class="toccolours mw-collapsible mw-collapsed">
+<span style="font-size:105%; color:#424242">'''Do you offer the option to lease or rent your instruments?'''</span>
+<div class="mw-collapsible-content">
+It is possible to lease the O2k over the course of three years, after which the instrument passes over into your ownership. Furthermore, you can rent an instrument for a short period (e.g. several weeks or months) if you need to improve your throughput for a specific study or experiment. Contact our office for further details: [mailto:[email protected] [email protected]]
+</div>
+</div>
-'''Can the O2k be applied to measure human biopsies?'''
+<div class="toccolours mw-collapsible mw-collapsed" style="background:#F7F8FF">
+<span style="font-size:105%; color:#424242">'''Are you registered with System for Award Management (SAM)?'''</span>
-With permeabilized biopsy preparations, the O2k provides the unique potential to reduce the amount of biopsy to as little as 1 mg fresh weight per experimental run, with application of complex titration protocols.
+<div class="mw-collapsible-content">
+Yes.
+</div>
-'''Can the O2k be applied to measure tissue oxygen concentration?'''
+</div>
-The O2k is a 'chamber' approach, for application with tissue biopsies, cultured cells or isolated mitochondria. Whereas HRR cannot be applied for measurement of tissue O2 concentration, it is the method of choice for analysis of tissue O2 consumption and functional diagnosis of mitochondrial injuries.
-'''Can the Oxygraph-2k be applied for the measurement of oxygen consumption in adherent cells?'''
-Attached cells may be introduced into the chamber by growing them on a disk, which then is inserted into the cylindrical chamber. Alternatively, cells attached to beads may be used, but only with gentle stirring which does not crash the beads, which requires application of a specific small stirrer flee. Routinely, we apply the Oxygraph-2k on adherent cells after bringing the cells into suspension. For small cells such as fibroblasts, we need 200,000 cells per chamber.