Myxothiazol: Difference between revisions
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::::<span style="color:#8B008B"> '''Caution:''' Very toxic.</span> | ::::<span style="color:#8B008B"> '''Caution:''' Very toxic.</span> | ||
::::'''Preparation of 1 mM storage and stock solution''' (dissolved in 99.9% ethanol) for '''2-mL O2k-chamber''': | ::::'''Preparation of 1 mM storage and stock solution''' (dissolved in 99.9% ethanol) for '''2-mL O2k-chamber''': |
Revision as of 12:01, 28 October 2020
Description
Myxothiazol, Myx, is an inhibitor of Complex III (CIII). CIII also inhibits CI (G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome bL) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein.
Abbreviation: Myx
Reference: MiPNet09.12
Application in HRR
- Myx: Myxothiazol (from Myxobacterium myxococcus fulvus, ~95%, HPLC), C25H33N3O3S2); Sigma T 5580, 1 mg, store at 4 ยฐC: FW = 487.7. Available again from Sigma with this new product number. The old product number was M 5779.
- Caution: Very toxic.
- Preparation of 1 mM storage and stock solution (dissolved in 99.9% ethanol) for 2-mL O2k-chamber:
- Weigh 1 mg of myxothiazol into a small glass vial.
- Add 2.05 mL ethanol.
- Divide into 0.2 mL (use glass vials).
- Store at -20 ยฐC.
- Preparation of 1 mM storage and stock solution (dissolved in 99.9% ethanol) for 2-mL O2k-chamber:
- ยปO2k manual titrations MiPNet09.12
- Titration volume (2-mL O2k-chamber): 1 ยตL using a 10 ยตL Hamilton syringe.
- Final concentration: 0.5 ยตM.
- ยปO2k manual titrations MiPNet09.12
- Preparation of 0.25mM stock solution (dissolved in 99.9% ethanol) for 0.5-mL O2k-chamber:
- Take 100 ยตL of the 1 mM stock solution.
- Add 300 ยตL ethanol.
- Preparation of 0.25mM stock solution (dissolved in 99.9% ethanol) for 0.5-mL O2k-chamber:
- ยปO2k manual titrations MiPNet09.12
- Titration volume (0.5-mL O2k-chamber): 1 ยตL using a 10 ยตL Hamilton syringe.
- Final concentration: 0.5 ยตM.
- ยปO2k manual titrations MiPNet09.12
Compare
- Antimycin A is less expensive than Myx but induces higher ROS production.
MitoPedia topics:
Inhibitor