O2k-Open Support: Difference between revisions
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=== O2k Quality Control === | [[File:MitoFit-QCS.jpg|right|400px|MitoFit-QCS]] | ||
== O2k-Open Support: service instructions == | |||
::::* '''O2k-Open Support''' includes Open Access pages that help to optimally use the O2k, to apply instrumental O2k-Quality Control, and perform user-service when needed. | |||
::::::# [[MitoPedia: O2k]]: find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages, specifically on the O2k and high-resolution respirometry | |||
::::::# | |||
::::::# | |||
::::::# | |||
== O2k-Quality Control == | |||
:::: Many commonly raised issues of O2k-technical support are solved in the newest version of [[DatLab]]. | :::: Many commonly raised issues of O2k-technical support are solved in the newest version of [[DatLab]]. | ||
:::: The '''O2 sensor and O2k chamber tests''' are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of [[solution protocols]] (preparation of chemicals), [[sample]] preparation protocols (such as [[mitochondrial preparations]]), and [[SUIT protocols]]. | |||
::::# Clean O2k-chambers and contamination-free incubation medium are required. | |||
::::# Analysis of DatLab files recorded in instrumental QC tests is essential. | |||
:::: The '''O2 sensor and O2k chamber tests''' are important to eliminate instrumental artefacts ( | |||
::::# Biological experiments are not suitable for trouble shooting. | ::::# Biological experiments are not suitable for trouble shooting. | ||
:::: The '''sequence''' of | :::: The '''sequence''' of instrumental QC tests is strictly | ||
::::# O2 sensor test | ::::# O2 sensor test | ||
::::# O2k-chamber test | ::::# O2k-chamber test | ||
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[[File:O2 sensor test.jpg|right|400px|O2 sensor test|link=MiPNet06.03 POS-calibration-SOP]] | [[File:O2 sensor test.jpg|right|400px|O2 sensor test|link=MiPNet06.03 POS-calibration-SOP]] | ||
=== O2 sensor test === | |||
:::: | :::: The [[OroboPOS|OroboPOS]] (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date: | ||
::::» O2 sensor test: [[Oxygen_sensor_test|'''Oxygen sensor test''']] | ::::» O2 sensor test: [[Oxygen_sensor_test |'''QC1: Oxygen sensor test''']] | ||
=== O2k-chamber test === | |||
:::: If all quality control criteria of the O2 sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail: | |||
::::» O2k-chamber test: [[MiPNet14.06_Instrumental_O2_background |'''QC2: Instrumental O<sub>2</sub> background''']] | |||
=== Search for defective components: the 2-chamber design of the O2k === | |||
:::: The '''two chamber design''' of the O'''2k''' has many benefits, including advantages in '''trouble shooting'''. By switching components between sides A and B of the O2k, it is usually possible to locate an instrumental problem, finding selectively a specific defective component of the O2k. This component can then be serviced by the end user or replaced by Oroboros without the need to ship the entire O2k. | |||
== O2k-Open Support by Oroboros experts == | |||
::::* O2k-Open Support is given to individual users by Oroboros experts through our [[O2k-ticket system]]. | |||
::::* We welcome and value feedback. Technical and scientific questions, which are not yet addressed in the available guidelines or are difficult to trace on our website, are relevant to many specialists and beginners in the field. | |||
::::* O2k-Open Support aims at sharing our support communication openly with the scientific community, (''1'') improving the scope and documentation of the O2k-technology, and (''2'') providing [[Open Access]] educational examples on answering questions and finding solutions - in the spirit of [[Gentle_Science|Gentle Science]]. | |||
::::* O2k-Open Support by Oroboros experts does not replace the obligation to consult the [[O2k-Manual|O2k-Manual]] and relevant chapters on [[O2k-Procedures]] prior to contacting the O2k-support team. | |||
=== | === O2k-Open Support agreement === | ||
::::* An O2k-Open Support agreement is required for each individual case. The relevant correspondence or a summary of the correspondence may then be published on our website. Our users can decide if their names, institutional and Email addresses are included. | |||
::::* Regular consultancy fees apply for O2k-Support requested without O2k-Open Support agreement. | |||
=== Repair === | |||
:::: Hardware problems may require replacement of a defective spare part. Some electronic or mechanical defects may be solved only by repair of the O2k in the electronics workshop of Oroboros Instruments, ''e.g.'', a defective Peltier unit. | |||
:::: A cost estimation for repair of the O2k-Main Unit due to an electronic or mechanical defect can only be given after inspection of the O2k | :::: A cost estimation for repair of the O2k-Main Unit due to an electronic or mechanical defect can only be given after inspection of the O2k in our electronics workshop in Austria. Once a cost estimation has been provided, the repair is carried out with the customer’s consent. For shipment of the O2k, follow the [[Shipping_an_oxygraph|detailed instructions]]. | ||
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:::: '''»''' [[O2k-Network_discussion_forum|O2k-Network discussion forum]] | :::: '''»''' [[O2k-Network_discussion_forum|O2k-Network discussion forum]] | ||
== Contact == | == Contact == |
Revision as of 14:02, 29 May 2019
O2k-Open Support |
- we provide service and support based on open innovation
O2k-Open Support: service instructions
- O2k-Open Support includes Open Access pages that help to optimally use the O2k, to apply instrumental O2k-Quality Control, and perform user-service when needed.
