NADH electron transfer-pathway state: Difference between revisions
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|abbr=N | |abbr=N | ||
|description=[[File:SUIT-catg N.jpg|right|300px|N-junction]] | |description=[[File:SUIT-catg N.jpg|right|300px|N-junction]] | ||
[[NADH]]-linked substrates (CI-linked) are [[ETS substrate types | | [[NADH]]-linked substrates (CI-linked) are [[ETS substrate types |N-junction substrates]] of ETS-level 4, feeding electrons into the [[N-junction]] catalyzed by various mt-dehydrogenases. N-supported flux is induced in mt-preparations by addition of NADH-generating substrate combinations of [[pyruvate]] (P), [[glutamate]] (G), [[malate]] (M), [[oxaloacetate]] (Oa), [[oxoglutarate]] (Og), [[citrate]], [[hydroxybutyrate]]. These N-junction substrates are (indirectly) linked to [[Complex I]] by the corresponding dehydrogenase-catalyzed reactions reducing NAD<sup>+</sup> to NADH+H<sup>+</sup>. The most commonly applied N-junction substrate combinations are: [[PM]], [[GM]], [[PGM]]. [[Malate alone]] is a special case related to [[malic enzyme]] (mtME). [[Glutamate alone]] supports respiration through [[glutamate dehydrogenase]] (mtGDH). In mt-preparations, succinate dehydrogenase (SDH; [[CII]]) is largely substrate-limited in N-linked respiration, due to metabolite depletion into the incubation medium. The residual involvement of S-linked respiration in the pesence of N-junction substrates can be further suppressed by the CII-inhibitor [[malonic acid]]). | ||
|info=[[Gnaiger 2014 MitoPathways]] | |info=[[Gnaiger 2014 MitoPathways]] | ||
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|mitopedia method=Respirometry | |mitopedia method=Respirometry | ||
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Contributed by [[Gnaiger E]] 2016-03-20; edited 2016-08-26, 2016-09- | Contributed by [[Gnaiger E]] 2016-03-20; edited 2016-08-26, 2016-09-10. | ||
= List of publications: N-linked or CI-linked substrate state = | = List of publications: N-linked or CI-linked substrate state = | ||
{{#ask:[[Category:Publications]] [[Substrate states::CI]] | {{#ask:[[Category:Publications]] [[Substrate states::CI]] |
Revision as of 07:50, 10 September 2016
- high-resolution terminology - matching measurements at high-resolution
NADH electron transfer-pathway state
Description
NADH-linked substrates (CI-linked) are N-junction substrates of ETS-level 4, feeding electrons into the N-junction catalyzed by various mt-dehydrogenases. N-supported flux is induced in mt-preparations by addition of NADH-generating substrate combinations of pyruvate (P), glutamate (G), malate (M), oxaloacetate (Oa), oxoglutarate (Og), citrate, hydroxybutyrate. These N-junction substrates are (indirectly) linked to Complex I by the corresponding dehydrogenase-catalyzed reactions reducing NAD+ to NADH+H+. The most commonly applied N-junction substrate combinations are: PM, GM, PGM. Malate alone is a special case related to malic enzyme (mtME). Glutamate alone supports respiration through glutamate dehydrogenase (mtGDH). In mt-preparations, succinate dehydrogenase (SDH; CII) is largely substrate-limited in N-linked respiration, due to metabolite depletion into the incubation medium. The residual involvement of S-linked respiration in the pesence of N-junction substrates can be further suppressed by the CII-inhibitor malonic acid).
Abbreviation: N
Reference: Gnaiger 2014 MitoPathways
MitoPedia concepts:
MiP concept,
Respiratory state,
SUIT concept,
SUIT state
MitoPedia methods:
Respirometry
Contributed by Gnaiger E 2016-03-20; edited 2016-08-26, 2016-09-10.