Myxothiazol: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=Myx | |abbr=Myx | ||
|description='''Myxothiazol''' | |description='''Myxothiazol''' Myx is an inhibitor of [[Complex III]] (CIII). CIII also inhibits [[Complex I|CI]] (G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome ''b''<sub>L</sub>) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein. | ||
|info=[[MiPNet09.12]] | |info=[[MiPNet09.12]] | ||
}} | }} |
Revision as of 13:17, 19 October 2021
Description
Myxothiazol Myx is an inhibitor of Complex III (CIII). CIII also inhibits CI (G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome bL) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein.
Abbreviation: Myx
Reference: MiPNet09.12
Application in HRR
- Myx: Myxothiazol (from Myxobacterium myxococcus fulvus; C25H33N3O3S2), Sigma-Aldrich: T5580, store at 4 ยฐC, CAS: 76706-55-3, M = 487.7 gยทmol-1
- Hazard statements: H300; fatal if swallowed
- Myx: Myxothiazol (from Myxobacterium myxococcus fulvus; C25H33N3O3S2), Sigma-Aldrich: T5580, store at 4 ยฐC, CAS: 76706-55-3, M = 487.7 gยทmol-1
- Available again from Sigma with this new product number. The old product number was M 5779.
- Preparation of 1 mM storage and stock solution (dissolved in ethanol abs.) for 2-mL O2k-chamber:
- Weigh 1 mg of myxothiazol into a small glass vial.
- Add 2.05 mL ethanol.
- Divide into 0.2 mL (use glass vials).
- Store at -20 ยฐC.
- Preparation of 1 mM storage and stock solution (dissolved in ethanol abs.) for 2-mL O2k-chamber:
- ยปO2k manual titrations MiPNet09.12
- Titration volume (2-mL O2k-chamber): 1 ยตL using a 10 ยตL Hamilton syringe.
- Final concentration: 0.5 ยตM.
- ยปO2k manual titrations MiPNet09.12
- Preparation of 0.25mM stock solution (dissolved in ethanol abs.) for 0.5-mL O2k-chamber:
- Take 100 ยตL of the 1 mM stock solution.
- Add 300 ยตL ethanol.
- Preparation of 0.25mM stock solution (dissolved in ethanol abs.) for 0.5-mL O2k-chamber:
- ยปO2k manual titrations MiPNet09.12
- Titration volume (0.5-mL O2k-chamber): 1 ยตL using a 10 ยตL Hamilton syringe.
- Final concentration: 0.5 ยตM.
- ยปO2k manual titrations MiPNet09.12
Compare
- Antimycin A is less expensive than Myx but induces higher ROS production.
MitoPedia topics:
Inhibitor