- MitoPedia: O2k: find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages, specifically on the O2k and high-resolution respirometry
O2k-Quality Control
- Many commonly raised issues of O2k-technical support are solved in the newest version of DatLab.
- The O2 sensor and O2k chamber tests are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of solution protocols (preparation of chemicals), sample preparation protocols (such as mitochondrial preparations), and SUIT protocols.
- Clean O2k-chambers and contamination-free incubation medium are required.
- Analysis of DatLab files recorded in instrumental QC tests is essential.
- Biological experiments are not suitable for trouble shooting.
- The O2 sensor and O2k chamber tests are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of solution protocols (preparation of chemicals), sample preparation protocols (such as mitochondrial preparations), and SUIT protocols.
- The sequence of instrumental QC tests is strictly
- O2 sensor test
- O2k-chamber test
- The sequence of instrumental QC tests is strictly
O2 sensor test
- The OroboPOS (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:
- » O2 sensor test: QC1: Oxygen sensor test
O2k-chamber test
- If all quality control criteria of the O2 sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:
- » O2k-chamber test: QC2: Instrumental O2 background
Search for defective components: the 2-chamber design of the O2k
- The two chamber design of the O2k has many benefits, including advantages in trouble shooting. By switching components between sides A and B of the O2k, it is usually possible to locate an instrumental problem, finding selectively a specific defective component of the O2k. This component can then be serviced by the end user or replaced by Oroboros without the need to ship the entire O2k.
O2k-Open Support by Oroboros experts
- O2k-Open Support is given to individual users by Oroboros experts through our O2k-ticket system.
- We welcome and value feedback. Technical and scientific questions, which are not yet addressed in the available guidelines or are difficult to trace on our website, are relevant to many specialists and beginners in the field.
- O2k-Open Support aims at sharing our support communication openly with the scientific community, (1) improving the scope and documentation of the O2k-technology, and (2) providing Open Access educational examples on answering questions and finding solutions - in the spirit of Gentle Science.
- O2k-Open Support by Oroboros experts does not replace the obligation to consult the O2k-Manual and relevant chapters on O2k-Procedures prior to contacting the O2k-support team.
O2k-Open Support agreement
- An O2k-Open Support agreement is required for each individual case. The relevant correspondence or a summary of the correspondence may then be published on our website. Our users can decide if their names, institutional and Email addresses are included.
- Regular consultancy fees apply for O2k-Support requested without O2k-Open Support agreement.
Repair
- Hardware problems may require replacement of a defective spare part. Some electronic or mechanical defects may be solved only by repair of the O2k in the electronics workshop of Oroboros Instruments, e.g., a defective Peltier unit.
- A cost estimation for repair of the O2k-Main Unit due to an electronic or mechanical defect can only be given after inspection of the O2k in our electronics workshop in Austria. Once a cost estimation has been provided, the repair is carried out with the customer’s consent. For shipment of the O2k, follow the detailed instructions.
The O2k-Network
- The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.
- » The O2k-Network Labs: » O2k-Network
Contact
- » In case you need further technical support and for your valuable feedback, please contact [email protected]
- » Oroboros MitoFit Laboratory - open for innovation and cooperation.
- » Oroboros Science Scholarships - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry