Mutuel 2010 Virology: Difference between revisions
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{{Publication | {{Publication | ||
|title=Mutuel D, Ravallec M, Chabi B, Multeau C, Salmon JM, Fournier P, Ogliastro M (2010) Pathogenesis of Junonia coenia densovirus in Spodoptera frugiperda: a route of infection that leads to hypoxia. Virology 403 | |title=Mutuel D, Ravallec M, Chabi B, Multeau C, Salmon JM, Fournier P, Ogliastro M (2010) Pathogenesis of ''Junonia coenia'' densovirus in ''Spodoptera frugiperda'': a route of infection that leads to hypoxia. Virology 403:137-44. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/20457461 PMID:20457461] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/20457461 PMID:20457461 Open Access] | ||
|authors=Mutuel D, Ravallec M, Chabi B, Multeau C, Salmon JM, Fournier P, Ogliastro M | |authors=Mutuel D, Ravallec M, Chabi B, Multeau C, Salmon JM, Fournier P, Ogliastro M | ||
|year=2010 | |year=2010 | ||
|journal=Virology | |journal=Virology | ||
|abstract=To evaluate densovirus potential against lepidopteran pests and their capacity to invade new hosts, we have characterised in vivo the infection and pathogenesis of the Junonia coenia densovirus (JcDNV) in the noctuid | |abstract=To evaluate densovirus potential against lepidopteran pests and their capacity to invade new hosts, we have characterised ''in vivo'' the infection and pathogenesis of the ''Junonia coenia'' densovirus (JcDNV) in the noctuid | ||
pest Spodoptera frugiperda. Here we show that infection starts with the ingestion of viral particles that cross the midgut epithelium without replicating. By quantitative PCR we established the kinetic and the route of | pest ''Spodoptera frugiperda''. Here we show that infection starts with the ingestion of viral particles that cross the midgut epithelium without replicating. By quantitative PCR we established the kinetic and the route of | ||
infection, from virus ingestion to replication in visceral tracheae and hemocytes. JcDNV has a high particle-to-infection ratio mostly due to the barrier function of the midgut. Pathology and cytopathology suggested | infection, from virus ingestion to replication in visceral tracheae and hemocytes. JcDNV has a high particle-to-infection ratio mostly due to the barrier function of the midgut. Pathology and cytopathology suggested | ||
that infection of tracheal cells impairs oxygen delivery to demanding tissues leading to cytopathic effects in all the tissues. Finally, larval death results from several physiological shocks, including molting arrest and anoxia. | that infection of tracheal cells impairs oxygen delivery to demanding tissues leading to cytopathic effects in all the tissues. Finally, larval death results from several physiological shocks, including molting arrest and anoxia. | ||
|keywords=Spodoptera frugiperda larvae, | |keywords=''Spodoptera frugiperda larvae'', Insect parvovirus, Densovirus, Host range, Biological control, Pathogenesis, Tissue tropism, Anoxia, ROS, Insect trachea | ||
|mipnetlab= | |mipnetlab=FR Montpellier Wrutniak-Cabello C, FR Montpellier Salmon JM | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=mt-Medicine | |||
|injuries=Ischemia-reperfusion, Oxidative stress;RONS | |||
|organism=Hexapods | |||
|preparations=Intact organism | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
| | |additional=Virus, FR | ||
}} | }} |
Latest revision as of 04:27, 29 February 2020
Mutuel D, Ravallec M, Chabi B, Multeau C, Salmon JM, Fournier P, Ogliastro M (2010) Pathogenesis of Junonia coenia densovirus in Spodoptera frugiperda: a route of infection that leads to hypoxia. Virology 403:137-44. |
Mutuel D, Ravallec M, Chabi B, Multeau C, Salmon JM, Fournier P, Ogliastro M (2010) Virology
Abstract: To evaluate densovirus potential against lepidopteran pests and their capacity to invade new hosts, we have characterised in vivo the infection and pathogenesis of the Junonia coenia densovirus (JcDNV) in the noctuid pest Spodoptera frugiperda. Here we show that infection starts with the ingestion of viral particles that cross the midgut epithelium without replicating. By quantitative PCR we established the kinetic and the route of infection, from virus ingestion to replication in visceral tracheae and hemocytes. JcDNV has a high particle-to-infection ratio mostly due to the barrier function of the midgut. Pathology and cytopathology suggested that infection of tracheal cells impairs oxygen delivery to demanding tissues leading to cytopathic effects in all the tissues. Finally, larval death results from several physiological shocks, including molting arrest and anoxia. โข Keywords: Spodoptera frugiperda larvae, Insect parvovirus, Densovirus, Host range, Biological control, Pathogenesis, Tissue tropism, Anoxia, ROS, Insect trachea
โข O2k-Network Lab: FR Montpellier Wrutniak-Cabello C, FR Montpellier Salmon JM
Labels: MiParea: mt-Medicine
Stress:Ischemia-reperfusion, Oxidative stress;RONS Organism: Hexapods
Preparation: Intact organism
HRR: Oxygraph-2k
Virus, FR